Strain Name:
C57BL/6J-MtgxR1616Btlr/Mmmh
Stock Number:
039653-MU
Citation ID:
RRID:MMRRC_039653-MU
Other Names:
R1616 (G1), C57BL/6J-MtgxR1616Btlr
Major Collection:

Gene Information

Gene Symbol: Vcan [MGI:102889] (Mus musculus (mouse))
Name: versican
Synonyms: 5430420N07Rik, hdf, PG-M, DPEAAE, Cspg2, heart defect
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Col3a1 [MGI:88453] (Mus musculus (mouse))
Name: collagen, type III, alpha 1
Synonyms: Tsk2, Tsk-2, Col3a-1
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Ptch1 [MGI:105373] (Mus musculus (mouse))
Name: patched 1
Synonyms: wig, A230106A15Rik, Ptc, Ptc1, Patched 1
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Ltbp3 [MGI:1101355] (Mus musculus (mouse))
Name: latent transforming growth factor beta binding protein 3
Synonyms: Ltbp2
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Pcdh9 [MGI:1306801] (Mus musculus (mouse))
Name: protocadherin 9
Synonyms: C630029H24Rik, A730003J17Rik, C530050I23Rik, LOC382930, 1500001L12Rik
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Cd86 [MGI:101773] (Mus musculus (mouse))
Name: CD86 antigen
Synonyms: Cd28l2, B7-2, B70, Ly58, Ly-58, B7.2, MB7-2
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Tanc1 [MGI:1914110] (Mus musculus (mouse))
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 45,328,488 bp
  • T to C, chromosome 1 at 51,776,315 bp
  • T to C, chromosome 1 at 119,478,014 bp
  • T to C, chromosome 1 at 136,171,685 bp
  • T to C, chromosome 1 at 138,083,669 bp
  • T to A, chromosome 1 at 158,969,173 bp
  • T to C, chromosome 1 at 191,763,629 bp
  • A to G, chromosome 2 at 59,785,387 bp
  • A to G, chromosome 2 at 88,592,008 bp
  • A to G, chromosome 2 at 101,895,275 bp
  • G to A, chromosome 2 at 104,433,745 bp
  • T to C, chromosome 2 at 120,428,792 bp
  • T to C, chromosome 2 at 122,744,224 bp
  • C to T, chromosome 2 at 125,680,164 bp
  • T to C, chromosome 2 at 152,932,639 bp
  • G to A, chromosome 3 at 94,346,260 bp
  • G to T, chromosome 3 at 96,631,730 bp
  • T to C, chromosome 3 at 107,330,881 bp
  • A to G, chromosome 3 at 144,596,881 bp
  • T to C, chromosome 4 at 11,544,954 bp
  • T to A, chromosome 4 at 66,839,480 bp
  • T to C, chromosome 4 at 119,136,306 bp
  • C to G, chromosome 4 at 130,378,043 bp
  • T to C, chromosome 4 at 147,331,257 bp
  • C to A, chromosome 5 at 20,609,326 bp
  • A to T, chromosome 5 at 30,166,715 bp
  • T to G, chromosome 5 at 30,871,563 bp
  • G to T, chromosome 5 at 34,650,413 bp
  • T to C, chromosome 5 at 41,808,715 bp
  • A to C, chromosome 5 at 52,546,638 bp
  • T to A, chromosome 5 at 72,529,037 bp
  • T to A, chromosome 5 at 100,386,195 bp
  • A to T, chromosome 5 at 103,565,237 bp
  • T to A, chromosome 5 at 125,484,526 bp
  • T to G, chromosome 5 at 139,718,208 bp
  • A to G, chromosome 5 at 142,472,236 bp
  • A to T, chromosome 6 at 39,643,133 bp
  • G to A, chromosome 6 at 58,472,058 bp
  • A to T, chromosome 6 at 73,100,112 bp
  • A to G, chromosome 7 at 4,148,903 bp
