Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR1860Btlr/Mmmh
Stock Number:
039883-MU
Citation ID:
RRID:MMRRC_039883-MU
Other Names:
R1860 (G1), C57BL/6J-MtgxR1860Btlr
Major Collection:
Beutler Mutagenetix

Gene Information

Gene Details

Gene Symbol: Prkaca [MGI:97592] (Mus musculus (mouse))
Name: protein kinase, cAMP dependent, catalytic, alpha
Synonyms: PKA, Pkaca, C alpha, Cs
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Npc2 [MGI:1915213] (Mus musculus (mouse))
Name: NPC intracellular cholesterol transporter 2
Synonyms: HE1, 2700012J19Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Derl2 [MGI:2151483] (Mus musculus (mouse))
Name: Der1-like domain family, member 2
Synonyms: Derlin-2, Flana, F-lana, CGI-101
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Adgre1 [MGI:106912] (Mus musculus (mouse))
Name: adhesion G protein-coupled receptor E1
Synonyms: Ly71, DD7A5-7, EGF-TM7, TM7LN3, Emr1, F4/80
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Grin3a [MGI:1933206] (Mus musculus (mouse))
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: A830097C19Rik, NMDAR-L, NR3A
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Ldb1 [MGI:894762] (Mus musculus (mouse))
Name: LIM domain binding 1
Synonyms: CLIM2
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Arpp21 [MGI:107562] (Mus musculus (mouse))
Name: cyclic AMP-regulated phosphoprotein, 21
Synonyms: 0710001E13Rik, D9Bwg1012e, ARPP-21, Tarpp, R3hdm3
Chromosome: 9
Alteration at locus: Chemically Induced
See All Alterations
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 64,734,994 bp
  • T to C, chromosome 1 at 69,570,502 bp
  • A to C, chromosome 1 at 158,601,945 bp
  • T to C, chromosome 1 at 160,710,140 bp
  • T to A, chromosome 1 at 181,763,960 bp
  • C to A, chromosome 2 at 14,328,579 bp
  • C to T, chromosome 2 at 66,317,982 bp
  • T to C, chromosome 2 at 72,234,720 bp
  • T to G, chromosome 2 at 88,729,330 bp
  • G to A, chromosome 2 at 90,003,146 bp
  • C to A, chromosome 2 at 90,282,158 bp
  • T to A, chromosome 2 at 118,373,178 bp
  • A to G, chromosome 2 at 118,818,889 bp
  • G to A, chromosome 2 at 126,756,040 bp
  • G to A, chromosome 3 at 32,517,021 bp
  • A to G, chromosome 3 at 131,111,641 bp
  • G to A, chromosome 3 at 138,453,778 bp
  • T to C, chromosome 4 at 49,665,309 bp
  • C to T, chromosome 4 at 82,323,680 bp
  • T to C, chromosome 4 at 116,805,229 bp
  • A to T, chromosome 4 at 118,223,932 bp
  • T to C, chromosome 4 at 119,353,063 bp
  • G to A, chromosome 4 at 134,124,838 bp
  • A to G, chromosome 5 at 109,736,232 bp
  • T to A, chromosome 5 at 123,879,029 bp
  • T to A, chromosome 5 at 138,126,630 bp
  • T to C, chromosome 5 at 142,970,063 bp
  • T to C, chromosome 6 at 3,520,279 bp
  • T to A, chromosome 6 at 18,268,289 bp
  • T to C, chromosome 6 at 39,071,453 bp
  • G to T, chromosome 6 at 128,675,827 bp
  • A to T, chromosome 7 at 29,009,552 bp
  • C to T, chromosome 7 at 30,038,691 bp
  • A to G, chromosome 7 at 79,594,999 bp
  • A to T, chromosome 7 at 85,615,574 bp
  • T to C, chromosome 7 at 104,587,905 bp
  • T to C, chromosome 7 at 105,704,205 bp
  • A to G, chromosome 7 at 107,934,390 bp
