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Strain Name:
C57BL/6J-MtgxR1953Btlr/Mmmh
Stock Number:
039967-MU
Citation ID:
RRID:MMRRC_039967-MU
Other Names:
R1953 (G1), C57BL/6J-MtgxR1953Btlr
Major Collection:

Strain Information

Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894
Mlst8
Name: MTOR associated protein, LST8 homolog (S. cerevisiae)
Synonyms: 0610033N12Rik, mLST8, Gbl
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56716
VEGA: 17
Homologene: 6833
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74996
Homologene: 9929
Dmd
Name: dystrophin, muscular dystrophy
Synonyms: Duchenne muscular dystrophy, mdx, pke, X-linked muscular dystrophy, Dp427, Dp71, dys
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 13405
HGNC: HGNC:2928
Homologene: 20856
Dscaml1
Name: DS cell adhesion molecule like 1
Synonyms: 4930435C18Rik, 4921507G06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114873
VEGA: 9
Homologene: 79549
Zranb3
Name: zinc finger, RAN-binding domain containing 3
Synonyms: 4933425L19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226409
Homologene: 32770
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 60,234,840 bp
  • A to T, chromosome 1 at 127,999,399 bp
  • A to G, chromosome 1 at 164,126,512 bp
  • T to C, chromosome 2 at 65,525,680 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • G to A, chromosome 2 at 88,973,880 bp
  • A to G, chromosome 2 at 89,943,923 bp
  • G to C, chromosome 2 at 120,035,334 bp
  • A to G, chromosome 2 at 129,011,929 bp
  • G to A, chromosome 2 at 145,928,200 bp
  • A to C, chromosome 2 at 154,311,314 bp
  • G to A, chromosome 2 at 180,190,747 bp
  • T to A, chromosome 3 at 25,436,300 bp
  • G to A, chromosome 3 at 96,287,070 bp
  • T to C, chromosome 4 at 101,910,115 bp
  • A to T, chromosome 4 at 108,250,550 bp
  • C to T, chromosome 4 at 117,265,986 bp
  • A to T, chromosome 4 at 118,472,790 bp
  • C to T, chromosome 4 at 136,227,456 bp
  • G to A, chromosome 4 at 150,616,837 bp
  • C to T, chromosome 4 at 155,773,114 bp
  • A to G, chromosome 5 at 3,630,038 bp
  • T to C, chromosome 5 at 52,839,688 bp
  • T to G, chromosome 5 at 87,587,671 bp
  • A to G, chromosome 5 at 124,782,268 bp
  • A to G, chromosome 5 at 137,735,032 bp
  • A to G, chromosome 6 at 34,884,388 bp
  • G to T, chromosome 6 at 49,446,478 bp
  • A to G, chromosome 6 at 70,892,554 bp
  • T to C, chromosome 6 at 87,588,593 bp
  • C to A, chromosome 6 at 92,024,630 bp
  • A to G, chromosome 6 at 134,710,267 bp
  • A to G, chromosome 6 at 136,704,293 bp
  • A to T, chromosome 6 at 140,042,568 bp
  • G to T, chromosome 7 at 6,305,088 bp
  • A to G, chromosome 7 at 11,669,804 bp
  • G to T, chromosome 7 at 28,288,047 bp
  • T to A, chromosome 7 at 44,841,398 bp
  • C to A, chromosome 7 at 44,862,602 bp
  • T to A, chromosome 7 at 56,206,038 bp
  • A to G, chromosome 7 at 83,640,412 bp
  • T to C, chromosome 7 at 108,925,118 bp
  • T to A, chromosome 7 at 112,092,876 bp
  • C to T, chromosome 7 at 120,361,432 bp
  • C to T, chromosome 7 at 143,476,247 bp
  • A to G, chromosome 8 at 85,521,384 bp
  • A to G, chromosome 8 at 85,831,231 bp
  • A to G, chromosome 8 at 92,359,810 bp
  • G to A, chromosome 8 at 105,138,097 bp
  • T to C, chromosome 8 at 110,221,919 bp
  • G to A, chromosome 8 at 120,015,296 bp
  • T to C, chromosome 9 at 44,337,444 bp
  • C to T, chromosome 9 at 45,670,224 bp
  • T to A, chromosome 9 at 59,949,796 bp
  • G to A, chromosome 9 at 60,614,987 bp
  • G to T, chromosome 9 at 73,114,614 bp
  • G to T, chromosome 9 at 96,024,221 bp
  • T to C, chromosome 9 at 108,843,182 bp
  • T to C, chromosome 10 at 50,845,630 bp
  • T to A, chromosome 10 at 80,038,554 bp
  • A to T, chromosome 10 at 85,098,307 bp
  • A to G, chromosome 11 at 7,078,991 bp
  • C to A, chromosome 11 at 68,594,518 bp
  • A to G, chromosome 11 at 76,117,216 bp
  • A to T, chromosome 11 at 80,377,946 bp
  • T to A, chromosome 11 at 102,458,183 bp
  • G to C, chromosome 11 at 118,408,783 bp
  • T to C, chromosome 13 at 45,520,837 bp
  • A to G, chromosome 13 at 58,011,208 bp
  • G to A, chromosome 13 at 61,251,509 bp
  • G to A, chromosome 13 at 63,842,181 bp
  • T to G, chromosome 13 at 110,353,806 bp
  • T to A, chromosome 14 at 52,450,887 bp
  • A to G, chromosome 14 at 121,255,482 bp
  • T to C, chromosome 15 at 18,966,911 bp
  • G to A, chromosome 16 at 4,179,449 bp
  • G to A, chromosome 16 at 11,399,783 bp
  • G to T, chromosome 16 at 38,827,913 bp
  • T to C, chromosome 17 at 18,062,860 bp
  • AT to ATT, chromosome 17 at 24,478,013 bp
  • G to A, chromosome 17 at 26,944,687 bp
  • T to A, chromosome 17 at 56,103,849 bp
  • A to T, chromosome 17 at 66,940,580 bp
  • A to G, chromosome 17 at 74,691,224 bp
  • A to G, chromosome 18 at 76,262,705 bp
  • G to A, chromosome 18 at 80,754,307 bp
  • G to A, chromosome 19 at 46,380,948 bp
  • A to G, chromosome X at 23,261,581 bp
  • T to A, chromosome X at 83,830,517 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1953 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039967-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.