Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR1832Btlr/Mmmh
Stock Number:
039859-MU
Citation ID:
RRID:MMRRC_039859-MU
Other Names:
R1832 (G1), C57BL/6J-MtgxR1832Btlr
Major Collection:
Beutler Mutagenetix

Gene Information

Gene Details

Gene Symbol: Adamts20 [MGI:2660628] (Mus musculus (mouse))
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: bt, ADAMTS-20
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Erbb4 [MGI:104771] (Mus musculus (mouse))
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: Her4, ErbB4
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Eif3h [MGI:1915385] (Mus musculus (mouse))
Name: eukaryotic translation initiation factor 3, subunit H
Synonyms: EIF3-P40, EIF3-gamma, Eif3s3, 1110008A16Rik, 9430017H16Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Slc25a12 [MGI:1926080] (Mus musculus (mouse))
Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Synonyms: B230107K20Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Pdzd2 [MGI:1922394] (Mus musculus (mouse))
Name: PDZ domain containing 2
Synonyms: Pdzk3, A930022H17Rik, 4930537L06Rik, Gm21706, LOC223364
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Llgl2 [MGI:1918843] (Mus musculus (mouse))
Name: LLGL2 scribble cell polarity complex component
Synonyms: 9130006H11Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Wwp1 [MGI:1861728] (Mus musculus (mouse))
Name: WW domain containing E3 ubiquitin protein ligase 1
Synonyms: AIP5, SDRP1, Tiul1, 8030445B08Rik
Chromosome: 4
Alteration at locus: Chemically Induced
See All Alterations
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 10,204,890 bp
  • G to A, chromosome 1 at 38,813,276 bp
  • T to A, chromosome 1 at 68,346,534 bp
  • A to T, chromosome 1 at 83,022,747 bp
  • C to T, chromosome 1 at 153,166,187 bp
  • T to A, chromosome 1 at 158,857,316 bp
  • A to G, chromosome 1 at 180,694,731 bp
  • G to T, chromosome 2 at 11,767,400 bp
  • A to G, chromosome 2 at 25,685,139 bp
  • A to G, chromosome 2 at 27,235,569 bp
  • A to T, chromosome 2 at 59,001,899 bp
  • A to G, chromosome 2 at 71,333,710 bp
  • A to T, chromosome 2 at 128,762,212 bp
  • A to G, chromosome 2 at 143,793,269 bp
  • A to T, chromosome 2 at 144,254,822 bp
  • A to G, chromosome 2 at 145,861,171 bp
  • T to A, chromosome 2 at 150,848,418 bp
  • G to A, chromosome 3 at 18,054,975 bp
  • A to G, chromosome 3 at 41,727,111 bp
  • C to T, chromosome 3 at 106,014,596 bp
  • T to A, chromosome 3 at 124,556,860 bp
  • C to T, chromosome 4 at 13,835,628 bp
  • T to C, chromosome 4 at 19,650,197 bp
  • T to G, chromosome 4 at 59,066,441 bp
  • G to A, chromosome 4 at 115,569,731 bp
  • A to T, chromosome 4 at 123,325,092 bp
  • G to A, chromosome 4 at 130,077,057 bp
  • A to G, chromosome 4 at 134,934,592 bp
  • A to G, chromosome 4 at 145,538,210 bp
  • T to A, chromosome 4 at 154,002,546 bp
  • T to A, chromosome 5 at 72,121,938 bp
  • G to A, chromosome 5 at 73,607,426 bp
  • C to T, chromosome 5 at 135,073,740 bp
  • A to T, chromosome 5 at 137,031,299 bp
  • A to T, chromosome 5 at 143,967,179 bp
  • A to C, chromosome 6 at 32,197,826 bp
  • A to G, chromosome 6 at 43,174,900 bp
  • G to T, chromosome 6 at 66,731,796 bp
  • A to T, chromosome 6 at 69,617,293 bp
  • A to G, chromosome 6 at 83,403,645 bp
  • G to A, chromosome 6 at 111,246,161 bp
  • G to T, chromosome 7 at 26,312,210 bp
  • G to A, chromosome 7 at 34,208,236 bp
  • T to A, chromosome 7 at 83,794,735 bp
  • T to A, chromosome 7 at 114,016,785 bp
  • A to G, chromosome 8 at 11,214,644 bp
  • A to G, chromosome 9 at 19,567,196 bp
  • A to C, chromosome 9 at 54,460,949 bp
  • A to T, chromosome 9 at 65,504,489 bp
  • T to C, chromosome 9 at 98,399,468 bp
  • A to G, chromosome 10 at 20,099,560 bp
  • A to T, chromosome 10 at 41,488,236 bp
  • G to T, chromosome 10 at 80,060,605 bp
  • T to C, chromosome 11 at 30,977,568 bp
  • T to C, chromosome 11 at 69,856,928 bp
  • A to T, chromosome 11 at 73,984,493 bp
  • A to T, chromosome 11 at 110,071,451 bp
  • A to G, chromosome 11 at 115,446,793 bp
  • G to T, chromosome 11 at 115,851,100 bp
  • T to C, chromosome 12 at 55,757,967 bp
  • G to A, chromosome 12 at 78,715,506 bp
  • G to A, chromosome 12 at 80,953,672 bp
  • T to C, chromosome 12 at 88,178,448 bp
  • A to G, chromosome 12 at 98,234,240 bp
  • A to G, chromosome 12 at 110,614,059 bp
  • T to A, chromosome 12 at 116,207,743 bp
  • T to A, chromosome 13 at 67,367,363 bp
  • T to A, chromosome 13 at 73,692,950 bp
  • A to G, chromosome 15 at 12,390,048 bp
  • T to C, chromosome 15 at 51,865,436 bp
  • T to C, chromosome 15 at 74,762,766 bp
  • A to T, chromosome 15 at 94,286,344 bp
  • C to A, chromosome 16 at 36,968,856 bp
  • T to A, chromosome 16 at 88,562,858 bp
  • A to G, chromosome 17 at 28,297,110 bp
  • C to A, chromosome 17 at 35,439,699 bp
  • G to A, chromosome 19 at 6,853,532 bp
  • A to G, chromosome 19 at 33,168,495 bp
  • C to T, chromosome 19 at 36,114,978 bp
  • T to A, chromosome 19 at 44,999,017 bp
  • C to T, chromosome X at 48,543,572 bp
  • T to C, chromosome X at 72,998,659 bp
  • C to A, chromosome X at 96,155,623 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1832 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039859-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH. This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 9+, Google Chrome, Safari.
Site Map