Strain Name:
C57BL/6J-MtgxR1856Btlr/Mmmh
Stock Number:
039880-MU
Citation ID:
RRID:MMRRC_039880-MU
Other Names:
R1856 (G1), C57BL/6J-MtgxR1856Btlr
Major Collection:

Gene Information

Gene Symbol: Zdhhc17 [MGI:2445110] (Mus musculus (mouse))
Name: zinc finger, DHHC domain containing 17
Synonyms: Hip14, A230053P19Rik, D130071N24Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Slc1a2 [MGI:101931] (Mus musculus (mouse))
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms: GLT-1, 2900019G14Rik, 1700091C19Rik, GLT1, MGLT1, Eaat2
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: P2rx7 [MGI:1339957] (Mus musculus (mouse))
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7R, P2X(7), P2X7 receptor
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Macf1 [MGI:108559] (Mus musculus (mouse))
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Pdzd2 [MGI:1922394] (Mus musculus (mouse))
Name: PDZ domain containing 2
Synonyms: Pdzk3, A930022H17Rik, 4930537L06Rik, Gm21706, LOC223364
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Thbs3 [MGI:98739] (Mus musculus (mouse))
Name: thrombospondin 3
Synonyms: TSP3, Thbs-3
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Ptpn3 [MGI:105307] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, non-receptor type 3
Synonyms: 9530011I20Rik, PTPCL, PTP-H1
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 14,899,388 bp
  • A to T, chromosome 1 at 16,677,273 bp
  • A to T, chromosome 1 at 80,606,568 bp
  • A to T, chromosome 1 at 134,355,545 bp
  • T to C, chromosome 1 at 150,721,664 bp
  • A to G, chromosome 1 at 161,106,742 bp
  • A to G, chromosome 1 at 163,933,696 bp
  • A to G, chromosome 1 at 172,175,553 bp
  • A to G, chromosome 1 at 193,167,535 bp
  • T to C, chromosome 2 at 36,734,345 bp
  • A to C, chromosome 2 at 69,245,923 bp
  • T to C, chromosome 2 at 76,743,633 bp
  • G to A, chromosome 2 at 89,054,859 bp
  • C to A, chromosome 2 at 102,777,567 bp
  • T to G, chromosome 2 at 103,970,727 bp
  • G to T, chromosome 2 at 112,335,927 bp
  • C to A, chromosome 2 at 120,724,936 bp
  • A to T, chromosome 2 at 128,659,788 bp
  • G to T, chromosome 2 at 150,577,708 bp
  • T to A, chromosome 2 at 180,910,970 bp
  • G to T, chromosome 3 at 25,440,037 bp
  • T to A, chromosome 3 at 54,279,989 bp
  • T to C, chromosome 3 at 59,331,272 bp
  • A to T, chromosome 3 at 82,058,352 bp
  • A to T, chromosome 3 at 89,226,406 bp
  • A to C, chromosome 4 at 25,620,352 bp
  • G to A, chromosome 4 at 56,771,568 bp
  • T to A, chromosome 4 at 57,239,682 bp
  • A to T, chromosome 4 at 65,340,743 bp
  • T to C, chromosome 4 at 101,598,988 bp
  • T to C, chromosome 4 at 115,860,125 bp
  • T to A, chromosome 4 at 123,369,848 bp
  • C to G, chromosome 4 at 130,378,043 bp
  • C to T, chromosome 4 at 138,967,362 bp
  • T to A, chromosome 4 at 140,720,604 bp
  • C to T, chromosome 4 at 141,004,012 bp
  • T to A, chromosome 4 at 144,777,883 bp
  • T to C, chromosome 4 at 148,271,632 bp
  • T to A, chromosome 4 at 155,611,729 bp
  • G to T, chromosome 5 at 14,778,552 bp
  • T to A, chromosome 5 at 106,847,676 bp
  • T to A, chromosome 5 at 122,681,032 bp
  • A to G, chromosome 6 at 30,661,006 bp
  • A to T, chromosome 7 at 6,008,266 bp
  • A to G, chromosome 7 at 12,632,311 bp
  • A to G, chromosome 7 at 19,194,061 bp
  • A to G, chromosome 7 at 67,226,231 bp
  • T to A, chromosome 7 at 90,381,679 bp
