Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR1822Btlr/Mmmh
Stock Number:
039850-MU
Citation ID:
RRID:MMRRC_039850-MU
Other Names:
R1822 (G1), C57BL/6J-MtgxR1822Btlr
Major Collection:
Beutler Mutagenetix

Gene Information

Gene Details

Gene Symbol: Zmpste24 [MGI:1890508] (Mus musculus (mouse))
Name: zinc metallopeptidase, STE24
Synonyms: A530043O15Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Ntf3 [MGI:97380] (Mus musculus (mouse))
Name: neurotrophin 3
Synonyms: Ntf-3, NT-3, NT3
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Pdpk1 [MGI:1338068] (Mus musculus (mouse))
Name: 3-phosphoinositide dependent protein kinase 1
Synonyms: Pdk1, Pkb kinase
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Map2k5 [MGI:1346345] (Mus musculus (mouse))
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Slc6a5 [MGI:105090] (Mus musculus (mouse))
Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Synonyms: Glyt2
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Slc32a1 [MGI:1194488] (Mus musculus (mouse))
Name: solute carrier family 32 (GABA vesicular transporter), member 1
Synonyms: Viaat, R75019, VGAT
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Crhr1 [MGI:88498] (Mus musculus (mouse))
Name: corticotropin releasing hormone receptor 1
Synonyms: CRF-R1alpha, CRFR1, CRF1R, CRF 1 receptor
Chromosome: 11
Alteration at locus: Chemically Induced
See All Alterations
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 20,347,457 bp
  • T to A, chromosome 1 at 173,589,842 bp
  • C to T, chromosome 1 at 174,152,214 bp
  • C to A, chromosome 1 at 182,472,960 bp
  • T to C, chromosome 1 at 182,517,112 bp
  • A to T, chromosome 2 at 22,340,280 bp
  • C to T, chromosome 2 at 31,383,692 bp
  • C to G, chromosome 2 at 37,201,980 bp
  • T to A, chromosome 2 at 52,716,527 bp
  • A to C, chromosome 2 at 58,134,146 bp
  • A to G, chromosome 2 at 65,484,372 bp
  • T to C, chromosome 2 at 77,924,659 bp
  • A to G, chromosome 2 at 80,517,898 bp
  • T to C, chromosome 2 at 87,252,710 bp
  • A to G, chromosome 2 at 154,646,134 bp
  • T to A, chromosome 2 at 158,492,452 bp
  • A to G, chromosome 2 at 158,611,378 bp
  • G to A, chromosome 2 at 160,780,355 bp
  • T to C, chromosome 3 at 14,559,225 bp
  • T to C, chromosome 3 at 95,411,044 bp
  • T to A, chromosome 3 at 129,812,633 bp
  • A to G, chromosome 4 at 43,182,867 bp
  • A to G, chromosome 4 at 58,805,539 bp
  • T to C, chromosome 4 at 115,566,613 bp
  • A to G, chromosome 4 at 116,636,653 bp
  • T to C, chromosome 4 at 121,087,316 bp
  • T to A, chromosome 4 at 137,402,466 bp
  • G to A, chromosome 5 at 30,378,710 bp
  • G to T, chromosome 5 at 52,368,162 bp
  • T to C, chromosome 5 at 66,400,177 bp
  • A to T, chromosome 5 at 96,770,688 bp
  • T to C, chromosome 5 at 120,609,580 bp
  • A to G, chromosome 5 at 134,503,227 bp
  • T to A, chromosome 5 at 145,984,953 bp
  • T to G, chromosome 5 at 150,540,198 bp
  • A to G, chromosome 6 at 29,768,584 bp
  • A to T, chromosome 6 at 48,492,886 bp
  • G to A, chromosome 6 at 52,202,732 bp
  • T to C, chromosome 6 at 88,836,554 bp
  • C to T, chromosome 6 at 124,231,634 bp
