Strain Name:
C57BL/6J-MtgxR1777Btlr/Mmmh
Stock Number:
039808-MU
Citation ID:
RRID:MMRRC_039808-MU
Other Names:
R1777 (G1), C57BL/6J-MtgxR1777Btlr
Major Collection:

Gene Information

Gene Symbol: Cdh1 [MGI:88354] (Mus musculus (mouse))
Name: cadherin 1
Synonyms: L-CAM, E-cadherin, UM, uvomorulin, E-cad, Ecad
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Pzp [MGI:87854] (Mus musculus (mouse))
Name: PZP, alpha-2-macroglobulin like
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Srd5a3 [MGI:1930252] (Mus musculus (mouse))
Name: steroid 5 alpha-reductase 3
Synonyms: D730040M03Rik, Srd5a2l, 1110025P14Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Slc17a6 [MGI:2156052] (Mus musculus (mouse))
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms: 2900073D12Rik, VGLUT2
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Tnpo3 [MGI:1196412] (Mus musculus (mouse))
Name: transportin 3
Synonyms: 5730544L10Rik, D6Ertd313e, C430013M08Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Dnajc21 [MGI:1925371] (Mus musculus (mouse))
Name: DnaJ heat shock protein family (Hsp40) member C21
Synonyms: 9930116P15Rik, 4930461P20Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Slc29a1 [MGI:1927073] (Mus musculus (mouse))
Name: solute carrier family 29 (nucleoside transporters), member 1
Synonyms: NBMPR-sensitive equilibrative nucleoside transporter, ENT1, 1200014D21Rik
Chromosome: 17
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 16,078,589 bp
  • T to A, chromosome 1 at 100,370,078 bp
  • C to A, chromosome 1 at 174,581,922 bp
  • T to A, chromosome 2 at 32,305,470 bp
  • T to A, chromosome 2 at 111,983,697 bp
  • T to C, chromosome 2 at 118,430,839 bp
  • T to C, chromosome 2 at 125,619,894 bp
  • T to C, chromosome 2 at 158,462,195 bp
  • T to G, chromosome 2 at 180,031,795 bp
  • T to C, chromosome 3 at 68,617,684 bp
  • A to T, chromosome 4 at 11,225,600 bp
  • A to G, chromosome 4 at 85,130,679 bp
  • G to A, chromosome 4 at 129,596,949 bp
  • T to A, chromosome 4 at 154,270,690 bp
  • T to C, chromosome 5 at 3,836,799 bp
  • T to C, chromosome 5 at 38,320,161 bp
  • C to T, chromosome 5 at 64,127,782 bp
  • T to C, chromosome 5 at 65,021,591 bp
  • T to G, chromosome 5 at 76,149,783 bp
  • C to A, chromosome 5 at 107,136,930 bp
  • T to A, chromosome 5 at 108,317,391 bp
  • A to T, chromosome 5 at 109,294,270 bp
  • T to C, chromosome 5 at 125,429,931 bp
  • A to T, chromosome 5 at 136,227,422 bp
  • G to T, chromosome 5 at 142,714,062 bp
  • G to A, chromosome 6 at 17,104,755 bp
  • A to G, chromosome 6 at 29,555,139 bp
  • T to A, chromosome 6 at 82,092,156 bp
  • A to G, chromosome 6 at 87,463,307 bp
  • A to C, chromosome 6 at 88,618,060 bp
  • C to A, chromosome 6 at 91,477,330 bp
  • T to C, chromosome 6 at 128,490,572 bp
  • A to G, chromosome 6 at 132,762,291 bp
  • C to T, chromosome 7 at 4,664,872 bp
  • T to C, chromosome 7 at 19,244,912 bp
  • A to T, chromosome 7 at 22,790,430 bp
  • A to T, chromosome 7 at 41,545,719 bp
  • G to A, chromosome 7 at 43,813,329 bp
  • A to G, chromosome 7 at 44,207,451 bp
