Strain Name:
C57BL/6J-MtgxR1634Btlr/Mmmh
Stock Number:
039671-MU
Citation ID:
RRID:MMRRC_039671-MU
Other Names:
R1634 (G1), C57BL/6J-MtgxR1634Btlr
Major Collection:

Gene Information

Sphk2
Name: sphingosine kinase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 56632
Homologene: 32456
Spns1
Name: spinster homolog 1
Synonyms: 2210013K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 73658
Homologene: 41530
Dscaml1
Name: DS cell adhesion molecule like 1
Synonyms: 4930435C18Rik, 4921507G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 114873
VEGA: 9
Homologene: 79549
Axin1
Name: axin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 12005
HGNC: HGNC:903
Homologene: 2614
Zfp638
Name: zinc finger protein 638
Synonyms: Zfml, Np220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 18139
Homologene: 7447
Rapgef6
Name: Rap guanine nucleotide exchange factor (GEF) 6
Synonyms: Pdzgef2, PDZ-GEF2, C030018K18Rik, RA-GEF-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192786
Homologene: 22968
Cdc5l
Name: cell division cycle 5-like (S. pombe)
Synonyms: PCDC5RP, 1200002I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 71702
VEGA: 17
HGNC: HGNC:1743
Homologene: 13291
Celf1
Name: CUGBP, Elav-like family member 1
Synonyms: CUG-BP, CUG-BP1, 1600010O03Rik, Brunol2, D2Wsu101e, Cugbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 13046
HGNC: HGNC:2549
Homologene: 134102
Sbno2
Name: strawberry notch 2
Synonyms: Stno
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216161
VEGA: 10
Homologene: 8981
Leo1
Name: Leo1, Paf1/RNA polymerase II complex component
Synonyms: LOC235497
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235497
VEGA: 9
Homologene: 133895
Zfp106
Name: zinc finger protein 106
Synonyms: Cd-1, sirm, Sh3bp3, H3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20402
Homologene: 40787
Ric8b
Name: RIC8 guanine nucleotide exchange factor B
Synonyms: Ric-8b, Ric-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237422
VEGA: 10
Homologene: 23080
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Rab27a
Name: RAB27A, member RAS oncogene family
Synonyms: 4933437C11Rik, 2410003M20Rik, 2210402C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 11891
HGNC: HGNC:9766
Homologene: 3069
Map3k20
Name: mitogen-activated protein kinase kinase kinase 20
Synonyms: MLTKbeta, MLTKalpha, Zak, B230120H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 65964
Homologene: 32331
Ahi1
Name: Abelson helper integration site 1
Synonyms: 1700015F03Rik, Ahi-1, Jouberin, D10Bwg0629e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 52906
VEGA: 10
Homologene: 9762
Tjp1
Name: tight junction protein 1
Synonyms: ZO1, ZO-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 21872
Homologene: 2445
Tra2a
Name: transformer 2 alpha
Synonyms: 1500010G04Rik, G430041M01Rik, mAWMS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 101214
Homologene: 40866
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Tcf25
Name: transcription factor 25 (basic helix-loop-helix)
Synonyms: 1100001J13Rik, Nulp1, D8Ertd325e, 1810041K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 66855
Homologene: 5701
Kif24
Name: kinesin family member 24
Synonyms: 4933425J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 109242
Homologene: 52346
Mpp7
Name: membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7)
Synonyms: 5430426E14Rik, 2810038M04Rik, 1110068J02Rik, LOC381166
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 75739
VEGA: 18
Homologene: 1455
Cep152
Name: centrosomal protein 152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 99100
Homologene: 37159
Sec22a
Name: SEC22 homolog A, vesicle trafficking protein
Synonyms: Sec22l2, 1810005C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 317717
Homologene: 8246
Susd2
Name: sushi domain containing 2
Synonyms: 1200011D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 71733
VEGA: 10
Homologene: 10481
Galnt12
Name: polypeptide N-acetylgalactosaminyltransferase 12
Synonyms: A630062B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230145
Homologene: 11637
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Nell1
Name: NEL-like 1
Synonyms: B230343H07Rik, l7R6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 338352