  • T to G, chromosome 7 at 6,460,745 bp
  • A to T, chromosome 7 at 26,384,437 bp
  • A to G, chromosome 7 at 28,611,078 bp
  • A to G, chromosome 7 at 44,846,896 bp
  • T to A, chromosome 7 at 102,948,514 bp
  • A to T, chromosome 7 at 125,318,543 bp
  • T to A, chromosome 7 at 141,216,732 bp
  • T to C, chromosome 8 at 24,978,638 bp
  • C to G, chromosome 8 at 66,681,400 bp
  • A to T, chromosome 9 at 44,152,900 bp
  • T to C, chromosome 9 at 57,135,509 bp
  • T to A, chromosome 9 at 75,296,017 bp
  • C to A, chromosome 9 at 107,931,936 bp
  • T to C, chromosome 9 at 123,632,101 bp
  • T to C, chromosome 10 at 20,311,341 bp
  • T to G, chromosome 10 at 39,075,450 bp
  • C to A, chromosome 10 at 71,256,693 bp
  • A to T, chromosome 10 at 86,885,718 bp
  • T to G, chromosome 10 at 100,568,836 bp
  • A to G, chromosome 10 at 108,260,867 bp
  • A to G, chromosome 10 at 128,482,793 bp
  • T to C, chromosome 11 at 32,381,441 bp
  • T to C, chromosome 11 at 34,363,848 bp
  • T to A, chromosome 11 at 50,832,646 bp
  • T to G, chromosome 11 at 63,087,198 bp
  • A to G, chromosome 11 at 78,358,866 bp
  • A to G, chromosome 11 at 82,879,361 bp
  • A to G, chromosome 11 at 90,656,351 bp
  • C to T, chromosome 11 at 94,668,209 bp
  • T to C, chromosome 11 at 115,796,145 bp
  • T to C, chromosome 12 at 16,972,985 bp
  • T to G, chromosome 12 at 40,669,045 bp
  • G to T, chromosome 12 at 87,273,111 bp
  • A to T, chromosome 12 at 112,157,246 bp
  • G to T, chromosome 13 at 63,539,842 bp
  • A to G, chromosome 13 at 67,296,311 bp
  • G to A, chromosome 13 at 89,705,663 bp
  • G to A, chromosome 13 at 91,772,767 bp
  • G to A, chromosome 13 at 91,859,209 bp
  • T to C, chromosome 13 at 106,953,450 bp
  • A to G, chromosome 14 at 29,982,644 bp
  • A to T, chromosome 14 at 30,987,137 bp
  • G to T, chromosome 14 at 65,235,507 bp
  • A to T, chromosome 14 at 93,886,969 bp
  • C to T, chromosome 15 at 9,306,244 bp
  • T to A, chromosome 15 at 11,022,128 bp
  • T to A, chromosome 15 at 11,036,303 bp
  • C to T, chromosome 15 at 77,389,606 bp
  • T to C, chromosome 15 at 80,452,385 bp
  • T to C, chromosome 15 at 101,054,116 bp
  • T to C, chromosome 15 at 101,560,591 bp
  • C to T, chromosome 16 at 15,808,028 bp
  • T to C, chromosome 16 at 36,628,976 bp
  • T to C, chromosome 16 at 36,754,481 bp
  • T to A, chromosome 17 at 5,339,294 bp
  • T to A, chromosome 17 at 32,542,968 bp
  • A to G, chromosome 17 at 56,179,415 bp
  • T to A, chromosome 18 at 42,688,310 bp
  • T to C, chromosome 18 at 73,640,262 bp
  • A to G, chromosome 19 at 5,746,967 bp
  • A to G, chromosome 19 at 9,008,987 bp
  • A to G, chromosome 19 at 22,982,712 bp
  • A to G, chromosome 19 at 31,934,775 bp
  • G to A, chromosome 19 at 42,767,185 bp
  • T to A, chromosome 19 at 56,389,823 bp
  • A to G, chromosome X at 20,645,991 bp
  • C to T, chromosome X at 48,519,644 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1616 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039653-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.