  • G to A, chromosome 7 at 44,904,152 bp
  • T to A, chromosome 7 at 49,798,346 bp
  • A to T, chromosome 7 at 51,646,209 bp
  • T to A, chromosome 7 at 86,586,064 bp
  • A to T, chromosome 7 at 90,403,052 bp
  • T to C, chromosome 7 at 103,051,376 bp
  • A to G, chromosome 8 at 18,939,132 bp
  • C to T, chromosome 8 at 25,178,002 bp
  • T to A, chromosome 8 at 27,199,826 bp
  • G to T, chromosome 8 at 48,417,179 bp
  • T to C, chromosome 8 at 77,565,108 bp
  • T to A, chromosome 8 at 84,912,068 bp
  • T to C, chromosome 8 at 85,940,943 bp
  • T to A, chromosome 8 at 94,052,062 bp
  • T to C, chromosome 8 at 94,588,811 bp
  • T to A, chromosome 8 at 106,656,835 bp
  • C to T, chromosome 8 at 110,589,571 bp
  • T to C, chromosome 8 at 119,633,487 bp
  • T to C, chromosome 9 at 35,648,116 bp
  • A to G, chromosome 9 at 37,746,311 bp
  • T to C, chromosome 9 at 45,683,756 bp
  • A to T, chromosome 9 at 55,864,546 bp
  • A to G, chromosome 9 at 104,872,688 bp
  • T to A, chromosome 9 at 108,508,201 bp
  • A to T, chromosome 9 at 110,437,401 bp
  • A to T, chromosome 9 at 111,604,082 bp
  • A to C, chromosome 9 at 116,109,880 bp
  • C to T, chromosome 9 at 119,449,818 bp
  • A to G, chromosome 9 at 120,051,593 bp
  • T to C, chromosome 10 at 7,866,217 bp
  • A to G, chromosome 10 at 22,707,843 bp
  • A to G, chromosome 10 at 40,251,272 bp
  • A to T, chromosome 10 at 79,787,385 bp
  • A to G, chromosome 11 at 60,514,936 bp
  • A to T, chromosome 11 at 72,910,272 bp
  • A to G, chromosome 11 at 86,771,978 bp
  • A to G, chromosome 11 at 87,602,830 bp
  • A to G, chromosome 11 at 94,213,754 bp
  • A to T, chromosome 12 at 13,513,562 bp
  • C to A, chromosome 12 at 85,268,754 bp
  • T to A, chromosome 12 at 103,112,455 bp
  • T to A, chromosome 13 at 23,114,325 bp
  • A to T, chromosome 13 at 59,791,821 bp
  • A to T, chromosome 13 at 67,833,865 bp
  • C to A, chromosome 14 at 34,268,173 bp
  • T to A, chromosome 14 at 50,486,300 bp
  • T to C, chromosome 14 at 56,100,601 bp
  • C to A, chromosome 15 at 10,449,607 bp
  • C to A, chromosome 15 at 73,820,214 bp
  • A to C, chromosome 15 at 99,707,810 bp
  • G to A, chromosome 16 at 21,217,235 bp
  • A to G, chromosome 16 at 37,060,224 bp
  • G to T, chromosome 16 at 44,114,705 bp
  • A to T, chromosome 16 at 44,735,853 bp
  • A to G, chromosome 16 at 59,202,016 bp
  • T to G, chromosome 16 at 73,004,667 bp
  • A to T, chromosome 16 at 73,043,194 bp
  • T to C, chromosome 16 at 77,081,554 bp
  • T to C, chromosome 16 at 79,003,318 bp
  • T to A, chromosome 16 at 92,077,756 bp
  • A to T, chromosome 17 at 12,271,730 bp
  • G to A, chromosome 17 at 33,931,744 bp
  • T to A, chromosome 17 at 35,024,948 bp
  • A to G, chromosome 17 at 45,587,308 bp
  • A to G, chromosome 18 at 34,457,760 bp
  • T to A, chromosome 18 at 37,515,718 bp
  • G to A, chromosome 19 at 10,858,506 bp
  • C to T, chromosome 19 at 20,816,109 bp
  • A to T, chromosome 19 at 33,499,321 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1777 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039808-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.