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Strain Name:
C57BL/6J-MtgxR1854Btlr/Mmmh
Stock Number:
039878-MU
Citation ID:
RRID:MMRRC_039878-MU
Other Names:
R1854 (G1), C57BL/6J-MtgxR1854Btlr
Major Collection:

Strain Information

Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
St3gal5
Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Synonyms: ST3Gal V, mST3Gal V, GM3-specific sialytransferase, GM3 synthase, [a]2, 3S-T, Siat9
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20454
Homologene: 2893
Htr2a
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: 5-HT2A receptor, Htr-2, Htr2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 15558
VEGA: 14
HGNC: HGNC:5293
Homologene: 68073
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Ulk2
Name: unc-51 like kinase 2
Synonyms: Unc51.2, A830085I22Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 29869
Homologene: 5891
Tfpi
Name: tissue factor pathway inhibitor
Synonyms: A630013F22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21788
Homologene: 4579
Eri3
Name: exoribonuclease 3
Synonyms: PINT1, Prnpip1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 140546
Homologene: 15403
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 12,838,437 bp
  • GAAAAA to GAAAAAA, chromosome 1 at 63,305,542 bp
  • C to A, chromosome 1 at 66,631,414 bp
  • G to A, chromosome 1 at 72,586,099 bp
  • T to A, chromosome 1 at 84,728,145 bp
  • T to A, chromosome 1 at 96,988,721 bp
  • A to G, chromosome 1 at 105,554,498 bp
  • T to A, chromosome 1 at 119,476,067 bp
  • T to A, chromosome 1 at 135,827,730 bp
  • A to G, chromosome 1 at 153,249,872 bp
  • A to G, chromosome 1 at 166,165,190 bp
  • C to A, chromosome 1 at 181,454,621 bp
  • A to G, chromosome 2 at 21,369,124 bp
  • G to A, chromosome 2 at 24,397,456 bp
  • G to T, chromosome 2 at 26,344,713 bp
  • A to G, chromosome 2 at 35,122,684 bp
  • C to T, chromosome 2 at 36,421,874 bp
  • A to T, chromosome 2 at 65,363,516 bp
  • A to G, chromosome 2 at 76,751,429 bp
  • A to G, chromosome 2 at 80,446,380 bp
  • G to T, chromosome 2 at 82,993,257 bp
  • A to G, chromosome 2 at 84,458,107 bp
  • T to G, chromosome 2 at 107,296,484 bp
  • T to C, chromosome 2 at 121,431,663 bp
  • T to C, chromosome 2 at 128,675,890 bp
  • C to T, chromosome 2 at 164,771,362 bp
  • A to G, chromosome 2 at 165,353,291 bp
  • C to A, chromosome 2 at 178,283,147 bp
  • A to G, chromosome 3 at 59,260,772 bp
  • C to T, chromosome 3 at 94,878,849 bp
  • A to G, chromosome 3 at 95,580,006 bp
  • G to A, chromosome 3 at 145,459,140 bp
  • A to T, chromosome 3 at 154,621,475 bp
  • G to A, chromosome 4 at 11,601,669 bp
  • A to G, chromosome 4 at 57,945,218 bp
  • G to A, chromosome 4 at 117,649,365 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • T to A, chromosome 4 at 152,538,010 bp
  • C to T, chromosome 5 at 29,477,782 bp
  • A to G, chromosome 5 at 52,815,826 bp
  • A to G, chromosome 5 at 65,166,963 bp
  • C to T, chromosome 5 at 67,732,155 bp
  • C to T, chromosome 5 at 75,952,905 bp
  • A to T, chromosome 5 at 101,888,186 bp
  • A to G, chromosome 5 at 115,873,901 bp
  • A to T, chromosome 5 at 123,046,629 bp
  • G to A, chromosome 5 at 124,804,689 bp
  • G to T, chromosome 5 at 128,747,839 bp
  • A to T, chromosome 5 at 143,925,896 bp
  • A to T, chromosome 6 at 47,813,227 bp
  • T to A, chromosome 6 at 50,565,329 bp
  • A to T, chromosome 6 at 55,976,328 bp
  • T to C, chromosome 6 at 72,132,093 bp
  • A to G, chromosome 6 at 92,901,369 bp
  • A to C, chromosome 6 at 113,842,283 bp
