Strain Name:
C57BL/6J-MtgxR1824Btlr/Mmmh
Stock Number:
039852-MU
Citation ID:
RRID:MMRRC_039852-MU
Other Names:
R1824 (G1), C57BL/6J-MtgxR1824Btlr
Major Collection:

Gene Information

Gene Symbol: Vcan [MGI:102889] (Mus musculus (mouse))
Name: versican
Synonyms: DPEAAE, heart defect, Cspg2, PG-M, hdf, 5430420N07Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Slc17a6 [MGI:2156052] (Mus musculus (mouse))
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms: VGLUT2, 2900073D12Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Lcat [MGI:96755] (Mus musculus (mouse))
Name: lecithin cholesterol acyltransferase
Synonyms: D8Wsu61e
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Tfpi [MGI:1095418] (Mus musculus (mouse))
Name: tissue factor pathway inhibitor
Synonyms: A630013F22Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Magi1 [MGI:1203522] (Mus musculus (mouse))
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: Gukmi1, WWP3, AIP3, BAP1, Baiap1
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Spen [MGI:1891706] (Mus musculus (mouse))
Name: spen family transcription repressor
Synonyms: Mint
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Fam110b [MGI:1916593] (Mus musculus (mouse))
Name: family with sequence similarity 110, member B
Synonyms: 1700012H17Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 20,347,457 bp
  • T to A, chromosome 1 at 52,126,515 bp
  • A to C, chromosome 1 at 53,927,170 bp
  • A to C, chromosome 1 at 74,418,447 bp
  • A to G, chromosome 1 at 87,503,264 bp
  • A to G, chromosome 1 at 88,509,789 bp
  • T to C, chromosome 1 at 136,273,783 bp
  • A to G, chromosome 1 at 195,157,316 bp
  • G to A, chromosome 2 at 22,396,243 bp
  • T to A, chromosome 2 at 31,800,644 bp
  • C to T, chromosome 2 at 37,429,817 bp
  • A to G, chromosome 2 at 51,955,915 bp
  • C to T, chromosome 2 at 84,453,789 bp
  • G to T, chromosome 2 at 89,609,349 bp
  • T to C, chromosome 2 at 118,232,908 bp
  • G to A, chromosome 2 at 119,211,535 bp
  • A to C, chromosome 2 at 130,395,697 bp
  • C to A, chromosome 3 at 59,932,580 bp
  • A to G, chromosome 4 at 5,799,029 bp
  • G to T, chromosome 4 at 42,934,661 bp
  • C to A, chromosome 4 at 45,826,406 bp
  • C to A, chromosome 4 at 63,733,351 bp
  • T to C, chromosome 4 at 139,360,865 bp
  • C to A, chromosome 4 at 141,472,785 bp
  • C to T, chromosome 5 at 24,413,751 bp
  • G to T, chromosome 5 at 52,368,162 bp
  • T to C, chromosome 5 at 67,348,052 bp
  • A to T, chromosome 5 at 96,770,688 bp
  • A to G, chromosome 5 at 134,503,227 bp
  • C to T, chromosome 5 at 143,517,225 bp
  • T to A, chromosome 5 at 145,984,953 bp
  • C to T, chromosome 5 at 150,536,922 bp
  • A to T, chromosome 6 at 12,409,042 bp
  • C to T, chromosome 6 at 16,840,468 bp
  • T to A, chromosome 6 at 29,205,088 bp
  • A to G, chromosome 6 at 54,619,357 bp
  • A to T, chromosome 6 at 81,964,079 bp
  • T to C, chromosome 6 at 88,836,554 bp
  • A to G, chromosome 6 at 88,911,823 bp
  • A to G, chromosome 6 at 93,699,639 bp
  • A to G, chromosome 6 at 115,948,758 bp
  • T to A, chromosome 6 at 122,609,962 bp
  • C to T, chromosome 6 at 124,231,634 bp
  • C to T, chromosome 6 at 124,635,682 bp
  • G to A, chromosome 7 at 6,066,956 bp