HGNC: HGNC:7750
Homologene: 4486
Map3k5
Name: mitogen-activated protein kinase kinase kinase 5
Synonyms: ASK, ASK1, 7420452D20Rik, Mekk5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 26408
HGNC: HGNC:6857
Homologene: 38114
Fat2
Name: FAT atypical cadherin 2
Synonyms: Fath2, mKIAA0811, LOC245827, EMI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Flt1
Name: FMS-like tyrosine kinase 1
Synonyms: vascular endothelial growth factor receptor-1, VEGFR-1, Flt-1, VEGFR1, sFlt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14254
HGNC: HGNC:3763
Homologene: 134179
Mink1
Name: misshapen-like kinase 1 (zebrafish)
Synonyms: Ysk2, Map4k6, MINK, Misshapen/NIKs-related kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 50932
Homologene: 56762
D430041D05Rik
Name: RIKEN cDNA D430041D05 gene
Synonyms: G2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241589
Homologene: 115928
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 22635
Homologene: 124417
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 380698
Homologene: 70869
Scn9a
Name: sodium channel, voltage-gated, type IX, alpha
Synonyms: PN1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20274
Homologene: 2237
AI182371
Name: expressed sequence AI182371
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 98870
Homologene: 87268
Prkcg
Name: protein kinase C, gamma
Synonyms: Pkcc, PKCgamma, Prkcc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18752
HGNC: HGNC:9402
Homologene: 20602
Adam34
Name: a disintegrin and metallopeptidase domain 34
Synonyms: testase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 252866
Homologene: 78104
Armc4
Name: armadillo repeat containing 4
Synonyms: b2b643Clo, 4930463I21Rik, b2b227.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 74934
VEGA: 18
Homologene: 9992
Topaz1
Name: testis and ovary specific PAZ domain containing 1
Synonyms: Gm9524
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 671232
VEGA: 9
Homologene: 19835
Idh3b
Name: isocitrate dehydrogenase 3 (NAD+) beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 170718
HGNC: HGNC:5385
Homologene: 5035
Adam2
Name: a disintegrin and metallopeptidase domain 2
Synonyms: fertilin beta, Ftnb, Ph30-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 11495
VEGA: 14
HGNC: HGNC:198
Homologene: 1127
Sytl2
Name: synaptotagmin-like 2
Synonyms: Slp2-a, Slp2-c, Slp2, Slp2-d, Slp2-b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 83671
Homologene: 131343
Gzmc
Name: granzyme C
Synonyms: Ctla-5, Ctla5, CCP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 14940
VEGA: 14
Homologene: 105844
Adam22
Name: a disintegrin and metallopeptidase domain 22
Synonyms: 2900022I03Rik, MDC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11496
HGNC: HGNC:201
Homologene: 37898
Cttnbp2
Name: cortactin binding protein 2
Synonyms: ORF4, 3010022N24Rik, Cortbp2, 9130022E09Rik, 4732477G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 30785
Homologene: 14125
Snx14
Name: sorting nexin 14
Synonyms: C330035N22Rik, YR-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 244962
Homologene: 17823
Rgl1
Name: ral guanine nucleotide dissociation stimulator,-like 1
Synonyms: Rgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 19731
Homologene: 9039
Dzank1
Name: double zinc ribbon and ankyrin repeat domains 1
Synonyms: 2810039F03Rik, 6330439K17Rik, Ankrd64
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241688
Homologene: 10037
Dgki
Name: diacylglycerol kinase, iota
Synonyms: C130010K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 320127
HGNC: HGNC:2855
Homologene: 37956
Masp2
Name: mannan-binding lectin serine peptidase 2
Synonyms: MASP-2, MAp19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 17175
HGNC: HGNC:6902
Homologene: 4819
Olfr1295
Name: olfactory receptor 1295
Synonyms: GA_x6K02T2Q125-72616944-72616006, MOR248-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258398
Homologene: 74058
Cpne4
Name: copine IV
Synonyms: 3632411M23Rik, 4933406O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 74020
HGNC: HGNC:2317
Homologene: 26078
Olfml2a
Name: olfactomedin-like 2A
Synonyms: photomedin-1, 4932431K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241327
Homologene: 35248
Hoxb4
