Strain Name:
C57BL/6J-MtgxR1564Btlr/Mmmh
Stock Number:
039603-MU
Citation ID:
RRID:MMRRC_039603-MU
Other Names:
R1564 (G1), C57BL/6J-MtgxR1564Btlr
Major Collection:

Gene Information

Gene Symbol: Nefh [MGI:97309] (Mus musculus (mouse))
Name: neurofilament, heavy polypeptide
Synonyms: NF-H, NEFH, NF200
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Tnrc6b [MGI:2443730] (Mus musculus (mouse))
Name: trinucleotide repeat containing 6b
Synonyms: 2700090M07Rik, A730065C02Rik, D230019K20Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Cacna1h [MGI:1928842] (Mus musculus (mouse))
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Gldc [MGI:1341155] (Mus musculus (mouse))
Name: glycine decarboxylase
Synonyms: D030049L12Rik, b2b2679Clo, D19Wsu57e
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Slc17a5 [MGI:1924105] (Mus musculus (mouse))
Name: solute carrier family 17 (anion/sugar transporter), member 5
Synonyms: 4631416G20Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Scn3a [MGI:98249] (Mus musculus (mouse))
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: Nav1.3, LOC381367
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Macf1 [MGI:108559] (Mus musculus (mouse))
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 57,946,224 bp
  • A to C, chromosome 1 at 59,168,220 bp
  • G to T, chromosome 1 at 163,939,984 bp
  • A to G, chromosome 1 at 169,498,793 bp
  • A to T, chromosome 2 at 30,285,621 bp
  • C to A, chromosome 2 at 65,514,635 bp
  • A to G, chromosome 2 at 66,484,304 bp
  • C to A, chromosome 2 at 76,724,532 bp
  • A to G, chromosome 2 at 76,944,040 bp
  • G to A, chromosome 2 at 83,880,284 bp
  • A to T, chromosome 2 at 88,756,656 bp
  • G to T, chromosome 2 at 89,249,672 bp
  • A to T, chromosome 2 at 105,297,914 bp
  • C to A, chromosome 2 at 120,268,903 bp
  • G to T, chromosome 2 at 156,154,257 bp
  • G to T, chromosome 2 at 181,233,228 bp
  • T to C, chromosome 2 at 181,718,940 bp
  • T to C, chromosome 3 at 36,089,429 bp
  • A to T, chromosome 3 at 36,575,795 bp
  • G to T, chromosome 3 at 51,694,460 bp
  • T to C, chromosome 3 at 84,116,431 bp
  • T to C, chromosome 3 at 87,702,510 bp
  • C to T, chromosome 3 at 89,849,614 bp
  • T to A, chromosome 3 at 109,163,503 bp
  • C to A, chromosome 3 at 136,213,841 bp
  • T to C, chromosome 4 at 43,912,061 bp
  • C to A, chromosome 4 at 110,955,564 bp
  • T to G, chromosome 4 at 112,625,998 bp
  • T to C, chromosome 4 at 123,459,357 bp
  • T to G, chromosome 4 at 148,651,186 bp
  • A to T, chromosome 4 at 155,075,593 bp
  • T to C, chromosome 5 at 34,827,342 bp
  • T to C, chromosome 5 at 86,154,407 bp
  • C to T, chromosome 5 at 110,281,196 bp
  • C to T, chromosome 5 at 117,338,194 bp
  • C to T, chromosome 6 at 34,306,535 bp
  • T to C, chromosome 6 at 119,241,195 bp
  • A to C, chromosome 6 at 131,659,822 bp
  • A to G, chromosome 7 at 12,684,751 bp
  • A to T, chromosome 7 at 31,291,775 bp
  • G to T, chromosome 7 at 44,586,649 bp
  • A to T, chromosome 7 at 107,571,139 bp
  • A to G, chromosome 7 at 128,766,281 bp
  • G to A, chromosome 7 at 140,638,054 bp
  • T to A, chromosome 8 at 20,897,581 bp
  • A to G, chromosome 8 at 22,630,341 bp
  • G to T, chromosome 8 at 71,690,291 bp
  • A to T, chromosome 8 at 84,064,482 bp
  • T to A, chromosome 8 at 86,517,486 bp
  • A to G, chromosome 8 at 91,131,081 bp
  • A to T, chromosome 8 at 110,978,458 bp
  • C to A, chromosome 9 at 22,055,859 bp
  • G to A, chromosome 9 at 28,903,316 bp
  • A to T, chromosome 9 at 78,578,699 bp
  • C to A, chromosome 9 at 79,613,840 bp
  • T to C, chromosome 9 at 86,792,196 bp
  • T to C, chromosome 9 at 98,354,166 bp
  • T to A, chromosome 10 at 5,842,567 bp
  • T to C, chromosome 10 at 18,591,704 bp
  • A to G, chromosome 10 at 45,369,281 bp
  • T to G, chromosome 10 at 77,942,999 bp
  • T to C, chromosome 10 at 129,660,015 bp
  • T to C, chromosome 11 at 4,939,878 bp
  • C to T, chromosome 11 at 9,434,316 bp
  • T to A, chromosome 11 at 74,084,722 bp
  • A to T, chromosome 11 at 98,098,486 bp
  • A to T, chromosome 11 at 98,327,424 bp
  • C to A, chromosome 11 at 106,345,541 bp
  • A to T, chromosome 12 at 69,581,104 bp
  • A to G, chromosome 12 at 72,487,053 bp
  • T to C, chromosome 13 at 19,729,300 bp
  • T to C, chromosome 13 at 21,983,353 bp
  • C to T, chromosome 13 at 24,675,785 bp
  • C to T, chromosome 13 at 73,686,124 bp
  • A to T, chromosome 14 at 8,230,154 bp
  • T to C, chromosome 14 at 55,622,275 bp
  • T to C, chromosome 14 at 56,552,066 bp
  • T to C, chromosome 14 at 70,134,769 bp
  • A to G, chromosome 14 at 103,169,851 bp
  • A to G, chromosome 15 at 80,880,168 bp
  • A to G, chromosome 15 at 81,956,200 bp
  • C to T, chromosome 16 at 45,662,153 bp
  • G to C, chromosome 17 at 24,686,668 bp
  • A to T, chromosome 17 at 25,377,861 bp
  • C to G, chromosome 17 at 32,675,791 bp
  • A to G, chromosome 17 at 34,533,310 bp
  • G to A, chromosome 17 at 71,225,060 bp
  • G to T, chromosome 18 at 34,315,149 bp
  • A to G, chromosome 18 at 38,338,089 bp
  • G to A, chromosome 18 at 60,580,842 bp
  • A to G, chromosome 19 at 17,654,756 bp
  • A to T, chromosome 19 at 30,158,653 bp
  • A to T, chromosome 19 at 39,735,580 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1564 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039603-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.