Strain Name:
C57BL/6J-MtgxR1776Btlr/Mmmh
Stock Number:
039807-MU
Citation ID:
RRID:MMRRC_039807-MU
Other Names:
R1776 (G1), C57BL/6J-MtgxR1776Btlr
Major Collection:

Gene Information

Gene Symbol: Epb41l2 [MGI:103009] (Mus musculus (mouse))
Name: erythrocyte membrane protein band 4.1 like 2
Synonyms: D10Ertd398e, Epb4.1l2, NBL2, 4.1G
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Kcnq2 [MGI:1309503] (Mus musculus (mouse))
Name: potassium voltage-gated channel, subfamily Q, member 2
Synonyms: Nmf134, KQT2
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Srgap2 [MGI:109605] (Mus musculus (mouse))
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: FBP2, 9930124L22Rik, Fnbp2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Nlk [MGI:1201387] (Mus musculus (mouse))
Name: nemo like kinase
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Herc4 [MGI:1914595] (Mus musculus (mouse))
Name: hect domain and RLD 4
Synonyms: 4921531D01Rik, 9530080M15Rik, 1700056O17Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Senp2 [MGI:1923076] (Mus musculus (mouse))
Name: SUMO/sentrin specific peptidase 2
Synonyms: 2310007L05Rik, 4930538C18Rik
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Setd3 [MGI:1289184] (Mus musculus (mouse))
Name: SET domain containing 3
Synonyms: D12Ertd771e, 2610102I01Rik, 2610305M23Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 13,062,340 bp
  • T to C, chromosome 1 at 40,629,287 bp
  • T to A, chromosome 1 at 131,411,850 bp
  • T to C, chromosome 1 at 131,976,956 bp
  • C to T, chromosome 1 at 156,442,421 bp
  • A to G, chromosome 1 at 171,872,750 bp
  • A to G, chromosome 1 at 188,728,203 bp
  • G to T, chromosome 1 at 191,063,188 bp
  • T to C, chromosome 2 at 32,574,755 bp
  • A to T, chromosome 2 at 112,957,253 bp
  • A to C, chromosome 2 at 125,321,734 bp
  • A to T, chromosome 2 at 130,234,869 bp
  • T to C, chromosome 2 at 153,131,212 bp
  • T to C, chromosome 2 at 158,131,459 bp
  • A to T, chromosome 2 at 172,427,695 bp
  • T to A, chromosome 2 at 181,100,557 bp
  • C to A, chromosome 3 at 87,952,994 bp
  • T to A, chromosome 3 at 89,832,038 bp
  • A to T, chromosome 3 at 105,977,798 bp
  • T to A, chromosome 3 at 144,813,920 bp
  • A to T, chromosome 4 at 9,598,773 bp
  • A to G, chromosome 4 at 15,111,267 bp
  • T to C, chromosome 4 at 21,759,475 bp
  • A to G, chromosome 4 at 58,879,100 bp
  • A to G, chromosome 4 at 59,207,775 bp
  • G to A, chromosome 4 at 87,176,289 bp
  • A to T, chromosome 4 at 106,639,040 bp
  • T to C, chromosome 4 at 141,415,189 bp
  • T to C, chromosome 5 at 6,769,806 bp
  • T to C, chromosome 5 at 8,579,050 bp
  • T to C, chromosome 5 at 29,785,093 bp
  • T to A, chromosome 5 at 53,889,311 bp
  • T to C, chromosome 5 at 115,562,465 bp
  • A to G, chromosome 6 at 42,678,455 bp
  • A to G, chromosome 6 at 56,957,038 bp
  • A to G, chromosome 6 at 85,267,945 bp
  • A to T, chromosome 6 at 89,335,464 bp
  • C to G, chromosome 6 at 121,641,424 bp
  • A to G, chromosome 7 at 102,011,050 bp
  • T to A, chromosome 7 at 121,068,299 bp
  • A to G, chromosome 7 at 123,171,297 bp
  • T to C, chromosome 7 at 126,992,761 bp
  • G to T, chromosome 8 at 71,409,274 bp
  • A to T, chromosome 9 at 13,818,874 bp
  • G to A, chromosome 9 at 20,170,809 bp
  • G to A, chromosome 9 at 38,982,894 bp
  • T to C, chromosome 9 at 50,597,200 bp
  • T to A, chromosome 9 at 58,366,728 bp
  • C to T, chromosome 9 at 65,182,752 bp
  • A to T, chromosome 9 at 116,174,967 bp
  • T to C, chromosome 10 at 5,644,992 bp
  • A to T, chromosome 10 at 7,810,437 bp
  • C to T, chromosome 10 at 24,049,648 bp
  • T to A, chromosome 10 at 25,443,733 bp
  • T to A, chromosome 10 at 63,264,171 bp
  • T to C, chromosome 10 at 70,875,228 bp
  • T to A, chromosome 10 at 86,957,816 bp
  • C to T, chromosome 10 at 107,685,089 bp
  • T to G, chromosome 10 at 114,800,603 bp
  • C to T, chromosome 10 at 129,791,131 bp
  • T to C, chromosome 11 at 4,914,517 bp
  • T to A, chromosome 11 at 78,587,027 bp
  • T to C, chromosome 11 at 89,526,474 bp
  • T to A, chromosome 11 at 116,865,877 bp
  • A to G, chromosome 12 at 59,149,575 bp
  • C to A, chromosome 12 at 108,165,161 bp
  • T to C, chromosome 12 at 110,632,928 bp
  • T to A, chromosome 12 at 115,335,390 bp
  • T to A, chromosome 12 at 118,849,567 bp
  • A to C, chromosome 13 at 11,745,176 bp
  • T to A, chromosome 13 at 38,196,617 bp
  • A to G, chromosome 13 at 59,716,567 bp
  • C to A, chromosome 13 at 62,370,074 bp
  • T to C, chromosome 13 at 112,541,239 bp
  • A to G, chromosome 14 at 24,491,762 bp
  • A to G, chromosome 14 at 30,912,743 bp
  • T to C, chromosome 14 at 55,709,397 bp
  • T to A, chromosome 14 at 65,295,386 bp
  • G to A, chromosome 14 at 79,452,605 bp
  • A to G, chromosome 15 at 73,555,101 bp
  • T to C, chromosome 15 at 78,165,981 bp
  • A to G, chromosome 15 at 83,343,139 bp
  • T to C, chromosome 16 at 22,043,060 bp
  • G to A, chromosome 16 at 31,944,579 bp
  • A to G, chromosome 16 at 34,952,782 bp
  • A to G, chromosome 16 at 87,479,316 bp
  • T to A, chromosome 18 at 36,647,308 bp
  • T to A, chromosome 18 at 58,954,620 bp
  • G to A, chromosome 18 at 61,036,014 bp
  • T to C, chromosome 18 at 67,289,517 bp
  • A to G, chromosome 19 at 55,078,575 bp
  • A to G, chromosome X at 166,406,006 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1776 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039807-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.