Strain Name:
C57BL/6J-MtgxR1655Btlr/Mmmh
Stock Number:
039691-MU
Citation ID:
RRID:MMRRC_039691-MU
Other Names:
R1655 (G1), C57BL/6J-MtgxR1655Btlr
Major Collection:

Gene Information

Gene Symbol: Rgs14 [MGI:1859709] (Mus musculus (mouse))
Name: regulator of G-protein signaling 14
Synonyms: Rap1/rap2 interacting protein
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Dst [MGI:104627] (Mus musculus (mouse))
Name: dystonin
Synonyms: A830042E19Rik, ah, 2310001O04Rik, Bpag, bullous pemphigoid antigen 1, athetoid, nmf203, nmf339, bullous pemphigoid antigen 1, Macf2, BPAG1-n, Bpag1, BPAG1
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Rictor [MGI:1926007] (Mus musculus (mouse))
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: D530039E11Rik, 6030405M08Rik, 4921505C17Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Top1 [MGI:98788] (Mus musculus (mouse))
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Ncoa7 [MGI:2444847] (Mus musculus (mouse))
Name: nuclear receptor coactivator 7
Synonyms: 9030406N13Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Zfp609 [MGI:2674092] (Mus musculus (mouse))
Name: zinc finger protein 609
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Virma [MGI:1913435] (Mus musculus (mouse))
Name: vir like m6A methyltransferase associated
Synonyms: 1110037F02Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,584,129 bp
  • G to T, chromosome 1 at 34,282,576 bp
  • A to T, chromosome 1 at 38,811,824 bp
  • A to G, chromosome 1 at 63,661,198 bp
  • G to T, chromosome 1 at 66,672,756 bp
  • G to A, chromosome 1 at 83,277,515 bp
  • C to A, chromosome 1 at 91,448,768 bp
  • A to G, chromosome 1 at 107,046,212 bp
  • A to G, chromosome 1 at 150,630,333 bp
  • G to A, chromosome 1 at 155,132,455 bp
  • A to T, chromosome 1 at 183,087,004 bp
  • T to C, chromosome 2 at 25,460,316 bp
  • A to G, chromosome 2 at 37,331,939 bp
  • A to T, chromosome 2 at 39,080,117 bp
  • C to T, chromosome 2 at 58,052,584 bp
  • A to G, chromosome 2 at 86,228,080 bp
  • T to A, chromosome 2 at 160,703,696 bp
  • T to A, chromosome 2 at 160,910,866 bp
  • A to T, chromosome 2 at 164,803,108 bp
  • T to C, chromosome 2 at 181,234,147 bp
  • A to T, chromosome 3 at 14,107,236 bp
  • T to C, chromosome 3 at 38,957,318 bp
  • T to C, chromosome 4 at 11,494,786 bp
  • C to T, chromosome 4 at 53,050,964 bp
  • A to G, chromosome 4 at 88,816,660 bp
  • A to G, chromosome 4 at 118,734,999 bp
  • T to A, chromosome 5 at 23,747,800 bp
  • T to C, chromosome 5 at 53,069,419 bp
  • T to A, chromosome 5 at 105,081,692 bp
  • T to A, chromosome 5 at 110,335,922 bp
  • G to T, chromosome 6 at 48,753,176 bp
  • C to T, chromosome 6 at 73,205,732 bp
  • T to C, chromosome 6 at 88,100,944 bp
  • A to G, chromosome 6 at 124,437,427 bp
  • T to A, chromosome 6 at 131,220,211 bp
  • T to G, chromosome 6 at 146,376,148 bp
  • T to G, chromosome 6 at 147,060,228 bp
  • G to T, chromosome 7 at 16,141,135 bp
  • T to A, chromosome 7 at 19,645,867 bp
  • A to T, chromosome 7 at 26,449,651 bp
  • A to T, chromosome 7 at 28,147,110 bp
  • T to C, chromosome 7 at 28,330,024 bp
  • A to T, chromosome 7 at 80,598,776 bp
  • A to T, chromosome 7 at 108,103,464 bp
  • T to A, chromosome 7 at 128,115,163 bp
  • A to T, chromosome 7 at 139,642,520 bp
  • T to C, chromosome 7 at 140,860,895 bp
  • T to A, chromosome 8 at 68,373,689 bp
  • A to G, chromosome 8 at 122,496,822 bp
  • C to T, chromosome 9 at 15,340,883 bp
  • A to T, chromosome 9 at 51,083,621 bp
  • A to G, chromosome 9 at 65,703,554 bp
  • A to C, chromosome 9 at 110,632,872 bp
  • T to C, chromosome 10 at 30,698,245 bp
  • C to T, chromosome 10 at 81,213,397 bp
  • C to T, chromosome 10 at 127,177,929 bp
  • T to A, chromosome 11 at 23,375,051 bp
  • T to A, chromosome 11 at 23,429,380 bp
  • A to G, chromosome 11 at 69,473,854 bp
  • C to A, chromosome 11 at 72,257,378 bp
  • T to A, chromosome 11 at 94,048,569 bp
  • T to A, chromosome 13 at 55,383,534 bp
  • T to C, chromosome 14 at 7,934,710 bp
  • A to T, chromosome 14 at 55,560,333 bp
  • A to G, chromosome 14 at 55,651,695 bp
  • A to G, chromosome 14 at 61,191,782 bp
  • A to T, chromosome 14 at 121,465,899 bp
  • T to A, chromosome 15 at 6,772,212 bp
  • T to C, chromosome 15 at 58,873,227 bp
  • C to T, chromosome 15 at 63,780,043 bp
  • T to G, chromosome 15 at 66,828,568 bp
  • T to C, chromosome 15 at 75,108,563 bp
  • G to T, chromosome 15 at 89,196,340 bp
  • T to G, chromosome 15 at 89,419,887 bp
  • T to C, chromosome 16 at 57,571,851 bp
  • A to T, chromosome 17 at 23,177,266 bp
  • G to T, chromosome 17 at 35,382,905 bp
  • T to C, chromosome 17 at 40,831,596 bp
  • T to C, chromosome 19 at 6,343,099 bp
  • T to A, chromosome 19 at 56,843,216 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1655 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039691-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.