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Strain Name:
C57BL/6J-MtgxR1860Btlr/Mmmh
Stock Number:
039883-MU
Citation ID:
RRID:MMRRC_039883-MU
Other Names:
R1860 (G1), C57BL/6J-MtgxR1860Btlr
Major Collection:

Strain Information

Prkaca
Name: protein kinase, cAMP dependent, catalytic, alpha
Synonyms: Cs, C alpha, PKA, Pkaca
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18747
HGNC: HGNC:9380
Homologene: 121574
Npc2
Name: NPC intracellular cholesterol transporter 2
Synonyms: HE1, 2700012J19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67963
VEGA: 12
Homologene: 4697
Derl2
Name: Der1-like domain family, member 2
Synonyms: CGI-101, F-lana, Flana, Derlin-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 116891
Homologene: 9356
Adgre1
Name: adhesion G protein-coupled receptor E1
Synonyms: F4/80, DD7A5-7, TM7LN3, EGF-TM7, Ly71, Emr1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13733
VEGA: 17
HGNC: HGNC:3336
Homologene: 1493
Grin3a
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: NMDAR-L, NR3A, A830097C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242443
Homologene: 128613
Ldb1
Name: LIM domain binding 1
Synonyms: CLIM2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16825
HGNC: HGNC:6532
Homologene: 2891
Arpp21
Name: cyclic AMP-regulated phosphoprotein, 21
Synonyms: ARPP-21, Tarpp, D9Bwg1012e, 0710001E13Rik, R3hdm3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74100
Homologene: 32306
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 64,734,994 bp
  • T to C, chromosome 1 at 69,570,502 bp
  • A to C, chromosome 1 at 158,601,945 bp
  • T to C, chromosome 1 at 160,710,140 bp
  • T to A, chromosome 1 at 181,763,960 bp
  • C to A, chromosome 2 at 14,328,579 bp
  • C to T, chromosome 2 at 66,317,982 bp
  • T to C, chromosome 2 at 72,234,720 bp
  • T to G, chromosome 2 at 88,729,330 bp
  • G to A, chromosome 2 at 90,003,146 bp
  • C to A, chromosome 2 at 90,282,158 bp
  • T to A, chromosome 2 at 118,373,178 bp
  • A to G, chromosome 2 at 118,818,889 bp
  • G to A, chromosome 2 at 126,756,040 bp
  • G to A, chromosome 3 at 32,517,021 bp
  • A to G, chromosome 3 at 131,111,641 bp
  • G to A, chromosome 3 at 138,453,778 bp
  • T to C, chromosome 4 at 49,665,309 bp
  • C to T, chromosome 4 at 82,323,680 bp
  • T to C, chromosome 4 at 116,805,229 bp
  • A to T, chromosome 4 at 118,223,932 bp
  • T to C, chromosome 4 at 119,353,063 bp
  • G to A, chromosome 4 at 134,124,838 bp
  • A to G, chromosome 5 at 109,736,232 bp
  • T to A, chromosome 5 at 123,879,029 bp
  • T to A, chromosome 5 at 138,126,630 bp
  • T to C, chromosome 5 at 142,970,063 bp
  • T to C, chromosome 6 at 3,520,279 bp
  • T to A, chromosome 6 at 18,268,289 bp
  • T to C, chromosome 6 at 39,071,453 bp
  • G to T, chromosome 6 at 128,675,827 bp
  • A to T, chromosome 7 at 29,009,552 bp
  • C to T, chromosome 7 at 30,038,691 bp
  • A to G, chromosome 7 at 79,594,999 bp
  • A to T, chromosome 7 at 85,615,574 bp
  • T to C, chromosome 7 at 104,587,905 bp
  • T to C, chromosome 7 at 105,704,205 bp
  • A to G, chromosome 7 at 107,934,390 bp
  • T to A, chromosome 7 at 120,534,763 bp
  • T to G, chromosome 7 at 122,568,201 bp
  • T to A, chromosome 7 at 126,409,641 bp
  • T to A, chromosome 7 at 131,444,229 bp
  • G to T, chromosome 7 at 139,090,299 bp
  • G to A, chromosome 7 at 140,336,219 bp
  • G to A, chromosome 8 at 13,123,565 bp
  • A to T, chromosome 8 at 22,580,499 bp
  • A to G, chromosome 8 at 43,569,541 bp
  • T to C, chromosome 8 at 46,637,913 bp
  • T to C, chromosome 8 at 47,990,102 bp
  • T to C, chromosome 8 at 83,981,223 bp
  • A to G, chromosome 8 at 105,240,071 bp
  • A to G, chromosome 8 at 105,334,806 bp
  • T to C, chromosome 8 at 122,495,750 bp
  • G to A, chromosome 9 at 19,641,984 bp
  • A to T, chromosome 9 at 24,646,288 bp
  • A to T, chromosome 9 at 38,658,365 bp
  • A to T, chromosome 9 at 58,020,873 bp
  • A to T, chromosome 9 at 112,149,323 bp
  • A to T, chromosome 10 at 56,123,537 bp
  • G to T, chromosome 10 at 81,594,329 bp
  • A to G, chromosome 10 at 116,993,273 bp
  • A to T, chromosome 10 at 121,547,171 bp
  • A to G, chromosome 10 at 128,246,114 bp
  • T to C, chromosome 10 at 129,388,086 bp
  • C to T, chromosome 10 at 130,479,886 bp
  • A to T, chromosome 11 at 50,923,542 bp
  • A to G, chromosome 11 at 71,018,343 bp
  • T to A, chromosome 11 at 96,138,874 bp
  • A to T, chromosome 11 at 105,397,078 bp
  • G to A, chromosome 12 at 4,748,314 bp
  • T to A, chromosome 12 at 21,296,732 bp
  • C to T, chromosome 12 at 26,450,617 bp
  • A to G, chromosome 12 at 31,465,846 bp
  • T to C, chromosome 12 at 72,105,926 bp
  • G to A, chromosome 12 at 83,979,728 bp
  • A to T, chromosome 12 at 84,765,394 bp
  • G to A, chromosome 13 at 47,049,190 bp
  • A to G, chromosome 13 at 56,083,204 bp
  • T to A, chromosome 14 at 31,222,906 bp
  • T to A, chromosome 14 at 43,783,739 bp
  • A to T, chromosome 14 at 50,281,391 bp
  • A to C, chromosome 14 at 51,920,605 bp
  • G to A, chromosome 14 at 77,111,407 bp
  • A to C, chromosome 14 at 120,479,044 bp
  • T to C, chromosome 15 at 12,914,071 bp
  • C to A, chromosome 15 at 41,869,034 bp
  • A to T, chromosome 15 at 47,659,192 bp
  • A to T, chromosome 15 at 58,096,718 bp
  • A to G, chromosome 15 at 77,017,584 bp
  • G to T, chromosome 16 at 4,087,736 bp
  • A to T, chromosome 16 at 87,416,343 bp
  • G to T, chromosome 17 at 18,049,497 bp
  • C to T, chromosome 17 at 18,947,386 bp
  • T to C, chromosome 17 at 35,135,643 bp
  • T to C, chromosome 17 at 57,441,363 bp
  • T to A, chromosome 18 at 44,872,956 bp
  • T to A, chromosome 19 at 13,365,341 bp
  • T to C, chromosome 19 at 23,359,153 bp
  • G to A, chromosome 19 at 42,762,449 bp
  • A to T, chromosome 19 at 46,034,192 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1860 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039883-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.