Strain Name:
C57BL/6J-MtgxR1572Btlr/Mmmh
Stock Number:
039611-MU
Citation ID:
RRID:MMRRC_039611-MU
Other Names:
R1572 (G1), C57BL/6J-MtgxR1572Btlr
Major Collection:

Gene Information

Gene Symbol: Col3a1 [MGI:88453] (Mus musculus (mouse))
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Chrna4 [MGI:87888] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: a4 nicotinic receptor, alpha4 nAChR, alpha4-nAChR, Acra-4, Acra4
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Nrcam [MGI:104750] (Mus musculus (mouse))
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Nsd1 [MGI:1276545] (Mus musculus (mouse))
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Mthfd1 [MGI:1342005] (Mus musculus (mouse))
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: DCS, Mthfd, E430024A07Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Dop1b [MGI:1917278] (Mus musculus (mouse))
Name: DOP1 leucine zipper like protein B
Synonyms: Dopey2, 0610038M01Rik, 2610510B01Rik
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Pum1 [MGI:1931749] (Mus musculus (mouse))
Name: pumilio RNA-binding family member 1
Synonyms: Pumm
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,347,440 bp
  • T to C, chromosome 1 at 45,345,968 bp
  • T to C, chromosome 1 at 80,282,789 bp
  • G to A, chromosome 1 at 106,392,789 bp
  • A to G, chromosome 1 at 128,294,195 bp
  • T to A, chromosome 1 at 135,363,933 bp
  • C to T, chromosome 1 at 166,099,455 bp
  • T to C, chromosome 1 at 170,302,766 bp
  • T to C, chromosome 1 at 173,486,853 bp
  • T to A, chromosome 1 at 195,163,314 bp
  • T to A, chromosome 2 at 25,015,251 bp
  • A to G, chromosome 2 at 31,167,025 bp
  • T to C, chromosome 2 at 120,935,319 bp
  • T to C, chromosome 2 at 155,068,931 bp
  • T to A, chromosome 2 at 156,287,834 bp
  • A to G, chromosome 2 at 158,446,199 bp
  • T to C, chromosome 2 at 164,744,194 bp
  • T to C, chromosome 2 at 181,029,307 bp
  • A to T, chromosome 3 at 29,648,271 bp
  • A to G, chromosome 3 at 51,692,185 bp
  • T to C, chromosome 4 at 94,638,429 bp
  • T to C, chromosome 4 at 115,780,306 bp
  • T to C, chromosome 4 at 118,138,949 bp
  • T to A, chromosome 4 at 130,718,204 bp
  • A to G, chromosome 4 at 141,254,754 bp
  • T to G, chromosome 4 at 141,407,095 bp
  • G to T, chromosome 5 at 108,680,589 bp
  • A to G, chromosome 5 at 121,301,878 bp
  • A to G, chromosome 5 at 123,772,113 bp
  • T to C, chromosome 6 at 18,375,975 bp
  • T to A, chromosome 6 at 47,892,867 bp
  • T to C, chromosome 6 at 48,905,786 bp
  • C to A, chromosome 6 at 49,105,555 bp
  • T to A, chromosome 6 at 64,429,694 bp
  • A to G, chromosome 6 at 97,185,337 bp
  • A to T, chromosome 6 at 116,626,784 bp
  • T to A, chromosome 6 at 122,833,261 bp
  • T to A, chromosome 6 at 129,906,622 bp
  • A to G, chromosome 7 at 29,062,191 bp
  • A to T, chromosome 7 at 29,828,139 bp
  • G to A, chromosome 7 at 45,028,800 bp
  • T to C, chromosome 7 at 79,681,824 bp
  • T to C, chromosome 7 at 126,949,099 bp
  • T to A, chromosome 7 at 128,203,234 bp
  • T to A, chromosome 7 at 131,244,831 bp
  • C to A, chromosome 8 at 14,991,211 bp
  • A to C, chromosome 8 at 36,233,972 bp
  • T to C, chromosome 8 at 48,228,993 bp
  • A to G, chromosome 8 at 105,308,301 bp
