Strain Name:
C57BL/6J-MtgxR1775Btlr/Mmmh
Stock Number:
039806-MU
Citation ID:
RRID:MMRRC_039806-MU
Other Names:
R1775 (G1), C57BL/6J-MtgxR1775Btlr
Major Collection:

Gene Information

Gene Symbol: Sulf2 [MGI:1919293] (Mus musculus (mouse))
Name: sulfatase 2
Synonyms: 2010004N24Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Sp4 [MGI:107595] (Mus musculus (mouse))
Name: trans-acting transcription factor 4
Synonyms: 5730497N03Rik, HF-1b, HF1-b
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Sod2 [MGI:98352] (Mus musculus (mouse))
Name: superoxide dismutase 2, mitochondrial
Synonyms: Sod-2, MnSOD, manganese SOD, manganese superoxide dismutase
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Ntrk3 [MGI:97385] (Mus musculus (mouse))
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Neurod1 [MGI:1339708] (Mus musculus (mouse))
Name: neurogenic differentiation 1
Synonyms: Nd1, Neurod, BETA2, bHLHa3
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Ank2 [MGI:88025] (Mus musculus (mouse))
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-B, Ankyrin-2, Gm4392
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Gria2 [MGI:95809] (Mus musculus (mouse))
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: Glur-2, GluR2, GluR-B, GluA2, Glur2
Chromosome: 3
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 13,576,456 bp
  • C to A, chromosome 1 at 90,081,416 bp
  • A to G, chromosome 1 at 128,300,301 bp
  • A to G, chromosome 1 at 134,893,348 bp
  • A to G, chromosome 1 at 160,989,647 bp
  • A to G, chromosome 1 at 162,968,725 bp
  • A to G, chromosome 1 at 169,745,278 bp
  • T to C, chromosome 1 at 182,973,722 bp
  • C to A, chromosome 2 at 27,756,244 bp
  • C to T, chromosome 2 at 60,672,644 bp
  • T to A, chromosome 2 at 65,472,342 bp
  • T to C, chromosome 2 at 66,680,955 bp
  • G to T, chromosome 2 at 79,454,437 bp
  • G to A, chromosome 2 at 86,074,760 bp
  • G to A, chromosome 2 at 92,330,515 bp
  • T to A, chromosome 2 at 116,000,291 bp
  • C to A, chromosome 2 at 131,648,189 bp
  • A to T, chromosome 2 at 139,746,043 bp
  • A to G, chromosome 2 at 157,107,547 bp
  • A to G, chromosome 2 at 158,806,437 bp
  • G to A, chromosome 2 at 163,025,355 bp
  • A to T, chromosome 2 at 164,277,085 bp
  • T to C, chromosome 2 at 166,079,612 bp
  • T to C, chromosome 2 at 167,457,358 bp
  • A to G, chromosome 3 at 14,864,432 bp
  • G to T, chromosome 3 at 80,691,338 bp
  • T to C, chromosome 3 at 87,368,659 bp
  • A to G, chromosome 3 at 87,923,817 bp
  • C to T, chromosome 3 at 92,992,941 bp
  • T to A, chromosome 3 at 123,336,517 bp
  • G to A, chromosome 3 at 126,934,547 bp
  • A to T, chromosome 3 at 136,103,838 bp
  • T to A, chromosome 4 at 118,829,868 bp
  • A to G, chromosome 4 at 123,860,493 bp
  • T to C, chromosome 4 at 133,601,231 bp
  • C to T, chromosome 4 at 134,470,653 bp
  • C to T, chromosome 4 at 137,520,156 bp
  • T to A, chromosome 4 at 152,038,218 bp
  • T to C, chromosome 4 at 154,862,214 bp
  • T to C, chromosome 4 at 155,843,074 bp
  • T to C, chromosome 5 at 31,500,842 bp
