Strain Name:
C57BL/6J-MtgxR1853Btlr/Mmmh
Stock Number:
039877-MU
Citation ID:
RRID:MMRRC_039877-MU
Other Names:
R1853 (G1), C57BL/6J-MtgxR1853Btlr
Major Collection:

Gene Information

Gene Symbol: Lamb1 [MGI:96743] (Mus musculus (mouse))
Name: laminin B1
Synonyms: D130003D08Rik, Lamb-1, C81607, Lamb1-1, C80098
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Nes [MGI:101784] (Mus musculus (mouse))
Name: nestin
Synonyms: ESTM46, Marc2, Ifaprc2, RC2
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Dap3 [MGI:1929538] (Mus musculus (mouse))
Name: death associated protein 3
Synonyms: DAP-3, 4921514D13Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Macf1 [MGI:108559] (Mus musculus (mouse))
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Xpnpep1 [MGI:2180003] (Mus musculus (mouse))
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Pdia3 [MGI:95834] (Mus musculus (mouse))
Name: protein disulfide isomerase associated 3
Synonyms: ERp60, Erp, ERp61, Plca, PDI-Q2, ERp57, Grp58, PDI
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Nr4a1 [MGI:1352454] (Mus musculus (mouse))
Name: nuclear receptor subfamily 4, group A, member 1
Synonyms: Hmr, NGFI-B, N10, Hbr1, Hbr-1, TIS1, Nur77, NP10, GFRP1, TR3, Gfrp
Chromosome: 15
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 15,818,938 bp
  • G to A, chromosome 1 at 36,365,470 bp
  • T to C, chromosome 1 at 52,901,335 bp
  • GAAAAA to GAAAAAA, chromosome 1 at 63,305,542 bp
  • A to G, chromosome 1 at 75,198,128 bp
  • T to C, chromosome 1 at 78,499,016 bp
  • C to T, chromosome 1 at 90,945,667 bp
  • T to A, chromosome 1 at 96,988,721 bp
  • C to A, chromosome 1 at 181,454,621 bp
  • A to G, chromosome 1 at 183,306,445 bp
  • A to T, chromosome 2 at 37,085,220 bp
  • T to C, chromosome 2 at 120,688,751 bp
  • C to T, chromosome 2 at 121,360,650 bp
  • T to C, chromosome 2 at 121,431,663 bp
  • C to A, chromosome 2 at 177,509,139 bp
  • A to T, chromosome 3 at 26,133,522 bp
  • A to G, chromosome 3 at 87,975,807 bp
  • A to T, chromosome 3 at 88,632,915 bp
  • A to T, chromosome 3 at 88,930,926 bp
  • A to T, chromosome 3 at 105,459,752 bp
  • A to G, chromosome 3 at 108,812,979 bp
  • A to T, chromosome 3 at 116,779,322 bp
  • A to G, chromosome 3 at 145,891,549 bp
  • G to T, chromosome 4 at 32,768,062 bp
  • T to C, chromosome 4 at 43,009,727 bp
  • G to A, chromosome 4 at 47,025,762 bp
  • T to C, chromosome 4 at 47,049,387 bp
  • T to C, chromosome 4 at 123,512,720 bp
  • C to T, chromosome 4 at 154,291,106 bp
  • T to A, chromosome 5 at 14,676,684 bp
  • A to T, chromosome 5 at 38,673,407 bp
  • A to G, chromosome 5 at 65,624,029 bp
  • C to T, chromosome 5 at 75,952,905 bp
  • T to G, chromosome 5 at 110,306,853 bp
  • G to T, chromosome 5 at 114,111,921 bp
  • A to T, chromosome 5 at 120,807,995 bp
  • G to T, chromosome 5 at 121,869,121 bp
  • T to G, chromosome 6 at 8,014,574 bp
  • T to C, chromosome 6 at 18,408,602 bp
  • T to A, chromosome 6 at 28,545,564 bp
  • A to G, chromosome 6 at 34,875,559 bp
  • A to T, chromosome 6 at 47,813,227 bp
  • A to T, chromosome 6 at 55,976,328 bp
  • A to G, chromosome 6 at 92,901,369 bp
