Strain Name:
C57BL/6J-MtgxR1664Btlr/Mmmh
Stock Number:
039700-MU
Citation ID:
RRID:MMRRC_039700-MU
Other Names:
R1664 (G1), C57BL/6J-MtgxR1664Btlr
Major Collection:

Gene Information

Gene Symbol: Wrn [MGI:109635] (Mus musculus (mouse))
Name: Werner syndrome RecQ like helicase
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Chrnb3 [MGI:106212] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, beta polypeptide 3
Synonyms: Acrb3, 5730417K16Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Plekha7 [MGI:2445094] (Mus musculus (mouse))
Name: pleckstrin homology domain containing, family A member 7
Synonyms: A430081P20Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Tmem63b [MGI:2387609] (Mus musculus (mouse))
Name: transmembrane protein 63b
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Fryl [MGI:1919563] (Mus musculus (mouse))
Name: FRY like transcription coactivator
Synonyms: 2510002A14Rik, 2310004H21Rik, 9030227G01Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Fgfr1 [MGI:95522] (Mus musculus (mouse))
Name: fibroblast growth factor receptor 1
Synonyms: Fgfr-1, Flt-2, FGFR-I, Hspy, Eask, Fr1
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Gfpt2 [MGI:1338883] (Mus musculus (mouse))
Name: glutamine fructose-6-phosphate transaminase 2
Synonyms: GFAT2
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 44,059,227 bp
  • T to C, chromosome 1 at 46,826,109 bp
  • A to T, chromosome 1 at 86,356,040 bp
  • A to G, chromosome 1 at 90,098,075 bp
  • A to C, chromosome 1 at 92,885,191 bp
  • A to G, chromosome 1 at 173,332,866 bp
  • A to T, chromosome 1 at 177,775,673 bp
  • G to A, chromosome 1 at 178,932,139 bp
  • A to G, chromosome 2 at 62,387,050 bp
  • G to A, chromosome 2 at 76,718,025 bp
  • T to C, chromosome 2 at 76,828,509 bp
  • T to A, chromosome 2 at 120,720,506 bp
  • A to G, chromosome 2 at 121,337,182 bp
  • C to A, chromosome 2 at 125,566,254 bp
  • A to T, chromosome 2 at 132,869,966 bp
  • C to T, chromosome 2 at 153,015,328 bp
  • A to G, chromosome 3 at 84,969,171 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • C to T, chromosome 3 at 100,095,531 bp
  • T to C, chromosome 3 at 104,870,898 bp
  • G to A, chromosome 3 at 145,389,600 bp
  • T to A, chromosome 3 at 146,512,148 bp
  • G to A, chromosome 4 at 14,775,079 bp
  • T to C, chromosome 4 at 43,578,080 bp
  • G to A, chromosome 4 at 63,380,375 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • T to C, chromosome 4 at 135,552,550 bp
  • A to T, chromosome 4 at 154,159,405 bp
  • A to T, chromosome 5 at 21,929,086 bp
  • A to G, chromosome 5 at 35,506,743 bp
  • A to T, chromosome 5 at 72,255,161 bp
  • A to G, chromosome 5 at 73,059,435 bp
  • A to T, chromosome 5 at 77,207,008 bp
  • C to T, chromosome 5 at 108,104,476 bp
  • C to A, chromosome 5 at 149,595,287 bp
  • G to T, chromosome 6 at 30,554,315 bp
  • T to A, chromosome 6 at 46,530,130 bp
  • T to A, chromosome 6 at 91,765,252 bp
  • C to A, chromosome 6 at 120,762,026 bp
  • T to C, chromosome 6 at 142,764,547 bp
  • T to A, chromosome 7 at 29,140,263 bp
  • T to A, chromosome 7 at 31,359,039 bp
  • T to A, chromosome 7 at 35,607,126 bp
  • T to A, chromosome 7 at 38,764,396 bp
  • C to A, chromosome 7 at 116,135,034 bp
  • A to G, chromosome 7 at 122,124,392 bp
  • A to G, chromosome 7 at 141,087,647 bp
  • T to A, chromosome 8 at 3,619,068 bp
  • T to G, chromosome 8 at 25,555,780 bp
  • A to G, chromosome 8 at 27,385,204 bp
  • A to T, chromosome 8 at 33,280,766 bp
  • T to G, chromosome 8 at 91,022,790 bp
  • T to C, chromosome 8 at 106,573,232 bp
  • A to C, chromosome 8 at 110,989,498 bp
  • C to A, chromosome 9 at 21,120,353 bp
  • A to T, chromosome 9 at 38,378,252 bp
  • A to G, chromosome 9 at 65,890,448 bp
  • A to T, chromosome 9 at 70,823,361 bp
  • A to T, chromosome 9 at 86,755,647 bp
  • A to G, chromosome 9 at 96,996,213 bp
  • A to C, chromosome 9 at 119,521,177 bp
  • G to A, chromosome 10 at 80,717,737 bp
  • A to T, chromosome 10 at 81,487,721 bp
  • C to T, chromosome 10 at 93,707,353 bp
  • A to G, chromosome 10 at 107,806,576 bp
  • A to G, chromosome 11 at 36,078,584 bp
  • G to T, chromosome 11 at 49,823,930 bp
  • T to C, chromosome 11 at 58,317,312 bp
  • T to A, chromosome 11 at 68,536,106 bp
  • C to T, chromosome 11 at 98,042,594 bp
  • A to T, chromosome 11 at 99,145,691 bp
  • C to T, chromosome 11 at 106,567,782 bp
  • T to A, chromosome 12 at 76,436,254 bp
  • T to C, chromosome 12 at 117,161,709 bp
  • T to C, chromosome 13 at 3,941,634 bp
  • A to T, chromosome 13 at 49,711,775 bp
  • A to T, chromosome 13 at 67,258,080 bp
  • T to A, chromosome 14 at 35,811,815 bp
  • T to C, chromosome 14 at 88,468,322 bp
  • A to G, chromosome 15 at 84,086,617 bp
  • C to A, chromosome 15 at 95,429,863 bp
  • T to C, chromosome 15 at 101,796,335 bp
  • A to T, chromosome 16 at 17,142,628 bp
  • T to A, chromosome 16 at 36,509,965 bp
  • A to G, chromosome 16 at 46,118,001 bp
  • A to T, chromosome 16 at 90,788,082 bp
  • G to C, chromosome 17 at 18,244,144 bp
  • C to T, chromosome 17 at 21,928,439 bp
  • T to C, chromosome 17 at 29,369,299 bp
  • T to A, chromosome 17 at 33,066,518 bp
  • T to C, chromosome 17 at 34,732,978 bp
  • C to T, chromosome 17 at 37,499,425 bp
  • T to C, chromosome 17 at 37,952,893 bp
  • T to A, chromosome 17 at 42,714,414 bp
  • A to T, chromosome 17 at 45,666,718 bp
  • G to T, chromosome 17 at 58,293,990 bp
  • A to G, chromosome 17 at 75,524,177 bp
  • A to G, chromosome 18 at 65,349,873 bp
  • T to C, chromosome 19 at 4,287,240 bp
  • A to T, chromosome 19 at 47,268,382 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1664 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039700-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.