  • T to C, chromosome 5 at 35,042,850 bp
  • T to C, chromosome 5 at 69,340,560 bp
  • T to A, chromosome 5 at 111,276,811 bp
  • T to A, chromosome 5 at 121,291,191 bp
  • T to C, chromosome 5 at 146,467,077 bp
  • A to T, chromosome 6 at 30,810,336 bp
  • A to G, chromosome 6 at 47,576,660 bp
  • T to C, chromosome 6 at 116,582,964 bp
  • A to T, chromosome 7 at 24,874,367 bp
  • T to A, chromosome 7 at 78,356,041 bp
  • T to C, chromosome 7 at 83,612,532 bp
  • T to C, chromosome 7 at 85,738,170 bp
  • A to C, chromosome 7 at 102,134,937 bp
  • G to A, chromosome 7 at 104,636,159 bp
  • T to C, chromosome 7 at 119,845,411 bp
  • C to T, chromosome 7 at 127,866,606 bp
  • G to T, chromosome 7 at 139,238,563 bp
  • C to A, chromosome 7 at 139,238,565 bp
  • A to T, chromosome 8 at 70,486,253 bp
  • G to A, chromosome 8 at 123,492,900 bp
  • T to C, chromosome 9 at 45,952,062 bp
  • T to A, chromosome 9 at 51,059,533 bp
  • C to T, chromosome 9 at 67,881,447 bp
  • T to C, chromosome 9 at 69,488,081 bp
  • G to A, chromosome 9 at 114,279,318 bp
  • A to G, chromosome 10 at 81,467,304 bp
  • A to G, chromosome 10 at 88,770,808 bp
  • T to A, chromosome 10 at 100,496,810 bp
  • A to G, chromosome 11 at 23,271,193 bp
  • T to C, chromosome 11 at 69,320,867 bp
  • A to G, chromosome 11 at 71,161,821 bp
  • A to G, chromosome 11 at 84,300,422 bp
  • T to C, chromosome 11 at 87,237,235 bp
  • T to A, chromosome 11 at 101,160,785 bp
  • C to G, chromosome 11 at 101,276,340 bp
  • T to C, chromosome 11 at 116,223,660 bp
  • A to G, chromosome 12 at 11,222,142 bp
  • A to G, chromosome 12 at 11,309,269 bp
  • G to T, chromosome 12 at 98,852,704 bp
  • A to T, chromosome 12 at 118,299,600 bp
  • A to G, chromosome 13 at 3,560,018 bp
  • A to G, chromosome 13 at 3,887,642 bp
  • A to G, chromosome 13 at 41,317,962 bp
  • C to A, chromosome 13 at 67,749,704 bp
  • G to T, chromosome 14 at 50,681,166 bp
  • T to C, chromosome 14 at 56,372,589 bp
  • A to T, chromosome 14 at 57,603,672 bp
  • G to A, chromosome 15 at 12,592,460 bp
  • T to C, chromosome 15 at 47,899,739 bp
  • A to G, chromosome 15 at 79,640,119 bp
  • T to C, chromosome 15 at 82,350,377 bp
  • T to C, chromosome 15 at 103,563,018 bp
  • G to A, chromosome 16 at 4,851,558 bp
  • A to G, chromosome 16 at 35,660,324 bp
  • A to T, chromosome 16 at 58,718,558 bp
  • A to G, chromosome 17 at 6,139,046 bp
  • T to A, chromosome 17 at 13,015,032 bp
  • T to A, chromosome 17 at 36,865,169 bp
  • T to C, chromosome 17 at 74,612,286 bp
  • A to G, chromosome 17 at 80,373,743 bp
  • T to A, chromosome 18 at 12,118,412 bp
  • G to T, chromosome 19 at 8,619,107 bp
  • C to A, chromosome 19 at 12,901,235 bp
  • A to T, chromosome 19 at 43,798,419 bp
  • T to A, chromosome 19 at 44,999,242 bp
  • T to A, chromosome 19 at 59,235,341 bp
  • T to C, chromosome Y at 1,368,089 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1775 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039806-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.