  • T to A, chromosome 7 at 120,534,763 bp
  • T to G, chromosome 7 at 122,568,201 bp
  • T to A, chromosome 7 at 126,409,641 bp
  • T to A, chromosome 7 at 131,444,229 bp
  • G to T, chromosome 7 at 139,090,299 bp
  • G to A, chromosome 7 at 140,336,219 bp
  • G to A, chromosome 8 at 13,123,565 bp
  • A to T, chromosome 8 at 22,580,499 bp
  • A to G, chromosome 8 at 43,569,541 bp
  • T to C, chromosome 8 at 46,637,913 bp
  • T to C, chromosome 8 at 47,990,102 bp
  • T to C, chromosome 8 at 83,981,223 bp
  • A to G, chromosome 8 at 105,240,071 bp
  • A to G, chromosome 8 at 105,334,806 bp
  • T to C, chromosome 8 at 122,495,750 bp
  • G to A, chromosome 9 at 19,641,984 bp
  • A to T, chromosome 9 at 24,646,288 bp
  • A to T, chromosome 9 at 38,658,365 bp
  • A to T, chromosome 9 at 58,020,873 bp
  • A to T, chromosome 9 at 112,149,323 bp
  • A to T, chromosome 10 at 56,123,537 bp
  • G to T, chromosome 10 at 81,594,329 bp
  • A to G, chromosome 10 at 116,993,273 bp
  • A to T, chromosome 10 at 121,547,171 bp
  • A to G, chromosome 10 at 128,246,114 bp
  • T to C, chromosome 10 at 129,388,086 bp
  • C to T, chromosome 10 at 130,479,886 bp
  • A to T, chromosome 11 at 50,923,542 bp
  • A to G, chromosome 11 at 71,018,343 bp
  • T to A, chromosome 11 at 96,138,874 bp
  • A to T, chromosome 11 at 105,397,078 bp
  • G to A, chromosome 12 at 4,748,314 bp
  • T to A, chromosome 12 at 21,296,732 bp
  • C to T, chromosome 12 at 26,450,617 bp
  • A to G, chromosome 12 at 31,465,846 bp
  • T to C, chromosome 12 at 72,105,926 bp
  • G to A, chromosome 12 at 83,979,728 bp
  • A to T, chromosome 12 at 84,765,394 bp
  • G to A, chromosome 13 at 47,049,190 bp
  • A to G, chromosome 13 at 56,083,204 bp
  • T to A, chromosome 14 at 31,222,906 bp
  • T to A, chromosome 14 at 43,783,739 bp
  • A to T, chromosome 14 at 50,281,391 bp
  • A to C, chromosome 14 at 51,920,605 bp
  • G to A, chromosome 14 at 77,111,407 bp
  • A to C, chromosome 14 at 120,479,044 bp
  • T to C, chromosome 15 at 12,914,071 bp
  • C to A, chromosome 15 at 41,869,034 bp
  • A to T, chromosome 15 at 47,659,192 bp
  • A to T, chromosome 15 at 58,096,718 bp
  • A to G, chromosome 15 at 77,017,584 bp
  • G to T, chromosome 16 at 4,087,736 bp
  • A to T, chromosome 16 at 87,416,343 bp
  • G to T, chromosome 17 at 18,049,497 bp
  • C to T, chromosome 17 at 18,947,386 bp
  • T to C, chromosome 17 at 35,135,643 bp
  • T to C, chromosome 17 at 57,441,363 bp
  • T to A, chromosome 18 at 44,872,956 bp
  • T to A, chromosome 19 at 13,365,341 bp
  • T to C, chromosome 19 at 23,359,153 bp
  • G to A, chromosome 19 at 42,762,449 bp
  • A to T, chromosome 19 at 46,034,192 bp
Genotype Determination
ES Cell Line
N/A

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice

    Strain Origin

    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1860 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

    Appearance

    Coat Color
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

    Reproductive Statistics

    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039883-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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