Strain Name:
C57BL/6J-MtgxR1928Btlr/Mmmh
Stock Number:
039946-MU
Citation ID:
RRID:MMRRC_039946-MU
Other Names:
R1928 (G1), C57BL/6J-MtgxR1928Btlr
Major Collection:

Gene Information

Gene Symbol: Stx8 [MGI:1890156] (Mus musculus (mouse))
Name: syntaxin 8
Synonyms: 4930571E13Rik, 1110002H11Rik, 0610007H08Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Pofut2 [MGI:1916863] (Mus musculus (mouse))
Name: protein O-fucosyltransferase 2
Synonyms: FUT13, 2310011G23Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Vps33a [MGI:1924823] (Mus musculus (mouse))
Name: VPS33A CORVET/HOPS core subunit
Synonyms: 3830421M04Rik, bf
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Smco3 [MGI:2443451] (Mus musculus (mouse))
Name: single-pass membrane protein with coiled-coil domains 3
Synonyms: C030030A07Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Tnrc6b [MGI:2443730] (Mus musculus (mouse))
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, 2700090M07Rik, D230019K20Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Trib2 [MGI:2145021] (Mus musculus (mouse))
Name: tribbles pseudokinase 2
Synonyms: TRB2
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Anks3 [MGI:1919865] (Mus musculus (mouse))
Name: ankyrin repeat and sterile alpha motif domain containing 3
Synonyms: 2700067D09Rik
Chromosome: 16
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,081,300 bp
  • T to C, chromosome 1 at 24,451,754 bp
  • G to T, chromosome 1 at 72,874,025 bp
  • T to C, chromosome 1 at 88,095,929 bp
  • G to A, chromosome 1 at 93,006,610 bp
  • T to C, chromosome 1 at 172,957,314 bp
  • A to G, chromosome 1 at 173,729,645 bp
  • T to C, chromosome 1 at 176,828,970 bp
  • A to G, chromosome 1 at 185,424,297 bp
  • G to T, chromosome 2 at 69,867,557 bp
  • A to G, chromosome 2 at 76,725,044 bp
  • A to G, chromosome 2 at 132,251,897 bp
  • A to T, chromosome 2 at 160,968,000 bp
  • T to A, chromosome 2 at 164,032,254 bp
  • T to A, chromosome 3 at 19,661,882 bp
  • A to G, chromosome 3 at 46,446,631 bp
  • G to T, chromosome 3 at 54,728,845 bp
  • T to C, chromosome 3 at 55,247,521 bp
  • G to A, chromosome 3 at 85,688,531 bp
  • G to T, chromosome 3 at 89,217,760 bp
  • C to T, chromosome 3 at 105,967,302 bp
  • T to A, chromosome 3 at 109,506,422 bp
  • A to T, chromosome 4 at 4,069,926 bp
  • G to A, chromosome 4 at 32,601,812 bp
  • A to G, chromosome 4 at 48,390,095 bp
  • A to G, chromosome 4 at 101,782,730 bp
  • T to C, chromosome 4 at 116,816,658 bp
  • G to A, chromosome 4 at 124,722,093 bp
  • T to C, chromosome 5 at 64,345,300 bp
  • A to T, chromosome 5 at 81,757,973 bp
  • C to T, chromosome 5 at 92,444,266 bp
  • A to G, chromosome 5 at 110,327,778 bp
  • C to A, chromosome 5 at 112,761,417 bp
  • G to A, chromosome 5 at 123,558,621 bp
  • A to G, chromosome 5 at 124,514,750 bp
  • A to G, chromosome 5 at 146,539,608 bp
  • A to G, chromosome 6 at 40,411,714 bp
  • G to T, chromosome 6 at 57,844,092 bp
  • T to A, chromosome 6 at 67,423,735 bp
  • C to A, chromosome 6 at 83,199,184 bp
  • A to T, chromosome 6 at 119,952,923 bp
  • A to T, chromosome 6 at 123,267,161 bp
  • T to A, chromosome 6 at 123,331,630 bp
  • T to G, chromosome 6 at 132,626,601 bp
  • A to G, chromosome 6 at 136,044,046 bp
  • G to A, chromosome 6 at 136,831,847 bp
  • A to T, chromosome 6 at 143,013,230 bp
  • T to A, chromosome 7 at 24,141,138 bp
