Strain Name:
C57BL/6J-MtgxR1557Btlr/Mmmh
Stock Number:
039596-MU
Citation ID:
RRID:MMRRC_039596-MU
Other Names:
R1557 (G1), C57BL/6J-MtgxR1557Btlr
Major Collection:

Gene Information

Gene Symbol: Ide [MGI:96412] (Mus musculus (mouse))
Name: insulin degrading enzyme
Synonyms: 4833415K22Rik, 1300012G03Rik
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Unc93b1 [MGI:1859307] (Mus musculus (mouse))
Name: unc-93 homolog B1, TLR signaling regulator
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Elf1 [MGI:107180] (Mus musculus (mouse))
Name: E74-like factor 1
Synonyms: Elf-1
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Armc6 [MGI:1924063] (Mus musculus (mouse))
Name: armadillo repeat containing 6
Synonyms: 2410153K17Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Fkbp5 [MGI:104670] (Mus musculus (mouse))
Name: FK506 binding protein 5
Synonyms: FKBP51, Dit1, D17Ertd592e
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Msra [MGI:106916] (Mus musculus (mouse))
Name: methionine sulfoxide reductase A
Synonyms: MSR-A, 6530413P12Rik, 2310045J23Rik
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Mib1 [MGI:2443157] (Mus musculus (mouse))
Name: mindbomb E3 ubiquitin protein ligase 1
Synonyms: mind bomb-1, Mib, skeletrophin, E430019M12Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 37,260,985 bp
  • A to C, chromosome 1 at 53,181,866 bp
  • A to G, chromosome 1 at 139,468,668 bp
  • A to G, chromosome 1 at 150,734,532 bp
  • A to T, chromosome 2 at 88,412,211 bp
  • T to A, chromosome 2 at 111,328,619 bp
  • A to T, chromosome 2 at 178,395,216 bp
  • G to T, chromosome 3 at 116,618,732 bp
  • A to C, chromosome 3 at 145,891,472 bp
  • A to G, chromosome 3 at 153,850,933 bp
  • T to C, chromosome 4 at 46,846,436 bp
  • A to T, chromosome 4 at 96,470,476 bp
  • T to C, chromosome 4 at 141,025,465 bp
  • T to C, chromosome 5 at 17,479,492 bp
  • A to T, chromosome 5 at 21,670,477 bp
  • C to A, chromosome 5 at 33,216,764 bp
  • T to A, chromosome 5 at 87,007,297 bp
  • T to A, chromosome 5 at 120,676,849 bp
  • C to T, chromosome 5 at 135,218,243 bp
  • T to A, chromosome 5 at 137,637,556 bp
  • T to C, chromosome 6 at 41,130,282 bp
  • A to T, chromosome 6 at 66,776,386 bp
  • G to A, chromosome 6 at 73,049,131 bp
  • T to A, chromosome 6 at 89,844,751 bp
  • A to T, chromosome 6 at 129,732,211 bp
  • C to T, chromosome 7 at 67,559,929 bp
  • A to G, chromosome 7 at 79,714,157 bp
  • A to G, chromosome 7 at 104,960,540 bp
  • A to C, chromosome 7 at 119,450,079 bp
  • C to T, chromosome 8 at 5,091,755 bp
  • T to C, chromosome 8 at 45,759,197 bp
  • A to T, chromosome 8 at 70,225,448 bp
  • G to A, chromosome 8 at 124,948,241 bp
  • T to A, chromosome 9 at 7,140,911 bp
  • G to A, chromosome 9 at 15,332,010 bp
  • T to C, chromosome 9 at 32,461,244 bp
  • A to G, chromosome 9 at 38,930,659 bp
  • T to A, chromosome 9 at 95,871,449 bp
  • T to G, chromosome 10 at 39,145,715 bp
  • T to C, chromosome 10 at 41,903,766 bp
  • T to C, chromosome 10 at 81,247,391 bp
  • T to C, chromosome 10 at 92,984,227 bp
  • C to A, chromosome 10 at 129,501,622 bp
  • T to A, chromosome 11 at 3,976,824 bp
  • T to C, chromosome 11 at 5,215,373 bp
  • T to C, chromosome 11 at 5,801,773 bp
  • A to T, chromosome 11 at 61,249,059 bp
  • A to G, chromosome 11 at 90,656,384 bp
  • A to G, chromosome 11 at 111,025,241 bp
  • G to T, chromosome 12 at 30,587,199 bp
  • C to T, chromosome 12 at 81,315,557 bp
  • T to A, chromosome 13 at 9,878,314 bp
  • T to C, chromosome 13 at 13,190,680 bp
  • T to C, chromosome 14 at 64,123,326 bp
  • A to G, chromosome 14 at 79,567,180 bp
  • A to G, chromosome 15 at 32,460,272 bp
  • A to C, chromosome 15 at 55,388,579 bp
  • C to A, chromosome 15 at 78,386,274 bp
  • C to A, chromosome 16 at 33,088,211 bp
  • T to C, chromosome 16 at 34,314,278 bp
  • T to A, chromosome 16 at 43,971,466 bp
  • T to C, chromosome 16 at 58,465,350 bp
  • T to C, chromosome 16 at 87,462,142 bp
  • T to C, chromosome 16 at 93,608,789 bp
  • A to G, chromosome 17 at 3,554,966 bp
  • T to C, chromosome 17 at 24,399,980 bp
  • A to G, chromosome 17 at 28,402,755 bp
  • T to C, chromosome 17 at 32,975,926 bp
  • G to A, chromosome 17 at 34,848,422 bp
  • A to G, chromosome 17 at 37,211,353 bp
  • T to A, chromosome 18 at 10,798,474 bp
  • T to C, chromosome 18 at 12,513,731 bp
  • T to A, chromosome 19 at 3,942,403 bp
  • T to C, chromosome 19 at 37,280,761 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1557 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039596-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.