Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR2014Btlr/Mmmh
Stock Number:
040023-MU
Citation ID:
RRID:MMRRC_040023-MU
Other Names:
R2014 (G1), C57BL/6J-MtgxR2014Btlr
Major Collection:

Strain Information

Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428
Gulo
Name: gulonolactone (L-) oxidase
Synonyms: L-gulono-gamma-lactone oxidase, sfx
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268756
VEGA: 14
HGNC: HGNC:4695
Homologene: 6566
Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Rgs14
Name: regulator of G-protein signaling 14
Synonyms: Rap1/rap2 interacting protein
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 51791
HGNC: HGNC:9996
Homologene: 4735
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,579,444 bp
  • T to C, chromosome 1 at 66,416,136 bp
  • A to T, chromosome 1 at 84,760,866 bp
  • T to C, chromosome 1 at 92,885,141 bp
  • T to C, chromosome 1 at 96,867,711 bp
  • G to A, chromosome 1 at 133,003,904 bp
  • T to C, chromosome 1 at 136,148,282 bp
  • C to T, chromosome 1 at 156,705,941 bp
  • T to A, chromosome 2 at 25,563,572 bp
  • A to T, chromosome 2 at 28,665,637 bp
  • C to A, chromosome 2 at 49,741,423 bp
  • C to T, chromosome 2 at 72,438,260 bp
  • T to A, chromosome 2 at 76,755,296 bp
  • T to A, chromosome 2 at 85,430,352 bp
  • T to C, chromosome 2 at 88,412,044 bp
  • T to C, chromosome 2 at 103,981,062 bp
  • T to C, chromosome 2 at 121,434,820 bp
  • A to G, chromosome 3 at 19,987,434 bp
  • A to G, chromosome 3 at 83,032,757 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to G, chromosome 3 at 95,506,692 bp
  • A to G, chromosome 3 at 97,635,682 bp
  • C to T, chromosome 3 at 148,826,475 bp
  • A to G, chromosome 4 at 11,521,273 bp
  • A to G, chromosome 4 at 58,956,525 bp
  • A to T, chromosome 4 at 83,005,852 bp
  • A to C, chromosome 4 at 111,896,349 bp
  • T to C, chromosome 4 at 114,191,565 bp
  • A to C, chromosome 4 at 133,662,723 bp
  • A to T, chromosome 4 at 144,779,725 bp
  • A to G, chromosome 4 at 145,108,508 bp
  • G to T, chromosome 4 at 152,132,959 bp
  • T to A, chromosome 5 at 5,455,964 bp
  • T to A, chromosome 5 at 52,647,624 bp
  • A to G, chromosome 5 at 114,411,149 bp
  • G to A, chromosome 5 at 120,463,153 bp
  • G to A, chromosome 5 at 130,231,812 bp
  • C to A, chromosome 6 at 13,208,338 bp
  • A to G, chromosome 6 at 28,446,210 bp
  • C to T, chromosome 6 at 41,314,651 bp
  • A to G, chromosome 6 at 42,744,850 bp
  • T to A, chromosome 6 at 73,173,419 bp
  • T to C, chromosome 6 at 83,386,075 bp
  • T to C, chromosome 6 at 112,328,741 bp
  • T to C, chromosome 6 at 115,574,550 bp
  • G to T, chromosome 6 at 124,325,498 bp
  • T to C, chromosome 7 at 80,502,399 bp
  • T to A, chromosome 7 at 85,620,637 bp
  • T to A, chromosome 7 at 116,350,931 bp
  • C to A, chromosome 7 at 143,741,692 bp
  • A to T, chromosome 8 at 68,331,083 bp
  • A to G, chromosome 8 at 94,525,510 bp
  • C to T, chromosome 9 at 3,343,216 bp
  • T to C, chromosome 9 at 21,837,062 bp
