MMRRC:039843-MU

T to A, chromosome 1 at 24,685,561 bp
T to C, chromosome 1 at 128,300,159 bp
A to T, chromosome 1 at 169,995,601 bp
A to G, chromosome 1 at 171,256,336 bp
A to G, chromosome 1 at 171,872,750 bp
A to T, chromosome 1 at 191,025,380 bp
A to C, chromosome 1 at 193,118,283 bp
T to C, chromosome 2 at 31,393,043 bp
T to A, chromosome 2 at 110,731,258 bp
A to G, chromosome 3 at 28,109,768 bp
T to C, chromosome 3 at 31,038,495 bp
A to T, chromosome 3 at 141,757,757 bp
A to T, chromosome 3 at 141,880,363 bp
C to T, chromosome 3 at 158,016,427 bp
T to G, chromosome 4 at 43,512,405 bp
G to T, chromosome 4 at 55,530,977 bp
C to T, chromosome 4 at 58,982,929 bp
T to C, chromosome 4 at 127,049,021 bp
T to C, chromosome 5 at 24,441,531 bp
T to A, chromosome 5 at 31,245,813 bp
T to A, chromosome 5 at 83,279,234 bp
T to C, chromosome 5 at 83,284,282 bp
T to A, chromosome 5 at 108,777,425 bp
T to C, chromosome 6 at 114,864,059 bp
T to A, chromosome 6 at 118,383,781 bp
T to C, chromosome 6 at 130,224,107 bp
T to A, chromosome 6 at 146,359,416 bp
A to G, chromosome 7 at 25,530,014 bp
A to T, chromosome 7 at 27,516,665 bp
G to A, chromosome 7 at 34,186,401 bp
A to G, chromosome 7 at 43,586,344 bp
A to G, chromosome 7 at 47,078,841 bp
G to T, chromosome 7 at 64,392,170 bp
T to A, chromosome 7 at 79,438,308 bp
GGCGGCGGCGGCGGCGGCGGCGGCGGCGGC to GGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGC, chromosome 7 at 97,579,906 bp
GCG to GCGACG, chromosome 7 at 97,579,907 bp
CG to CGACGGGG, chromosome 7 at 97,579,908 bp
A to G, chromosome 7 at 112,412,902 bp
T to C, chromosome 8 at 22,011,651 bp
T to C, chromosome 8 at 40,512,121 bp
T to A, chromosome 8 at 79,639,989 bp
C to A, chromosome 8 at 109,940,223 bp
G to A, chromosome 9 at 20,968,092 bp
T to C, chromosome 9 at 39,237,990 bp
T to C, chromosome 9 at 66,032,324 bp
A to G, chromosome 9 at 67,229,423 bp
A to G, chromosome 9 at 99,093,095 bp
C to A, chromosome 9 at 107,900,522 bp
T to A, chromosome 10 at 39,822,871 bp
A to G, chromosome 10 at 41,337,576 bp
T to C, chromosome 10 at 76,552,964 bp
T to C, chromosome 10 at 80,364,021 bp
A to G, chromosome 11 at 4,889,101 bp
T to C, chromosome 11 at 69,475,574 bp
T to C, chromosome 11 at 90,671,069 bp
T to C, chromosome 11 at 100,095,739 bp
G to T, chromosome 11 at 118,332,648 bp
T to C, chromosome 12 at 11,294,601 bp
T to A, chromosome 12 at 16,972,726 bp
T to C, chromosome 12 at 58,213,478 bp
T to C, chromosome 12 at 104,722,151 bp
T to C, chromosome 12 at 109,034,107 bp
A to G, chromosome 13 at 21,423,172 bp
A to G, chromosome 13 at 29,717,791 bp
A to T, chromosome 13 at 33,039,904 bp
C to T, chromosome 13 at 93,454,077 bp
T to C, chromosome 13 at 95,986,022 bp
A to T, chromosome 14 at 57,890,143 bp
A to G, chromosome 14 at 60,086,624 bp
A to T, chromosome 15 at 63,869,116 bp
A to T, chromosome 15 at 76,593,708 bp
T to A, chromosome 15 at 77,635,572 bp
A to G, chromosome 15 at 78,250,402 bp
A to G, chromosome 15 at 89,387,597 bp
A to T, chromosome 15 at 92,250,948 bp
A to G, chromosome 15 at 102,713,923 bp
A to G, chromosome 16 at 64,478,492 bp
T to C, chromosome 16 at 89,015,449 bp
T to C, chromosome 17 at 32,056,811 bp
T to A, chromosome 17 at 33,669,155 bp
T to A, chromosome 17 at 36,773,944 bp
G to A, chromosome 17 at 59,010,136 bp
A to T, chromosome 17 at 75,252,380 bp
G to A, chromosome 18 at 5,767,898 bp
A to T, chromosome 18 at 46,291,106 bp
A to G, chromosome 18 at 46,720,302 bp
A to T, chromosome 18 at 67,415,573 bp
G to A, chromosome 19 at 10,861,567 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain GQC Summary

The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.

Suggested Control Mice

Littermates of all relevant genotypes.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R1815 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
039843-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All