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Strain Name:
C57BL/6J-MtgxR1594Btlr/Mmmh
Stock Number:
039631-MU
Citation ID:
RRID:MMRRC_039631-MU
Other Names:
R1594 (G1), C57BL/6J-MtgxR1594Btlr
Major Collection:

Strain Information

Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Sulf2
Name: sulfatase 2
Synonyms: 2010004N24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72043
Homologene: 10313
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 60,305,291 bp
  • T to A, chromosome 1 at 97,062,438 bp
  • A to G, chromosome 1 at 165,525,033 bp
  • T to A, chromosome 1 at 167,373,810 bp
  • A to G, chromosome 2 at 50,289,047 bp
  • A to G, chromosome 2 at 135,970,390 bp
  • T to A, chromosome 2 at 158,211,630 bp
  • A to G, chromosome 2 at 166,084,447 bp
  • C to A, chromosome 3 at 101,451,077 bp
  • G to T, chromosome 3 at 104,795,411 bp
  • A to G, chromosome 3 at 129,991,351 bp
  • T to C, chromosome 4 at 71,757,335 bp
  • C to A, chromosome 4 at 117,178,188 bp
  • A to G, chromosome 4 at 136,186,830 bp
  • T to C, chromosome 4 at 147,583,080 bp
  • T to C, chromosome 5 at 25,314,878 bp
  • C to T, chromosome 5 at 148,878,847 bp
  • T to C, chromosome 6 at 40,544,347 bp
  • T to C, chromosome 6 at 48,702,191 bp
  • C to A, chromosome 6 at 58,887,584 bp
  • A to T, chromosome 7 at 9,976,623 bp
  • A to G, chromosome 7 at 17,093,823 bp
  • G to A, chromosome 7 at 19,792,546 bp
  • G to A, chromosome 7 at 26,570,507 bp
  • C to A, chromosome 7 at 27,989,391 bp
  • A to T, chromosome 7 at 44,327,223 bp
  • A to C, chromosome 7 at 131,326,296 bp
  • A to G, chromosome 7 at 131,555,502 bp
  • T to A, chromosome 7 at 143,669,972 bp
  • T to C, chromosome 8 at 13,292,052 bp
  • A to T, chromosome 8 at 68,358,632 bp
  • A to T, chromosome 8 at 129,178,357 bp
  • A to G, chromosome 9 at 3,430,973 bp
  • A to C, chromosome 9 at 39,484,746 bp
  • A to G, chromosome 9 at 44,316,773 bp
  • A to G, chromosome 9 at 65,324,270 bp
  • A to G, chromosome 9 at 65,391,619 bp
  • T to C, chromosome 9 at 108,498,522 bp
  • A to G, chromosome 9 at 110,183,053 bp
  • A to G, chromosome 10 at 21,352,023 bp
  • A to G, chromosome 10 at 77,113,036 bp
  • A to C, chromosome 11 at 57,513,240 bp
  • T to G, chromosome 11 at 116,068,712 bp
  • A to G, chromosome 12 at 21,340,470 bp
  • A to G, chromosome 12 at 29,920,332 bp
  • G to A, chromosome 13 at 61,198,367 bp
  • T to A, chromosome 14 at 19,781,261 bp
  • T to C, chromosome 14 at 55,709,519 bp
  • T to C, chromosome 15 at 75,896,346 bp
  • A to G, chromosome 16 at 17,082,121 bp
  • G to T, chromosome 16 at 17,373,419 bp
  • G to A, chromosome 16 at 31,127,387 bp
  • T to C, chromosome 17 at 46,438,031 bp
  • A to G, chromosome 17 at 73,351,479 bp
  • A to G, chromosome 18 at 5,213,515 bp
  • A to G, chromosome 18 at 84,209,331 bp
  • C to T, chromosome 19 at 14,453,606 bp
  • T to C, chromosome 19 at 42,931,548 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1594 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039631-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.