  • A to T, chromosome 9 at 15,863,142 bp
  • G to T, chromosome 9 at 19,920,912 bp
  • A to G, chromosome 9 at 40,001,194 bp
  • G to A, chromosome 9 at 45,721,333 bp
  • G to A, chromosome 9 at 50,740,673 bp
  • A to C, chromosome 9 at 60,394,553 bp
  • A to T, chromosome 9 at 64,235,731 bp
  • T to A, chromosome 9 at 92,603,067 bp
  • A to T, chromosome 9 at 110,838,429 bp
  • A to T, chromosome 9 at 121,914,958 bp
  • T to C, chromosome 10 at 23,798,741 bp
  • A to G, chromosome 10 at 33,781,977 bp
  • C to T, chromosome 10 at 60,597,773 bp
  • A to T, chromosome 10 at 61,866,426 bp
  • T to C, chromosome 10 at 67,539,975 bp
  • A to G, chromosome 10 at 78,447,980 bp
  • C to A, chromosome 10 at 89,650,956 bp
  • A to G, chromosome 10 at 98,994,675 bp
  • G to A, chromosome 11 at 102,060,548 bp
  • G to A, chromosome 11 at 115,256,074 bp
  • T to A, chromosome 11 at 119,436,611 bp
  • G to A, chromosome 12 at 4,650,044 bp
  • A to G, chromosome 12 at 11,457,112 bp
  • T to C, chromosome 12 at 36,186,942 bp
  • T to A, chromosome 12 at 37,109,350 bp
  • T to A, chromosome 12 at 44,537,364 bp
  • A to T, chromosome 12 at 70,038,750 bp
  • T to A, chromosome 12 at 73,649,357 bp
  • G to T, chromosome 12 at 76,206,891 bp
  • C to T, chromosome 12 at 76,270,419 bp
  • A to G, chromosome 12 at 80,172,957 bp
  • T to A, chromosome 12 at 108,351,939 bp
  • T to A, chromosome 13 at 21,574,310 bp
  • T to G, chromosome 13 at 27,602,154 bp
  • T to C, chromosome 13 at 38,195,738 bp
  • T to A, chromosome 13 at 55,246,969 bp
  • T to C, chromosome 13 at 59,728,191 bp
  • T to C, chromosome 13 at 60,785,027 bp
  • T to C, chromosome 13 at 73,607,747 bp
  • T to C, chromosome 13 at 93,094,269 bp
  • T to C, chromosome 13 at 102,736,923 bp
  • T to C, chromosome 13 at 119,451,784 bp
  • A to G, chromosome 14 at 7,883,908 bp
  • A to G, chromosome 14 at 19,805,412 bp
  • A to T, chromosome 14 at 24,474,328 bp
  • G to A, chromosome 14 at 27,401,735 bp
  • A to T, chromosome 14 at 31,150,823 bp
  • A to G, chromosome 15 at 44,543,473 bp
  • T to A, chromosome 15 at 54,749,252 bp
  • A to T, chromosome 15 at 58,348,807 bp
  • T to A, chromosome 15 at 65,854,792 bp
  • C to T, chromosome 15 at 76,693,669 bp
  • T to C, chromosome 15 at 79,538,565 bp
  • A to G, chromosome 16 at 12,760,156 bp
  • A to G, chromosome 16 at 79,090,829 bp
  • A to G, chromosome 16 at 93,770,153 bp
  • G to A, chromosome 16 at 93,901,230 bp
  • A to T, chromosome 17 at 22,603,144 bp
  • A to G, chromosome 17 at 36,325,733 bp
  • A to T, chromosome 17 at 37,313,480 bp
  • A to T, chromosome 17 at 43,680,129 bp
  • A to G, chromosome 17 at 75,500,734 bp
  • T to C, chromosome 17 at 87,718,652 bp
  • A to G, chromosome 18 at 51,302,970 bp
  • A to T, chromosome 18 at 61,737,499 bp
  • T to A, chromosome 19 at 4,840,313 bp
  • G to A, chromosome 19 at 5,685,347 bp
  • C to A, chromosome 19 at 6,910,353 bp
  • A to T, chromosome 19 at 37,139,082 bp
  • T to G, chromosome 19 at 40,183,982 bp
  • T to C, chromosome 19 at 46,582,476 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1572 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039611-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.