Strain Name:
C57BL/6J-MtgxR1828Btlr/Mmmh
Stock Number:
039855-MU
Citation ID:
RRID:MMRRC_039855-MU
Other Names:
R1828 (G1), C57BL/6J-MtgxR1828Btlr
Major Collection:

Gene Information

Gene Symbol: Gpr83 [MGI:95712] (Mus musculus (mouse))
Name: G protein-coupled receptor 83
Synonyms: Gir, GIR, RP105, RP82, RP39, glucocorticoid-induced receptor, Gpr72
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Pax4 [MGI:97488] (Mus musculus (mouse))
Name: paired box 4
Synonyms: Pax-4
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Olr1 [MGI:1261434] (Mus musculus (mouse))
Name: oxidized low density lipoprotein (lectin-like) receptor 1
Synonyms: LOX-1, SR-EI, Scare1
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Gaa [MGI:95609] (Mus musculus (mouse))
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Rpl3 [MGI:1351605] (Mus musculus (mouse))
Name: ribosomal protein L3
Synonyms: J1, F2
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Sfswap [MGI:101760] (Mus musculus (mouse))
Name: splicing factor SWAP
Synonyms: 1190005N23Rik, Sfrs8, 6330437E22Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Erbin [MGI:1890169] (Mus musculus (mouse))
Name: Erbb2 interacting protein
Synonyms: Erbin, 1700028E05Rik, Erbb2ip
Chromosome: 13
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 44,057,074 bp
  • T to C, chromosome 1 at 53,926,023 bp
  • CAGCTCCACGAAG to CAG, chromosome 1 at 172,071,471 bp
  • A to G, chromosome 1 at 191,098,517 bp
  • T to C, chromosome 2 at 28,579,682 bp
  • T to C, chromosome 2 at 49,680,240 bp
  • A to G, chromosome 2 at 67,515,238 bp
  • A to T, chromosome 2 at 88,818,058 bp
  • A to G, chromosome 2 at 90,138,587 bp
  • A to G, chromosome 2 at 118,638,439 bp
  • T to A, chromosome 2 at 122,347,380 bp
  • G to A, chromosome 2 at 127,256,174 bp
  • A to G, chromosome 2 at 128,628,705 bp
  • T to A, chromosome 2 at 134,530,664 bp
  • T to C, chromosome 2 at 154,148,045 bp
  • G to T, chromosome 3 at 8,947,519 bp
  • G to A, chromosome 3 at 36,638,546 bp
  • A to T, chromosome 3 at 38,983,458 bp
  • G to A, chromosome 3 at 75,902,438 bp
  • T to C, chromosome 3 at 90,733,003 bp
  • A to T, chromosome 3 at 93,526,121 bp
  • G to T, chromosome 3 at 97,746,766 bp
  • A to G, chromosome 3 at 135,246,496 bp
  • G to T, chromosome 3 at 138,107,280 bp
  • A to G, chromosome 3 at 152,242,768 bp
  • T to G, chromosome 4 at 49,645,876 bp
  • C to T, chromosome 4 at 57,910,240 bp
  • T to G, chromosome 4 at 59,610,888 bp
  • A to G, chromosome 4 at 119,255,793 bp
  • G to A, chromosome 4 at 155,965,703 bp
  • T to C, chromosome 5 at 8,969,080 bp
  • A to G, chromosome 5 at 48,304,030 bp
  • A to T, chromosome 5 at 124,761,279 bp
  • T to C, chromosome 5 at 124,836,178 bp
  • C to T, chromosome 5 at 129,513,084 bp
  • C to A, chromosome 5 at 145,291,048 bp
  • T to C, chromosome 5 at 150,445,927 bp
  • T to C, chromosome 6 at 28,443,447 bp
  • G to A, chromosome 6 at 83,107,481 bp
  • A to G, chromosome 6 at 129,353,799 bp
  • T to C, chromosome 6 at 129,488,932 bp
  • A to G, chromosome 6 at 146,328,332 bp
  • A to G, chromosome 7 at 3,159,037 bp
  • T to A, chromosome 7 at 43,639,456 bp
  • T to G, chromosome 7 at 83,791,724 bp
  • A to G, chromosome 7 at 102,477,513 bp
  • T to A, chromosome 7 at 104,861,124 bp
  • G to T, chromosome 7 at 108,630,648 bp
  • A to G, chromosome 7 at 109,766,556 bp
  • A to G, chromosome 7 at 111,043,958 bp
  • A to T, chromosome 7 at 126,875,838 bp
  • T to A, chromosome 7 at 127,391,909 bp
  • T to C, chromosome 7 at 143,576,648 bp
  • T to C, chromosome 8 at 13,585,035 bp
  • A to T, chromosome 8 at 110,510,894 bp
  • A to T, chromosome 8 at 123,823,826 bp
  • G to T, chromosome 9 at 14,868,333 bp
  • T to C, chromosome 9 at 48,326,614 bp
  • A to T, chromosome 9 at 53,376,641 bp
  • T to A, chromosome 9 at 69,482,978 bp
  • T to C, chromosome 9 at 79,628,353 bp
  • A to T, chromosome 10 at 19,660,317 bp
  • T to C, chromosome 11 at 87,612,117 bp
  • C to A, chromosome 11 at 116,977,788 bp
  • T to C, chromosome 11 at 119,283,272 bp
  • C to T, chromosome 11 at 121,117,491 bp
  • G to A, chromosome 13 at 19,594,603 bp
  • G to A, chromosome 13 at 43,668,534 bp
  • A to G, chromosome 13 at 103,860,069 bp
  • T to A, chromosome 13 at 113,368,808 bp
  • C to T, chromosome 14 at 8,151,507 bp
  • A to T, chromosome 14 at 20,462,664 bp
  • C to A, chromosome 14 at 23,330,929 bp
  • A to T, chromosome 14 at 26,911,840 bp
  • A to T, chromosome 14 at 45,287,760 bp
  • G to A, chromosome 14 at 70,572,037 bp
  • A to G, chromosome 14 at 76,007,703 bp
  • T to C, chromosome 15 at 78,386,388 bp
  • C to T, chromosome 15 at 80,080,390 bp
  • T to C, chromosome 15 at 85,819,282 bp
  • A to G, chromosome 16 at 17,280,750 bp
  • A to C, chromosome 16 at 22,956,103 bp
  • A to G, chromosome 17 at 24,366,197 bp
  • T to G, chromosome 17 at 32,911,235 bp
  • C to T, chromosome 17 at 37,851,075 bp
  • T to C, chromosome 17 at 40,880,071 bp
  • T to C, chromosome 17 at 46,653,202 bp
  • T to A, chromosome 17 at 83,503,968 bp
  • T to A, chromosome 18 at 37,295,985 bp
  • T to C, chromosome 19 at 6,397,607 bp
  • C to T, chromosome X at 143,103,015 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1828 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039855-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.