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Strain Name:
C57BL/6J-MtgxR1541Btlr/Mmmh
Stock Number:
039580-MU
Citation ID:
RRID:MMRRC_039580-MU
Other Names:
R1541 (G1), C57BL/6J-MtgxR1541Btlr
Major Collection:

Strain Information

Npr1
Name: natriuretic peptide receptor 1
Synonyms: NPRA, GC-A, NPR-A, guanylyl cyclase-A
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18160
HGNC: HGNC:7943
Homologene: 37367
Tfap2b
Name: transcription factor AP-2 beta
Synonyms: AP-2(beta), E130018K07Rik, Tcfap2b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21419
Homologene: 20688
Ece1
Name: endothelin converting enzyme 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230857
HGNC: HGNC:3146
Homologene: 1068
Cwf19l2
Name: CWF19 like cell cycle control factor 2
Synonyms: 3230401L03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244672
Homologene: 12366
Nsmce2
Name: NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase
Synonyms: 1110014D18Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68501
VEGA: 15
Homologene: 12271
Llgl2
Name: LLGL2 scribble cell polarity complex component
Synonyms: 9130006H11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217325
HGNC: HGNC:6629
Homologene: 3323
Ythdf1
Name: YTH N6-methyladenosine RNA binding protein 1
Synonyms: 2210410K23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228994
Homologene: 9844
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 19,234,070 bp
  • G to A, chromosome 1 at 38,813,276 bp
  • T to C, chromosome 1 at 93,818,275 bp
  • A to G, chromosome 2 at 31,438,240 bp
  • G to A, chromosome 2 at 33,246,165 bp
  • A to T, chromosome 2 at 67,512,290 bp
  • T to C, chromosome 2 at 143,938,488 bp
  • A to T, chromosome 2 at 155,415,304 bp
  • C to T, chromosome 2 at 164,237,009 bp
  • A to T, chromosome 2 at 167,056,190 bp
  • A to C, chromosome 2 at 180,919,143 bp
  • A to G, chromosome 2 at 181,378,865 bp
  • T to A, chromosome 3 at 15,320,684 bp
  • T to C, chromosome 3 at 87,741,841 bp
  • T to C, chromosome 3 at 90,458,744 bp
  • A to G, chromosome 4 at 3,904,085 bp
  • A to G, chromosome 4 at 9,281,814 bp
  • A to C, chromosome 4 at 19,910,645 bp
  • A to G, chromosome 4 at 55,008,928 bp
  • A to C, chromosome 4 at 82,950,518 bp
  • C to T, chromosome 4 at 107,371,288 bp
  • A to T, chromosome 4 at 118,483,873 bp
  • A to G, chromosome 4 at 136,543,325 bp
  • T to A, chromosome 4 at 137,948,660 bp
  • A to G, chromosome 4 at 148,593,424 bp
  • T to A, chromosome 5 at 105,118,409 bp
  • T to A, chromosome 5 at 120,710,346 bp
  • C to T, chromosome 6 at 51,457,605 bp
  • A to C, chromosome 6 at 124,327,961 bp
  • T to C, chromosome 7 at 3,816,989 bp
  • C to A, chromosome 7 at 10,725,052 bp
  • T to A, chromosome 7 at 16,078,512 bp
  • A to T, chromosome 7 at 56,135,657 bp
  • G to A, chromosome 7 at 61,202,603 bp
  • T to G, chromosome 7 at 81,300,631 bp
  • T to C, chromosome 7 at 107,990,841 bp
  • G to A, chromosome 7 at 118,855,786 bp
  • G to T, chromosome 7 at 120,022,886 bp
  • A to G, chromosome 8 at 14,030,688 bp
  • A to G, chromosome 8 at 46,634,334 bp
  • T to C, chromosome 8 at 88,245,599 bp
  • T to A, chromosome 9 at 3,456,760 bp
  • T to A, chromosome 9 at 58,117,466 bp
  • T to C, chromosome 9 at 64,307,489 bp
  • T to C, chromosome 9 at 107,917,363 bp
  • T to C, chromosome 10 at 34,041,663 bp
  • A to T, chromosome 10 at 58,483,094 bp
  • T to C, chromosome 10 at 120,000,543 bp
  • T to A, chromosome 11 at 51,743,796 bp
  • T to C, chromosome 11 at 74,869,190 bp
  • C to T, chromosome 11 at 107,670,048 bp
  • C to A, chromosome 11 at 115,853,121 bp
  • A to G, chromosome 12 at 55,256,059 bp
  • A to T, chromosome 12 at 91,348,781 bp
  • A to T, chromosome 12 at 112,934,928 bp
  • T to C, chromosome 12 at 113,144,966 bp
  • T to C, chromosome 13 at 8,820,538 bp
  • A to G, chromosome 13 at 42,154,376 bp
  • T to C, chromosome 13 at 46,809,213 bp
  • A to T, chromosome 14 at 32,176,888 bp
  • G to A, chromosome 14 at 32,340,667 bp
  • A to T, chromosome 14 at 47,268,192 bp
  • A to G, chromosome 14 at 79,381,485 bp
  • A to G, chromosome 14 at 103,049,333 bp
  • G to A, chromosome 14 at 118,879,478 bp
  • T to A, chromosome 15 at 59,601,385 bp
  • T to C, chromosome 16 at 18,411,815 bp
  • T to A, chromosome 16 at 36,367,501 bp
  • G to T, chromosome 17 at 24,631,976 bp
  • C to A, chromosome 17 at 30,747,247 bp
  • T to A, chromosome 17 at 35,904,331 bp
  • T to C, chromosome 18 at 68,307,782 bp
  • A to T, chromosome 19 at 47,763,852 bp
  • T to C, chromosome 19 at 47,983,530 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1541 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039580-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.