Name: homeobox B4
Synonyms: Hox-2.6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 15412
HGNC: HGNC:5115
Homologene: 32095
Fam189b
Name: family with sequence similarity 189, member B
Synonyms: 1110013L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 68521
HGNC: HGNC:1233
Homologene: 4805
Ripor1
Name: RHO family interacting cell polarization regulator 1
Synonyms: 2310066E14Rik, Fam65a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 75687
Homologene: 11564
Uox
Name: urate oxidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 22262
Homologene: 7584
Rinl
Name: Ras and Rab interactor-like
Synonyms: 5830482F20Rik, 9930116N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 320435
Homologene: 18556
AB512673.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
Tmem176b
Name: transmembrane protein 176B
Synonyms: 1810009M01Rik, Clast1, Lr8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 65963
Homologene: 8521
Tex261
Name: testis expressed gene 261
Synonyms: TEG-261, 3110001O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 21766
Homologene: 6131
Olfr161
Name: olfactory receptor 161
Synonyms: MOR131-1, GA_x54KRFPKG5P-112942-113883
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 258859
Homologene: 51784
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 152,524,772 bp
  • A to T, chromosome 2 at 35,086,485 bp
  • A to T, chromosome 2 at 38,960,219 bp
  • A to G, chromosome 2 at 66,488,017 bp
  • G to A, chromosome 2 at 72,410,177 bp
  • A to G, chromosome 2 at 76,722,782 bp
  • G to T, chromosome 2 at 91,013,411 bp
  • A to G, chromosome 2 at 104,221,211 bp
  • T to C, chromosome 2 at 111,565,346 bp
  • G to A, chromosome 2 at 120,533,677 bp
  • C to T, chromosome 2 at 125,583,889 bp
  • C to T, chromosome 2 at 130,281,745 bp
  • G to A, chromosome 2 at 144,481,669 bp
  • A to C, chromosome 3 at 89,188,094 bp
  • G to A, chromosome 3 at 146,612,383 bp
  • A to G, chromosome 4 at 41,393,529 bp
  • G to T, chromosome 4 at 47,108,585 bp
  • A to G, chromosome 4 at 148,614,355 bp
  • G to A, chromosome 5 at 8,121,039 bp
  • A to T, chromosome 5 at 137,412,790 bp
  • A to T, chromosome 5 at 147,676,430 bp
  • A to T, chromosome 6 at 18,408,657 bp
  • A to G, chromosome 6 at 36,915,490 bp
  • A to G, chromosome 6 at 48,834,566 bp
  • C to T, chromosome 6 at 49,250,957 bp
  • A to T, chromosome 6 at 83,775,022 bp
  • A to T, chromosome 6 at 83,979,912 bp
  • A to G, chromosome 7 at 3,323,470 bp
  • G to A, chromosome 7 at 28,796,960 bp
  • T to A, chromosome 7 at 45,711,540 bp
  • A to G, chromosome 7 at 50,848,558 bp
  • A to G, chromosome 7 at 65,302,952 bp
  • A to T, chromosome 7 at 90,395,182 bp
  • C to T, chromosome 7 at 126,371,171 bp
  • A to T, chromosome 8 at 43,652,090 bp
  • A to C, chromosome 8 at 105,615,414 bp
  • T to A, chromosome 8 at 123,397,091 bp
  • A to G, chromosome 9 at 45,672,749 bp
  • T to C, chromosome 9 at 66,473,538 bp
  • T to C, chromosome 9 at 73,075,569 bp
  • T to C, chromosome 9 at 75,466,260 bp
  • T to C, chromosome 9 at 88,385,739 bp
  • T to C, chromosome 9 at 88,407,490 bp
  • T to C, chromosome 9 at 104,989,579 bp
  • C to T, chromosome 9 at 122,780,675 bp
  • T to C, chromosome 10 at 20,136,911 bp
  • T to C, chromosome 10 at 20,965,693 bp
  • A to G, chromosome 10 at 75,637,555 bp
  • C to A, chromosome 10 at 80,060,634 bp
  • G to A, chromosome 10 at 84,970,748 bp
  • TG to TGG, chromosome 11 at 54,546,397 bp
  • T to A, chromosome 11 at 55,267,684 bp
  • C to T, chromosome 11 at 55,284,719 bp
  • T to C, chromosome 11 at 59,076,896 bp
  • T to C, chromosome 11 at 70,608,880 bp
  • C to A, chromosome 11 at 96,320,273 bp
  • T to C, chromosome 14 at 56,232,280 bp
  • A to T, chromosome 14 at 66,057,731 bp
  • A to G, chromosome 16 at 3,593,209 bp
  • T to C, chromosome 16 at 35,318,873 bp
  • T to A, chromosome 17 at 26,187,991 bp
  • T to C, chromosome 17 at 30,713,098 bp
  • A to T, chromosome 17 at 45,404,706 bp
  • A to G, chromosome 18 at 7,286,688 bp
  • A to T, chromosome 18 at 7,350,984 bp
  • C to A, chromosome X at 170,675,829 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1634 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039671-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.