  • T to C, chromosome 6 at 128,502,225 bp
  • T to A, chromosome 6 at 132,893,329 bp
  • T to A, chromosome 6 at 139,993,049 bp
  • C to A, chromosome 7 at 28,296,410 bp
  • T to G, chromosome 7 at 29,775,371 bp
  • A to G, chromosome 7 at 43,659,936 bp
  • T to A, chromosome 7 at 45,377,220 bp
  • T to A, chromosome 7 at 45,517,943 bp
  • T to A, chromosome 7 at 101,667,305 bp
  • A to G, chromosome 7 at 128,009,082 bp
  • A to G, chromosome 8 at 21,135,484 bp
  • T to C, chromosome 8 at 25,033,581 bp
  • T to C, chromosome 8 at 93,358,822 bp
  • C to A, chromosome 8 at 110,528,059 bp
  • T to C, chromosome 8 at 126,382,343 bp
  • T to C, chromosome 9 at 58,199,816 bp
  • T to C, chromosome 9 at 77,452,588 bp
  • C to T, chromosome 9 at 103,449,831 bp
  • G to A, chromosome 9 at 110,088,672 bp
  • C to T, chromosome 10 at 43,894,545 bp
  • A to T, chromosome 10 at 62,764,517 bp
  • T to A, chromosome 10 at 76,614,812 bp
  • T to G, chromosome 10 at 76,721,949 bp
  • T to A, chromosome 10 at 78,812,924 bp
  • G to A, chromosome 10 at 86,758,246 bp
  • A to T, chromosome 10 at 89,606,765 bp
  • G to A, chromosome 10 at 127,080,516 bp
  • C to T, chromosome 11 at 23,426,153 bp
  • A to G, chromosome 11 at 52,257,064 bp
  • G to A, chromosome 11 at 58,455,431 bp
  • A to G, chromosome 11 at 61,783,527 bp
  • A to G, chromosome 11 at 75,609,103 bp
  • G to T, chromosome 11 at 76,786,338 bp
  • T to C, chromosome 11 at 78,232,540 bp
  • A to G, chromosome 11 at 80,370,325 bp
  • G to A, chromosome 11 at 99,603,017 bp
  • A to G, chromosome 11 at 109,143,918 bp
  • A to C, chromosome 12 at 13,229,331 bp
  • A to G, chromosome 12 at 24,713,152 bp
  • A to G, chromosome 12 at 30,897,805 bp
  • T to G, chromosome 12 at 31,318,272 bp
  • T to A, chromosome 12 at 59,071,613 bp
  • A to C, chromosome 12 at 78,680,283 bp
  • T to A, chromosome 12 at 81,378,334 bp
  • G to A, chromosome 12 at 112,044,812 bp
  • A to T, chromosome 13 at 67,207,817 bp
  • T to A, chromosome 13 at 67,670,357 bp
  • A to T, chromosome 13 at 76,825,741 bp
  • T to C, chromosome 13 at 90,883,588 bp
  • A to G, chromosome 14 at 36,918,591 bp
  • A to T, chromosome 14 at 60,036,953 bp
  • A to T, chromosome 14 at 74,705,753 bp
  • C to T, chromosome 14 at 123,460,412 bp
  • C to T, chromosome 15 at 54,845,823 bp
  • G to A, chromosome 15 at 58,097,289 bp
  • T to A, chromosome 15 at 84,854,762 bp
  • A to T, chromosome 15 at 88,600,486 bp
  • C to T, chromosome 15 at 91,901,620 bp
  • T to C, chromosome 15 at 101,271,764 bp
  • C to T, chromosome 16 at 4,697,269 bp
  • G to A, chromosome 16 at 32,680,142 bp
  • G to A, chromosome 16 at 35,268,947 bp
  • C to T, chromosome 16 at 37,062,109 bp
  • T to C, chromosome 16 at 38,324,297 bp
  • C to T, chromosome 16 at 88,707,780 bp
  • T to A, chromosome 17 at 25,035,839 bp
  • T to A, chromosome 17 at 32,537,099 bp
  • A to G, chromosome 17 at 38,559,533 bp
  • G to T, chromosome 17 at 49,901,771 bp
  • G to A, chromosome 17 at 55,611,556 bp
  • T to C, chromosome 17 at 56,202,885 bp
  • G to A, chromosome 17 at 74,627,128 bp
  • T to C, chromosome 18 at 37,322,340 bp
  • G to T, chromosome 18 at 61,743,294 bp
  • T to A, chromosome 18 at 77,871,799 bp
  • A to G, chromosome 19 at 6,252,431 bp
  • A to T, chromosome 19 at 6,320,807 bp
  • C to T, chromosome 19 at 9,013,832 bp
  • T to A, chromosome X at 53,254,056 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1854 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039878-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.