MMRRC:039974-MU

A to G, chromosome 1 at 53,684,983 bp
A to T, chromosome 1 at 58,659,278 bp
A to T, chromosome 1 at 84,445,456 bp
C to A, chromosome 1 at 87,929,827 bp
T to A, chromosome 1 at 96,867,929 bp
T to C, chromosome 1 at 150,675,991 bp
T to A, chromosome 1 at 150,677,376 bp
G to A, chromosome 2 at 11,757,528 bp
A to T, chromosome 2 at 13,340,017 bp
C to T, chromosome 2 at 32,420,651 bp
T to G, chromosome 2 at 66,023,019 bp
T to C, chromosome 2 at 76,814,305 bp
T to C, chromosome 2 at 88,340,201 bp
A to G, chromosome 2 at 106,001,923 bp
A to C, chromosome 2 at 112,794,467 bp
T to C, chromosome 2 at 120,463,940 bp
A to G, chromosome 2 at 122,167,684 bp
A to G, chromosome 2 at 132,550,566 bp
A to G, chromosome 2 at 168,262,984 bp
T to A, chromosome 3 at 125,438,682 bp
T to C, chromosome 3 at 139,050,556 bp
C to T, chromosome 3 at 145,938,221 bp
T to C, chromosome 4 at 40,261,044 bp
A to G, chromosome 4 at 102,597,460 bp
G to A, chromosome 4 at 132,377,248 bp
A to T, chromosome 4 at 133,753,090 bp
CTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTC to CTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTC, chromosome 4 at 155,614,472 bp
A to T, chromosome 5 at 108,799,286 bp
C to A, chromosome 5 at 116,146,184 bp
G to A, chromosome 5 at 120,856,439 bp
G to A, chromosome 5 at 122,737,512 bp
A to G, chromosome 5 at 137,032,175 bp
A to T, chromosome 5 at 143,174,682 bp
C to T, chromosome 6 at 48,975,753 bp
A to T, chromosome 6 at 78,371,814 bp
T to C, chromosome 6 at 84,073,903 bp
T to A, chromosome 6 at 123,230,546 bp
T to A, chromosome 6 at 129,697,384 bp
A to T, chromosome 7 at 5,115,534 bp
T to C, chromosome 7 at 5,327,738 bp
A to T, chromosome 7 at 16,368,565 bp
G to A, chromosome 7 at 27,329,018 bp
T to A, chromosome 7 at 43,721,007 bp
T to A, chromosome 7 at 47,427,235 bp
T to A, chromosome 7 at 64,230,230 bp
C to A, chromosome 7 at 101,373,015 bp
A to T, chromosome 7 at 104,160,412 bp
T to C, chromosome 7 at 106,741,394 bp
A to T, chromosome 7 at 133,687,006 bp
A to T, chromosome 7 at 140,176,683 bp
A to G, chromosome 7 at 140,943,347 bp
T to C, chromosome 7 at 141,862,637 bp
T to C, chromosome 8 at 3,495,742 bp
C to A, chromosome 8 at 61,384,863 bp
T to A, chromosome 8 at 95,540,831 bp
T to G, chromosome 8 at 110,221,750 bp
T to G, chromosome 9 at 21,580,310 bp
A to G, chromosome 9 at 37,803,946 bp
T to A, chromosome 9 at 38,698,204 bp
T to A, chromosome 9 at 75,147,857 bp
C to T, chromosome 9 at 108,845,817 bp
G to T, chromosome 9 at 109,485,936 bp
A to T, chromosome 9 at 111,391,628 bp
G to A, chromosome 10 at 12,698,155 bp
T to C, chromosome 10 at 79,907,095 bp
A to G, chromosome 10 at 80,644,705 bp
C to T, chromosome 10 at 130,034,318 bp
T to C, chromosome 11 at 29,736,464 bp
G to T, chromosome 11 at 69,827,310 bp
A to G, chromosome 11 at 74,908,716 bp
A to G, chromosome 11 at 83,121,753 bp
T to C, chromosome 12 at 3,781,190 bp
G to A, chromosome 13 at 46,864,838 bp
T to C, chromosome 14 at 27,438,664 bp
C to T, chromosome 14 at 27,479,789 bp
A to G, chromosome 14 at 51,130,432 bp
T to G, chromosome 14 at 96,106,413 bp
T to A, chromosome 14 at 108,912,190 bp
T to C, chromosome 15 at 30,647,111 bp
T to C, chromosome 15 at 32,562,731 bp
T to A, chromosome 15 at 35,875,551 bp
T to A, chromosome 15 at 37,336,314 bp
G to A, chromosome 15 at 76,297,464 bp
T to C, chromosome 15 at 81,706,503 bp
A to G, chromosome 15 at 85,511,044 bp
G to A, chromosome 15 at 90,970,848 bp
A to G, chromosome 16 at 43,898,081 bp
A to G, chromosome 16 at 77,076,371 bp
T to C, chromosome 17 at 8,942,918 bp
A to G, chromosome 17 at 25,209,645 bp
A to G, chromosome 17 at 33,884,587 bp
A to C, chromosome 17 at 46,480,224 bp
A to T, chromosome 17 at 55,662,531 bp
T to C, chromosome 17 at 55,791,497 bp
A to T, chromosome 18 at 61,065,783 bp
C to A, chromosome 19 at 8,169,193 bp
G to A, chromosome 19 at 12,462,911 bp
A to T, chromosome 19 at 16,725,631 bp
T to C, chromosome X at 42,827,201 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain GQC Summary

The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.

Suggested Control Mice

Littermates of all relevant genotypes.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R1960 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
039974-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All