Strain Name:
C57BL/6J-MtgxR1755Btlr/Mmmh
Stock Number:
039787-MU
Citation ID:
RRID:MMRRC_039787-MU
Other Names:
R1755 (G1), C57BL/6J-MtgxR1755Btlr
Major Collection:

Gene Information

Gene Symbol: Atg7 [MGI:1921494] (Mus musculus (mouse))
Name: autophagy related 7
Synonyms: Apg7l, 1810013K23Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Epha4 [MGI:98277] (Mus musculus (mouse))
Name: Eph receptor A4
Synonyms: Hek8, Cek8, Tyro1, 2900005C20Rik, rb, Sek1, Sek
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Sparcl1 [MGI:108110] (Mus musculus (mouse))
Name: SPARC-like 1
Synonyms: Ecm2, mast9, hevin, Sc1
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Dysf [MGI:1349385] (Mus musculus (mouse))
Name: dysferlin
Synonyms: 2310004N10Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Gpr37l1 [MGI:1928503] (Mus musculus (mouse))
Name: G protein-coupled receptor 37-like 1
Synonyms: CAG-18, D0Kist8
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Utp20 [MGI:1917933] (Mus musculus (mouse))
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Zbtb8a [MGI:1920930] (Mus musculus (mouse))
Name: zinc finger and BTB domain containing 8a
Synonyms: 2410081M15Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 63,543,170 bp
  • A to T, chromosome 1 at 77,387,823 bp
  • T to C, chromosome 1 at 130,883,943 bp
  • A to G, chromosome 1 at 131,058,350 bp
  • A to G, chromosome 1 at 135,166,901 bp
  • G to A, chromosome 1 at 170,681,777 bp
  • C to A, chromosome 1 at 173,677,910 bp
  • T to A, chromosome 2 at 6,884,958 bp
  • T to C, chromosome 2 at 26,359,534 bp
  • T to A, chromosome 2 at 28,550,546 bp
  • T to A, chromosome 2 at 57,109,092 bp
  • T to C, chromosome 2 at 60,234,921 bp
  • A to G, chromosome 2 at 66,501,716 bp
  • A to T, chromosome 2 at 120,535,175 bp
  • T to C, chromosome 2 at 120,984,477 bp
  • T to A, chromosome 2 at 169,582,804 bp
  • T to A, chromosome 3 at 28,713,662 bp
  • C to A, chromosome 3 at 69,034,108 bp
  • G to A, chromosome 4 at 34,575,114 bp
  • A to G, chromosome 4 at 34,811,043 bp
  • T to C, chromosome 4 at 129,354,317 bp
  • T to C, chromosome 4 at 143,850,810 bp
  • G to T, chromosome 5 at 53,872,247 bp
  • C to T, chromosome 5 at 104,092,824 bp
  • T to G, chromosome 5 at 110,207,541 bp
  • T to A, chromosome 5 at 135,729,485 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • T to G, chromosome 6 at 40,926,162 bp
  • G to A, chromosome 6 at 84,070,214 bp
  • A to G, chromosome 6 at 114,673,677 bp
  • T to A, chromosome 6 at 136,929,614 bp
  • A to G, chromosome 7 at 4,751,938 bp
  • A to T, chromosome 7 at 5,611,303 bp
  • T to C, chromosome 7 at 15,834,520 bp
  • T to C, chromosome 7 at 90,160,549 bp
  • A to T, chromosome 7 at 118,203,064 bp
  • A to G, chromosome 7 at 143,569,457 bp
  • T to C, chromosome 8 at 45,397,973 bp
  • A to G, chromosome 8 at 69,814,124 bp
  • G to A, chromosome 8 at 87,588,957 bp
  • T to C, chromosome 8 at 95,724,577 bp
  • G to A, chromosome 9 at 31,177,104 bp
  • T to A, chromosome 9 at 71,261,741 bp
  • A to T, chromosome 10 at 29,222,294 bp
  • A to G, chromosome 10 at 86,824,251 bp
  • A to G, chromosome 10 at 88,809,769 bp
  • A to G, chromosome 10 at 123,133,044 bp
  • T to C, chromosome 11 at 74,364,993 bp
  • AC to A, chromosome 11 at 84,276,564 bp
  • A to G, chromosome 11 at 115,013,775 bp
  • T to A, chromosome 12 at 11,091,444 bp
  • A to G, chromosome 12 at 116,226,029 bp
  • A to G, chromosome 13 at 46,752,613 bp
  • A to T, chromosome 13 at 46,773,678 bp
  • G to T, chromosome 13 at 48,885,743 bp
  • A to G, chromosome 14 at 61,310,944 bp
  • A to T, chromosome 15 at 28,326,636 bp
  • T to C, chromosome 15 at 55,016,454 bp
  • A to T, chromosome 15 at 65,995,421 bp
  • T to A, chromosome 15 at 74,838,534 bp
  • G to A, chromosome 15 at 78,966,479 bp
  • A to G, chromosome 15 at 85,797,979 bp
  • A to G, chromosome 15 at 95,972,570 bp
  • T to G, chromosome 16 at 18,823,253 bp
  • A to G, chromosome 16 at 24,845,124 bp
  • T to G, chromosome 16 at 38,780,040 bp
  • G to A, chromosome 16 at 49,706,190 bp
  • G to A, chromosome 17 at 18,582,653 bp
  • T to C, chromosome 17 at 54,292,978 bp
  • A to T, chromosome 17 at 67,855,502 bp
  • A to G, chromosome 18 at 77,012,067 bp
  • T to C, chromosome 18 at 89,009,317 bp
  • A to G, chromosome 19 at 4,623,574 bp
  • A to T, chromosome 19 at 45,758,406 bp
  • A to G, chromosome 19 at 46,254,110 bp
  • A to G, chromosome Y at 1,279,543 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1755 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039787-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.