  • T to C, chromosome 7 at 104,300,857 bp
  • T to G, chromosome 7 at 120,278,181 bp
  • T to C, chromosome 8 at 3,852,728 bp
  • T to A, chromosome 8 at 19,023,576 bp
  • C to T, chromosome 8 at 23,929,135 bp
  • A to G, chromosome 8 at 25,957,256 bp
  • A to G, chromosome 8 at 111,476,181 bp
  • G to A, chromosome 8 at 124,551,403 bp
  • G to A, chromosome 9 at 22,004,575 bp
  • A to T, chromosome 9 at 22,353,331 bp
  • T to C, chromosome 9 at 38,877,596 bp
  • T to C, chromosome 9 at 39,994,359 bp
  • A to T, chromosome 9 at 44,802,754 bp
  • A to T, chromosome 9 at 45,775,758 bp
  • T to C, chromosome 9 at 56,926,011 bp
  • C to T, chromosome 9 at 63,534,337 bp
  • T to C, chromosome 9 at 76,259,785 bp
  • A to T, chromosome 9 at 83,869,263 bp
  • T to C, chromosome 9 at 86,492,004 bp
  • G to A, chromosome 9 at 87,022,209 bp
  • A to C, chromosome 9 at 108,297,162 bp
  • G to A, chromosome 9 at 110,517,719 bp
  • T to A, chromosome 10 at 78,196,451 bp
  • C to T, chromosome 10 at 93,707,353 bp
  • G to T, chromosome 10 at 107,854,264 bp
  • A to G, chromosome 10 at 110,947,293 bp
  • A to G, chromosome 10 at 117,750,906 bp
  • A to T, chromosome 11 at 23,867,379 bp
  • A to G, chromosome 11 at 42,626,713 bp
  • A to T, chromosome 11 at 46,291,502 bp
  • T to A, chromosome 11 at 59,040,296 bp
  • G to A, chromosome 11 at 67,255,682 bp
  • T to A, chromosome 11 at 69,034,658 bp
  • C to T, chromosome 11 at 94,238,123 bp
  • T to A, chromosome 11 at 99,519,088 bp
  • T to C, chromosome 11 at 105,221,298 bp
  • T to C, chromosome 11 at 120,563,218 bp
  • T to G, chromosome 12 at 13,474,229 bp
  • T to A, chromosome 12 at 33,358,770 bp
  • A to T, chromosome 12 at 51,744,794 bp
  • G to T, chromosome 12 at 79,147,662 bp
  • T to C, chromosome 12 at 98,810,584 bp
  • G to A, chromosome 12 at 101,883,333 bp
  • G to A, chromosome 12 at 110,636,509 bp
  • A to T, chromosome 12 at 110,933,064 bp
  • G to A, chromosome 12 at 113,545,303 bp
  • C to T, chromosome 13 at 34,953,028 bp
  • G to A, chromosome 13 at 43,668,042 bp
  • A to T, chromosome 13 at 55,718,062 bp
  • A to T, chromosome 13 at 67,345,358 bp
  • A to G, chromosome 14 at 26,927,749 bp
  • A to G, chromosome 14 at 33,088,073 bp
  • A to G, chromosome 14 at 56,043,699 bp
  • T to C, chromosome 14 at 64,828,648 bp
  • C to T, chromosome 14 at 68,570,948 bp
  • G to A, chromosome 14 at 77,480,160 bp
  • T to A, chromosome 14 at 121,954,741 bp
  • C to A, chromosome 15 at 11,152,081 bp
  • T to C, chromosome 15 at 12,373,855 bp
  • T to A, chromosome 15 at 58,846,599 bp
  • T to C, chromosome 15 at 99,940,302 bp
  • T to A, chromosome 16 at 17,081,647 bp
  • T to A, chromosome 16 at 90,761,695 bp
  • T to C, chromosome 17 at 24,968,978 bp
  • C to A, chromosome 17 at 25,160,471 bp
  • A to T, chromosome 17 at 32,965,913 bp
  • T to A, chromosome 17 at 46,306,603 bp
  • T to C, chromosome 17 at 50,607,850 bp
  • T to A, chromosome 18 at 12,537,781 bp
  • G to A, chromosome 18 at 36,644,527 bp
  • C to T, chromosome 19 at 3,597,346 bp
  • C to A, chromosome 19 at 9,002,048 bp
  • A to T, chromosome 19 at 10,798,602 bp
  • G to T, chromosome 19 at 10,902,416 bp
  • A to G, chromosome 19 at 12,462,356 bp
  • A to G, chromosome 19 at 47,098,187 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1856 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039880-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.