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Strain Name:
C57BL/6J-MtgxR1815Btlr/Mmmh
Stock Number:
039843-MU
Citation ID:
RRID:MMRRC_039843-MU
Other Names:
R1815 (G1), C57BL/6J-MtgxR1815Btlr
Major Collection:

Strain Information

Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: 1110006F08Rik, D12Ertd7e, Dicer1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 192119
VEGA: 12
Homologene: 13251
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Zmym1
Name: zinc finger, MYM domain containing 1
Synonyms: 5830412B09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68310
Homologene: 32904
Fastk
Name: Fas-activated serine/threonine kinase
Synonyms: 0610011K02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66587
Homologene: 4884
Srsf11
Name: serine and arginine-rich splicing factor 11
Synonyms: 0610009J05Rik, 2610019N13Rik, Sfrs11
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69207
Homologene: 36164
Prkci
Name: protein kinase C, iota
Synonyms: Pkcl, Pkci, 2310021H13Rik, Prkcl, aPKClambda, PKClambda
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18759
HGNC: HGNC:9404
Homologene: 37667
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 24,685,561 bp
  • T to C, chromosome 1 at 128,300,159 bp
  • A to T, chromosome 1 at 169,995,601 bp
  • A to G, chromosome 1 at 171,256,336 bp
  • A to G, chromosome 1 at 171,872,750 bp
  • A to T, chromosome 1 at 191,025,380 bp
  • A to C, chromosome 1 at 193,118,283 bp
  • T to C, chromosome 2 at 31,393,043 bp
  • T to A, chromosome 2 at 110,731,258 bp
  • A to G, chromosome 3 at 28,109,768 bp
  • T to C, chromosome 3 at 31,038,495 bp
  • A to T, chromosome 3 at 141,757,757 bp
  • A to T, chromosome 3 at 141,880,363 bp
  • C to T, chromosome 3 at 158,016,427 bp
  • T to G, chromosome 4 at 43,512,405 bp
  • G to T, chromosome 4 at 55,530,977 bp
  • C to T, chromosome 4 at 58,982,929 bp
  • T to C, chromosome 4 at 127,049,021 bp
  • T to C, chromosome 5 at 24,441,531 bp
  • T to A, chromosome 5 at 31,245,813 bp
  • T to A, chromosome 5 at 83,279,234 bp
  • T to C, chromosome 5 at 83,284,282 bp
  • T to A, chromosome 5 at 108,777,425 bp
  • T to C, chromosome 6 at 114,864,059 bp
  • T to A, chromosome 6 at 118,383,781 bp
  • T to C, chromosome 6 at 130,224,107 bp
  • T to A, chromosome 6 at 146,359,416 bp
  • A to G, chromosome 7 at 25,530,014 bp
  • A to T, chromosome 7 at 27,516,665 bp
  • G to A, chromosome 7 at 34,186,401 bp
  • A to G, chromosome 7 at 43,586,344 bp
  • A to G, chromosome 7 at 47,078,841 bp
  • G to T, chromosome 7 at 64,392,170 bp
  • T to A, chromosome 7 at 79,438,308 bp
  • GGCGGCGGCGGCGGCGGCGGCGGCGGCGGC to GGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGC, chromosome 7 at 97,579,906 bp
  • GCG to GCGACG, chromosome 7 at 97,579,907 bp
  • CG to CGACGGGG, chromosome 7 at 97,579,908 bp
  • A to G, chromosome 7 at 112,412,902 bp
  • T to C, chromosome 8 at 22,011,651 bp
  • T to C, chromosome 8 at 40,512,121 bp
  • T to A, chromosome 8 at 79,639,989 bp
  • C to A, chromosome 8 at 109,940,223 bp
  • G to A, chromosome 9 at 20,968,092 bp
  • T to C, chromosome 9 at 39,237,990 bp
  • T to C, chromosome 9 at 66,032,324 bp
  • A to G, chromosome 9 at 67,229,423 bp
  • A to G, chromosome 9 at 99,093,095 bp
  • C to A, chromosome 9 at 107,900,522 bp
  • T to A, chromosome 10 at 39,822,871 bp
  • A to G, chromosome 10 at 41,337,576 bp
  • T to C, chromosome 10 at 76,552,964 bp
  • T to C, chromosome 10 at 80,364,021 bp
  • A to G, chromosome 11 at 4,889,101 bp
  • T to C, chromosome 11 at 69,475,574 bp
  • T to C, chromosome 11 at 90,671,069 bp
  • T to C, chromosome 11 at 100,095,739 bp
  • G to T, chromosome 11 at 118,332,648 bp
  • T to C, chromosome 12 at 11,294,601 bp
  • T to A, chromosome 12 at 16,972,726 bp
  • T to C, chromosome 12 at 58,213,478 bp
  • T to C, chromosome 12 at 104,722,151 bp
  • T to C, chromosome 12 at 109,034,107 bp
  • A to G, chromosome 13 at 21,423,172 bp
  • A to G, chromosome 13 at 29,717,791 bp
  • A to T, chromosome 13 at 33,039,904 bp
  • C to T, chromosome 13 at 93,454,077 bp
  • T to C, chromosome 13 at 95,986,022 bp
  • A to T, chromosome 14 at 57,890,143 bp
  • A to G, chromosome 14 at 60,086,624 bp
  • A to T, chromosome 15 at 63,869,116 bp
  • A to T, chromosome 15 at 76,593,708 bp
  • T to A, chromosome 15 at 77,635,572 bp
  • A to G, chromosome 15 at 78,250,402 bp
  • A to G, chromosome 15 at 89,387,597 bp
  • A to T, chromosome 15 at 92,250,948 bp
  • A to G, chromosome 15 at 102,713,923 bp
  • A to G, chromosome 16 at 64,478,492 bp
  • T to C, chromosome 16 at 89,015,449 bp
  • T to C, chromosome 17 at 32,056,811 bp
  • T to A, chromosome 17 at 33,669,155 bp
  • T to A, chromosome 17 at 36,773,944 bp
  • G to A, chromosome 17 at 59,010,136 bp
  • A to T, chromosome 17 at 75,252,380 bp
  • G to A, chromosome 18 at 5,767,898 bp
  • A to T, chromosome 18 at 46,291,106 bp
  • A to G, chromosome 18 at 46,720,302 bp
  • A to T, chromosome 18 at 67,415,573 bp
  • G to A, chromosome 19 at 10,861,567 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1815 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039843-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.