  • T to C, chromosome 7 at 43,529,879 bp
  • C to A, chromosome 7 at 46,742,432 bp
  • T to A, chromosome 7 at 50,701,195 bp
  • G to GACGGCGGCT, chromosome 7 at 97,579,909 bp
  • T to C, chromosome 7 at 104,307,124 bp
  • T to C, chromosome 7 at 121,910,447 bp
  • C to T, chromosome 7 at 141,068,148 bp
  • T to A, chromosome 8 at 3,486,947 bp
  • T to C, chromosome 8 at 9,770,217 bp
  • A to G, chromosome 8 at 12,663,988 bp
  • A to G, chromosome 8 at 69,073,812 bp
  • A to G, chromosome 8 at 85,011,253 bp
  • T to A, chromosome 8 at 106,193,488 bp
  • T to C, chromosome 8 at 110,502,947 bp
  • C to A, chromosome 8 at 111,688,692 bp
  • T to A, chromosome 9 at 8,903,629 bp
  • T to C, chromosome 9 at 20,301,058 bp
  • T to C, chromosome 9 at 21,007,552 bp
  • T to A, chromosome 9 at 40,209,787 bp
  • C to T, chromosome 9 at 40,306,469 bp
  • T to C, chromosome 9 at 48,326,614 bp
  • A to G, chromosome 9 at 86,423,003 bp
  • C to T, chromosome 10 at 24,909,812 bp
  • T to C, chromosome 10 at 39,921,462 bp
  • T to A, chromosome 10 at 77,260,808 bp
  • A to T, chromosome 10 at 92,972,573 bp
  • T to C, chromosome 10 at 128,409,313 bp
  • T to C, chromosome 11 at 68,109,280 bp
  • T to C, chromosome 11 at 69,817,464 bp
  • C to T, chromosome 11 at 101,283,852 bp
  • C to T, chromosome 11 at 102,153,396 bp
  • C to T, chromosome 11 at 106,790,728 bp
  • T to A, chromosome 11 at 115,470,016 bp
  • T to A, chromosome 12 at 15,815,453 bp
  • T to C, chromosome 12 at 28,653,089 bp
  • T to A, chromosome 12 at 57,332,419 bp
  • C to T, chromosome 12 at 79,239,970 bp
  • T to C, chromosome 12 at 112,015,971 bp
  • A to G, chromosome 13 at 12,601,759 bp
  • A to G, chromosome 13 at 46,701,026 bp
  • A to G, chromosome 13 at 46,812,745 bp
  • A to G, chromosome 13 at 59,816,734 bp
  • A to T, chromosome 13 at 81,520,786 bp
  • A to C, chromosome 13 at 99,430,946 bp
  • G to T, chromosome 13 at 116,308,417 bp
  • A to G, chromosome 14 at 50,681,415 bp
  • A to G, chromosome 14 at 59,281,867 bp
  • C to T, chromosome 14 at 77,798,848 bp
  • A to G, chromosome 14 at 96,346,789 bp
  • T to A, chromosome 15 at 33,687,007 bp
  • T to A, chromosome 15 at 80,880,723 bp
  • C to T, chromosome 15 at 85,862,063 bp
  • A to G, chromosome 15 at 97,750,033 bp
  • C to T, chromosome 16 at 4,946,054 bp
  • T to A, chromosome 16 at 21,222,295 bp
  • A to T, chromosome 16 at 32,750,532 bp
  • C to T, chromosome 16 at 88,051,353 bp
  • A to G, chromosome 17 at 3,168,077 bp
  • T to A, chromosome 17 at 5,990,267 bp
  • T to C, chromosome 17 at 19,811,767 bp
  • C to A, chromosome 17 at 20,502,809 bp
  • A to C, chromosome 17 at 21,819,335 bp
  • T to C, chromosome 17 at 22,341,084 bp
  • T to A, chromosome 17 at 26,974,638 bp
  • T to C, chromosome 17 at 32,397,353 bp
  • A to G, chromosome 17 at 86,995,058 bp
  • G to C, chromosome 18 at 12,213,378 bp
  • C to A, chromosome 18 at 21,651,786 bp
  • A to T, chromosome 18 at 37,266,180 bp
  • T to C, chromosome 18 at 44,405,571 bp
  • G to T, chromosome 19 at 12,623,609 bp
  • T to C, chromosome 19 at 34,878,881 bp
  • T to C, chromosome 19 at 36,612,319 bp
  • C to T, chromosome 19 at 36,845,668 bp
  • C to T, chromosome X at 106,433,522 bp
  • A to G, chromosome Y at 729,733 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1928 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039946-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.