  • T to A, chromosome 9 at 109,106,619 bp
  • G to A, chromosome 9 at 110,634,071 bp
  • C to A, chromosome 9 at 114,381,585 bp
  • A to G, chromosome 10 at 4,047,894 bp
  • A to T, chromosome 10 at 8,729,413 bp
  • A to T, chromosome 10 at 18,511,592 bp
  • A to G, chromosome 10 at 33,929,824 bp
  • A to T, chromosome 10 at 61,303,895 bp
  • G to A, chromosome 10 at 77,875,120 bp
  • T to C, chromosome 10 at 78,042,958 bp
  • T to C, chromosome 10 at 78,879,388 bp
  • A to G, chromosome 10 at 82,738,980 bp
  • T to G, chromosome 10 at 107,667,422 bp
  • A to T, chromosome 10 at 122,920,725 bp
  • C to T, chromosome 11 at 49,648,041 bp
  • A to G, chromosome 11 at 74,817,369 bp
  • A to G, chromosome 11 at 99,349,492 bp
  • T to C, chromosome 11 at 118,262,508 bp
  • T to C, chromosome 11 at 120,562,696 bp
  • T to C, chromosome 12 at 57,576,217 bp
  • G to T, chromosome 12 at 111,833,788 bp
  • T to G, chromosome 13 at 3,649,512 bp
  • T to C, chromosome 13 at 17,989,794 bp
  • T to C, chromosome 13 at 27,247,965 bp
  • C to A, chromosome 13 at 55,383,700 bp
  • C to A, chromosome 13 at 73,675,725 bp
  • T to C, chromosome 13 at 78,025,853 bp
  • T to C, chromosome 14 at 57,124,756 bp
  • T to A, chromosome 14 at 66,009,047 bp
  • T to C, chromosome 14 at 73,174,386 bp
  • T to C, chromosome 15 at 35,607,142 bp
  • C to T, chromosome 15 at 64,767,878 bp
  • T to C, chromosome 15 at 76,248,534 bp
  • C to T, chromosome 15 at 86,299,684 bp
  • T to C, chromosome 15 at 94,561,644 bp
  • C to T, chromosome 15 at 102,322,714 bp
  • A to T, chromosome 16 at 14,169,457 bp
  • A to G, chromosome 16 at 16,863,775 bp
  • A to G, chromosome 16 at 20,642,317 bp
  • G to A, chromosome 16 at 35,936,427 bp
  • A to G, chromosome 16 at 37,078,366 bp
  • A to G, chromosome 16 at 94,262,740 bp
  • C to T, chromosome 17 at 23,837,490 bp
  • A to G, chromosome 17 at 29,849,313 bp
  • C to A, chromosome 17 at 32,158,000 bp
  • A to G, chromosome 17 at 37,056,782 bp
  • T to C, chromosome 17 at 43,497,906 bp
  • A to T, chromosome 17 at 45,541,311 bp
  • T to A, chromosome 17 at 47,791,559 bp
  • T to G, chromosome 17 at 48,036,914 bp
  • T to A, chromosome 17 at 53,497,516 bp
  • T to A, chromosome 17 at 56,853,855 bp
  • T to C, chromosome 17 at 78,814,184 bp
  • T to C, chromosome 17 at 80,337,254 bp
  • T to C, chromosome 18 at 12,524,721 bp
  • T to C, chromosome 18 at 31,833,599 bp
  • A to G, chromosome 18 at 34,315,591 bp
  • A to T, chromosome 18 at 34,946,648 bp
  • T to C, chromosome 18 at 44,410,296 bp
  • T to C, chromosome 18 at 78,150,386 bp
  • T to C, chromosome 19 at 9,013,181 bp
  • T to C, chromosome 19 at 10,940,425 bp
  • T to G, chromosome 19 at 17,120,523 bp
  • T to C, chromosome 19 at 26,683,905 bp
  • G to A, chromosome 19 at 42,762,512 bp
  • A to G, chromosome X at 61,184,814 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2014 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040023-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.