  • T to C, chromosome 7 at 25,288,266 bp
  • T to G, chromosome 7 at 25,624,792 bp
  • A to G, chromosome 7 at 51,661,546 bp
  • T to A, chromosome 7 at 127,314,060 bp
  • C to A, chromosome 7 at 139,639,602 bp
  • C to G, chromosome 8 at 70,720,417 bp
  • A to T, chromosome 8 at 82,766,015 bp
  • A to G, chromosome 8 at 105,700,490 bp
  • T to C, chromosome 8 at 105,939,888 bp
  • A to T, chromosome 8 at 119,734,506 bp
  • C to T, chromosome 9 at 45,778,928 bp
  • A to G, chromosome 9 at 49,456,884 bp
  • G to C, chromosome 9 at 64,859,358 bp
  • T to A, chromosome 9 at 78,157,862 bp
  • T to C, chromosome 9 at 95,936,421 bp
  • A to G, chromosome 9 at 107,676,575 bp
  • G to T, chromosome 9 at 108,348,008 bp
  • T to C, chromosome 9 at 108,514,610 bp
  • A to G, chromosome 9 at 114,709,184 bp
  • T to C, chromosome 10 at 70,872,256 bp
  • T to C, chromosome 10 at 107,779,831 bp
  • T to C, chromosome 10 at 129,945,337 bp
  • A to G, chromosome 11 at 58,994,832 bp
  • A to G, chromosome 11 at 88,982,097 bp
  • G to A, chromosome 11 at 100,374,137 bp
  • G to T, chromosome 11 at 116,030,442 bp
  • A to G, chromosome 12 at 103,059,320 bp
  • A to G, chromosome 12 at 109,033,099 bp
  • T to A, chromosome 12 at 117,688,684 bp
  • A to T, chromosome 13 at 27,508,450 bp
  • A to T, chromosome 13 at 46,713,747 bp
  • C to T, chromosome 13 at 49,900,808 bp
  • G to T, chromosome 13 at 89,705,212 bp
  • T to A, chromosome 14 at 14,328,774 bp
  • T to A, chromosome 14 at 21,609,875 bp
  • G to T, chromosome 14 at 21,837,731 bp
  • T to C, chromosome 14 at 24,149,444 bp
  • A to T, chromosome 14 at 31,176,432 bp
  • T to C, chromosome 14 at 56,589,872 bp
  • A to G, chromosome 14 at 103,252,509 bp
  • T to C, chromosome 15 at 11,022,086 bp
  • C to A, chromosome 15 at 59,994,525 bp
  • T to C, chromosome 15 at 80,376,789 bp
  • T to C, chromosome 15 at 86,235,569 bp
  • T to C, chromosome 15 at 101,460,139 bp
  • T to G, chromosome 15 at 102,431,003 bp
  • A to G, chromosome 16 at 16,869,071 bp
  • C to T, chromosome 16 at 20,715,962 bp
  • G to T, chromosome 16 at 32,755,933 bp
  • C to T, chromosome 16 at 34,294,215 bp
  • A to T, chromosome 16 at 45,826,011 bp
  • A to G, chromosome 16 at 90,866,443 bp
  • A to C, chromosome 16 at 96,825,581 bp
  • A to G, chromosome 17 at 6,956,026 bp
  • T to A, chromosome 17 at 21,928,541 bp
  • T to A, chromosome 17 at 21,992,380 bp
  • T to C, chromosome 17 at 30,731,180 bp
  • G to A, chromosome 17 at 31,531,245 bp
  • C to T, chromosome 17 at 34,692,333 bp
  • T to C, chromosome 17 at 34,814,779 bp
  • A to G, chromosome 17 at 57,105,738 bp
  • T to C, chromosome 17 at 73,709,939 bp
  • T to C, chromosome 17 at 88,750,157 bp
  • A to G, chromosome 18 at 4,649,293 bp
  • T to A, chromosome 18 at 22,522,068 bp
  • T to A, chromosome 18 at 60,912,297 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • T to C, chromosome 19 at 29,716,414 bp
  • G to A, chromosome 19 at 34,670,878 bp
  • T to C, chromosome 19 at 56,546,718 bp
  • T to C, chromosome 19 at 56,755,325 bp
  • T to C, chromosome 19 at 61,089,972 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1824 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039852-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.