Strain Name:
C57BL/6J-MtgxR1670Btlr/Mmmh
Stock Number:
039706-MU
Citation ID:
RRID:MMRRC_039706-MU
Other Names:
R1670 (G1), C57BL/6J-MtgxR1670Btlr
Major Collection:

Gene Information

Gene Symbol: Sema3e [MGI:1340034] (Mus musculus (mouse))
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Synonyms: Semah
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Rprd2 [MGI:1922387] (Mus musculus (mouse))
Name: regulation of nuclear pre-mRNA domain containing 2
Synonyms: 6720469I21Rik, 4930535B03Rik, 2810036A19Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Nup188 [MGI:2446190] (Mus musculus (mouse))
Name: nucleoporin 188
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Rnf38 [MGI:1920719] (Mus musculus (mouse))
Name: ring finger protein 38
Synonyms: 2610202O07Rik, 1700065B19Rik, Oip1
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Lactb2 [MGI:2442551] (Mus musculus (mouse))
Name: lactamase, beta 2
Synonyms: E430032H21Rik, Cgi-83
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Slc37a1 [MGI:2446181] (Mus musculus (mouse))
Name: solute carrier family 37 (glycerol-3-phosphate transporter), member 1
Synonyms: G3PP
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Gdnf [MGI:107430] (Mus musculus (mouse))
Name: glial cell line derived neurotrophic factor
Synonyms: glial cell line-derived neurotrophic factor
Chromosome: 15
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 13,660,417 bp
  • G to A, chromosome 1 at 13,700,943 bp
  • A to G, chromosome 1 at 56,636,389 bp
  • A to G, chromosome 1 at 85,941,415 bp
  • T to A, chromosome 1 at 173,270,900 bp
  • T to A, chromosome 1 at 190,942,163 bp
  • T to C, chromosome 2 at 30,340,655 bp
  • T to A, chromosome 2 at 86,075,250 bp
  • C to T, chromosome 2 at 111,944,919 bp
  • G to T, chromosome 2 at 130,041,768 bp
  • G to A, chromosome 2 at 130,712,379 bp
  • T to C, chromosome 2 at 163,562,576 bp
  • T to C, chromosome 2 at 163,615,366 bp
  • A to T, chromosome 3 at 28,049,240 bp
  • AACAGCA to AACAGCAACAGCA, chromosome 3 at 59,275,958 bp
  • A to G, chromosome 3 at 82,045,460 bp
  • T to C, chromosome 3 at 89,348,510 bp
  • C to T, chromosome 3 at 94,878,849 bp
  • G to T, chromosome 3 at 95,764,803 bp
  • A to T, chromosome 3 at 108,940,162 bp
  • A to C, chromosome 4 at 3,560,627 bp
  • A to G, chromosome 4 at 21,865,893 bp
  • A to G, chromosome 4 at 33,368,660 bp
  • A to G, chromosome 4 at 44,138,681 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • T to C, chromosome 4 at 151,079,771 bp
  • A to T, chromosome 5 at 5,586,409 bp
  • A to G, chromosome 5 at 5,677,393 bp
  • C to T, chromosome 5 at 14,162,185 bp
  • T to A, chromosome 5 at 23,999,991 bp
  • A to G, chromosome 5 at 137,143,995 bp
  • A to G, chromosome 6 at 65,703,070 bp
  • T to C, chromosome 6 at 115,923,883 bp
  • A to G, chromosome 6 at 142,594,722 bp
  • C to G, chromosome 6 at 147,075,191 bp
  • C to T, chromosome 7 at 45,703,525 bp
  • A to G, chromosome 7 at 102,670,402 bp
  • T to C, chromosome 7 at 113,836,813 bp
  • T to A, chromosome 7 at 126,589,475 bp
  • T to A, chromosome 7 at 130,180,457 bp
  • A to G, chromosome 7 at 139,679,316 bp
  • A to T, chromosome 8 at 84,844,119 bp
  • G to A, chromosome 8 at 121,975,997 bp
  • A to T, chromosome 9 at 6,993,942 bp
  • G to A, chromosome 9 at 54,548,369 bp
  • G to A, chromosome 9 at 56,897,403 bp
  • T to A, chromosome 9 at 95,861,456 bp
  • A to T, chromosome 9 at 110,085,273 bp
  • A to G, chromosome 10 at 80,611,141 bp
  • T to A, chromosome 10 at 86,776,252 bp
  • A to T, chromosome 11 at 58,529,411 bp
  • A to G, chromosome 11 at 59,029,377 bp
  • A to C, chromosome 11 at 73,222,931 bp
  • A to C, chromosome 12 at 116,722,172 bp
  • C to T, chromosome 13 at 55,421,533 bp
  • A to C, chromosome 13 at 100,027,433 bp
  • T to C, chromosome 14 at 30,982,427 bp
  • T to A, chromosome 14 at 33,932,043 bp
  • T to A, chromosome 14 at 69,174,677 bp
  • T to C, chromosome 15 at 7,815,649 bp
  • T to A, chromosome 15 at 32,548,799 bp
  • A to T, chromosome 15 at 76,242,070 bp
  • C to T, chromosome 15 at 78,672,368 bp
  • C to T, chromosome 15 at 81,777,067 bp
  • C to A, chromosome 15 at 89,185,337 bp
  • T to C, chromosome 15 at 91,245,815 bp
  • G to A, chromosome 16 at 4,628,233 bp
  • A to G, chromosome 16 at 37,290,927 bp
  • A to T, chromosome 16 at 59,329,244 bp
  • T to C, chromosome 16 at 59,345,427 bp
  • G to A, chromosome 16 at 97,412,135 bp
  • G to T, chromosome 17 at 19,362,252 bp
  • A to T, chromosome 17 at 30,725,124 bp
  • A to G, chromosome 17 at 31,300,521 bp
  • T to C, chromosome 17 at 49,096,577 bp
  • T to C, chromosome 17 at 55,954,479 bp
  • A to G, chromosome 17 at 78,839,100 bp
  • A to G, chromosome 19 at 5,673,315 bp
  • C to T, chromosome 19 at 5,972,848 bp
  • C to T, chromosome 19 at 11,774,595 bp
  • C to T, chromosome 19 at 29,332,068 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1670 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039706-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.