  • A to G, chromosome 6 at 125,081,415 bp
  • G to A, chromosome 6 at 125,863,457 bp
  • A to C, chromosome 6 at 126,102,246 bp
  • A to G, chromosome 6 at 136,587,260 bp
  • T to C, chromosome 7 at 30,482,453 bp
  • T to C, chromosome 7 at 31,142,573 bp
  • A to G, chromosome 7 at 46,209,901 bp
  • T to A, chromosome 7 at 49,956,425 bp
  • T to C, chromosome 7 at 64,372,806 bp
  • T to A, chromosome 7 at 81,076,931 bp
  • T to C, chromosome 7 at 105,749,647 bp
  • T to C, chromosome 8 at 16,223,326 bp
  • C to A, chromosome 8 at 117,758,749 bp
  • T to A, chromosome 8 at 123,492,526 bp
  • G to A, chromosome 9 at 35,646,105 bp
  • T to A, chromosome 9 at 43,791,077 bp
  • C to T, chromosome 9 at 55,859,900 bp
  • A to G, chromosome 9 at 63,235,303 bp
  • A to G, chromosome 10 at 7,596,197 bp
  • G to A, chromosome 10 at 78,812,931 bp
  • A to T, chromosome 10 at 80,703,895 bp
  • C to T, chromosome 10 at 107,718,478 bp
  • T to A, chromosome 10 at 111,996,860 bp
  • G to A, chromosome 10 at 128,766,044 bp
  • T to A, chromosome 11 at 49,470,968 bp
  • A to T, chromosome 11 at 58,720,307 bp
  • A to T, chromosome 11 at 69,514,804 bp
  • A to G, chromosome 11 at 74,270,240 bp
  • A to G, chromosome 11 at 83,444,219 bp
  • A to T, chromosome 11 at 104,133,072 bp
  • A to T, chromosome 11 at 104,522,822 bp
  • A to T, chromosome 11 at 106,267,396 bp
  • G to T, chromosome 11 at 109,957,075 bp
  • A to T, chromosome 11 at 115,324,628 bp
  • C to T, chromosome 12 at 33,045,205 bp
  • T to G, chromosome 12 at 64,995,635 bp
  • T to C, chromosome 12 at 85,266,145 bp
  • C to T, chromosome 12 at 110,620,459 bp
  • T to A, chromosome 13 at 18,948,455 bp
  • A to T, chromosome 13 at 33,833,234 bp
  • A to G, chromosome 13 at 46,615,347 bp
  • C to A, chromosome 13 at 76,130,288 bp
  • A to T, chromosome 14 at 55,885,852 bp
  • T to C, chromosome 14 at 59,356,329 bp
  • A to T, chromosome 14 at 122,585,048 bp
  • G to T, chromosome 15 at 80,962,742 bp
  • T to C, chromosome 15 at 86,235,569 bp
  • C to T, chromosome 15 at 98,861,780 bp
  • G to A, chromosome 16 at 15,759,605 bp
  • G to C, chromosome 16 at 32,753,919 bp
  • C to T, chromosome 17 at 21,690,858 bp
  • A to T, chromosome 17 at 24,098,176 bp
  • C to T, chromosome 17 at 31,300,431 bp
  • A to G, chromosome 17 at 37,196,831 bp
  • A to T, chromosome 17 at 53,973,925 bp
  • T to C, chromosome 17 at 64,740,842 bp
  • A to C, chromosome 17 at 78,622,836 bp
  • A to G, chromosome 17 at 80,253,085 bp
  • G to A, chromosome 18 at 30,344,077 bp
  • A to G, chromosome 18 at 43,342,328 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • G to A, chromosome 19 at 10,263,186 bp
  • A to G, chromosome 19 at 25,161,058 bp
  • T to C, chromosome 19 at 29,716,414 bp
  • T to C, chromosome 19 at 34,039,091 bp
  • T to A, chromosome 19 at 47,257,459 bp
  • T to C, chromosome 19 at 61,089,972 bp
  • G to A, chromosome X at 73,777,955 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1822 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039850-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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