  • T to A, chromosome 6 at 113,290,285 bp
  • A to T, chromosome 7 at 5,481,247 bp
  • C to T, chromosome 7 at 28,155,546 bp
  • A to G, chromosome 7 at 43,659,936 bp
  • A to T, chromosome 7 at 97,453,424 bp
  • G to A, chromosome 7 at 116,083,569 bp
  • A to G, chromosome 7 at 128,009,082 bp
  • T to C, chromosome 8 at 24,898,626 bp
  • G to A, chromosome 8 at 64,823,006 bp
  • A to T, chromosome 8 at 84,224,200 bp
  • T to A, chromosome 9 at 57,144,715 bp
  • A to G, chromosome 9 at 59,393,393 bp
  • T to C, chromosome 9 at 108,945,446 bp
  • T to C, chromosome 9 at 122,826,206 bp
  • C to T, chromosome 10 at 59,441,622 bp
  • T to C, chromosome 10 at 75,773,304 bp
  • T to A, chromosome 10 at 78,812,924 bp
  • G to A, chromosome 10 at 86,758,246 bp
  • C to A, chromosome 11 at 58,309,979 bp
  • T to C, chromosome 11 at 58,316,964 bp
  • T to C, chromosome 11 at 70,010,870 bp
  • A to C, chromosome 11 at 120,098,636 bp
  • A to T, chromosome 12 at 8,010,928 bp
  • T to G, chromosome 12 at 31,318,272 bp
  • T to A, chromosome 12 at 59,071,613 bp
  • T to A, chromosome 12 at 81,378,334 bp
  • T to C, chromosome 13 at 90,883,588 bp
  • C to T, chromosome 13 at 93,625,335 bp
  • A to T, chromosome 13 at 106,860,887 bp
  • C to T, chromosome 14 at 31,140,463 bp
  • T to A, chromosome 14 at 32,576,816 bp
  • A to G, chromosome 14 at 50,084,120 bp
  • T to A, chromosome 14 at 55,588,479 bp
  • G to A, chromosome 14 at 57,585,907 bp
  • T to C, chromosome 14 at 60,244,547 bp
  • T to C, chromosome 15 at 27,756,536 bp
  • C to T, chromosome 15 at 54,845,823 bp
  • G to A, chromosome 15 at 58,097,289 bp
  • T to C, chromosome 15 at 101,271,764 bp
  • A to C, chromosome 16 at 17,107,223 bp
  • A to G, chromosome 16 at 23,890,653 bp
  • C to T, chromosome 16 at 31,117,304 bp
  • G to A, chromosome 16 at 35,268,947 bp
  • T to C, chromosome 16 at 56,260,277 bp
  • C to T, chromosome 17 at 3,415,135 bp
  • T to A, chromosome 17 at 21,434,142 bp
  • A to T, chromosome 17 at 21,464,323 bp
  • G to A, chromosome 17 at 22,945,527 bp
  • T to A, chromosome 17 at 23,477,455 bp
  • A to G, chromosome 17 at 23,820,525 bp
  • A to T, chromosome 17 at 28,429,307 bp
  • C to T, chromosome 17 at 31,455,120 bp
  • A to G, chromosome 17 at 32,314,149 bp
  • A to G, chromosome 17 at 36,889,078 bp
  • G to A, chromosome 17 at 45,661,297 bp
  • T to C, chromosome 17 at 56,202,885 bp
  • A to T, chromosome 17 at 80,274,879 bp
  • C to A, chromosome 17 at 88,671,305 bp
  • A to T, chromosome 18 at 12,513,705 bp
  • A to C, chromosome 18 at 24,695,969 bp
  • G to T, chromosome 18 at 34,833,393 bp
  • A to T, chromosome 18 at 46,285,700 bp
  • T to A, chromosome 18 at 77,462,975 bp
  • T to A, chromosome 18 at 77,871,799 bp
  • T to A, chromosome 19 at 3,965,822 bp
  • T to C, chromosome 19 at 11,973,891 bp
  • T to A, chromosome 19 at 29,380,482 bp
  • C to T, chromosome 19 at 46,137,473 bp
  • T to C, chromosome 19 at 53,006,210 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1853 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039877-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.