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Strain Name:
C57BL/6J-MtgxR2059Btlr/Mmmh
Stock Number:
040064-MU
Citation ID:
RRID:MMRRC_040064-MU
Other Names:
R2059 (G1), C57BL/6J-MtgxR2059Btlr
Major Collection:

Strain Information

Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Galr2
Name: galanin receptor 2
Synonyms: mGalR, GalR2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14428
HGNC: HGNC:4133
Homologene: 2863
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Kdm5b
Name: lysine demethylase 5B
Synonyms: PLU-1, 2010009J12Rik, Plu1, Rb-Bp2, 2210016I17Rik, D1Ertd202e, Jarid1b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75605
Homologene: 48448
Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101772
Homologene: 75079
Pbsn
Name: probasin
Synonyms: PB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 54192
Homologene: 124466
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 10,188,752 bp
  • T to A, chromosome 1 at 78,416,951 bp
  • G to T, chromosome 1 at 133,726,537 bp
  • T to A, chromosome 1 at 134,613,214 bp
  • T to G, chromosome 1 at 188,381,549 bp
  • A to G, chromosome 2 at 35,316,025 bp
  • G to A, chromosome 2 at 71,249,853 bp
  • A to T, chromosome 2 at 120,369,292 bp
  • A to T, chromosome 3 at 7,573,915 bp
  • C to A, chromosome 3 at 17,785,713 bp
  • T to A, chromosome 3 at 38,891,170 bp
  • A to T, chromosome 3 at 90,031,376 bp
  • T to A, chromosome 3 at 152,660,637 bp
  • G to T, chromosome 4 at 120,698,002 bp
  • T to C, chromosome 4 at 129,725,721 bp
  • T to G, chromosome 4 at 130,260,785 bp
  • G to A, chromosome 5 at 30,199,491 bp
  • T to G, chromosome 5 at 30,933,645 bp
  • A to G, chromosome 5 at 72,316,051 bp
  • A to G, chromosome 5 at 87,535,033 bp
  • A to T, chromosome 5 at 92,683,784 bp
  • A to G, chromosome 5 at 100,059,219 bp
  • C to T, chromosome 5 at 109,942,496 bp
  • T to C, chromosome 6 at 22,986,323 bp
  • T to C, chromosome 6 at 28,745,207 bp
  • G to A, chromosome 6 at 41,032,381 bp
  • G to A, chromosome 6 at 48,836,333 bp
  • T to A, chromosome 6 at 66,612,202 bp
  • T to A, chromosome 6 at 125,139,860 bp
  • T to G, chromosome 6 at 136,609,348 bp
  • A to T, chromosome 7 at 24,003,834 bp
  • T to A, chromosome 7 at 26,557,362 bp
  • T to C, chromosome 7 at 46,699,456 bp
  • T to A, chromosome 7 at 56,163,897 bp
  • T to A, chromosome 7 at 64,376,751 bp
  • A to G, chromosome 7 at 79,931,793 bp
  • A to T, chromosome 7 at 86,664,383 bp
  • G to T, chromosome 7 at 92,126,531 bp
  • T to C, chromosome 7 at 100,455,493 bp
  • T to C, chromosome 7 at 100,549,333 bp
  • A to G, chromosome 7 at 104,348,191 bp
  • A to C, chromosome 7 at 104,861,171 bp
  • A to G, chromosome 7 at 120,758,949 bp
  • G to A, chromosome 7 at 131,106,170 bp
  • G to T, chromosome 7 at 135,410,677 bp
  • G to C, chromosome 7 at 144,611,390 bp
  • A to G, chromosome 8 at 22,939,305 bp
  • A to T, chromosome 8 at 46,807,345 bp
  • A to C, chromosome 8 at 83,399,978 bp
  • A to G, chromosome 8 at 111,297,495 bp
  • A to T, chromosome 9 at 53,667,156 bp
  • A to G, chromosome 9 at 65,704,434 bp
  • A to T, chromosome 9 at 67,860,833 bp
  • T to C, chromosome 9 at 75,665,716 bp
  • T to C, chromosome 9 at 106,241,290 bp
  • T to C, chromosome 9 at 110,885,438 bp
  • T to C, chromosome 9 at 123,061,770 bp
  • A to G, chromosome 10 at 8,207,566 bp
  • T to A, chromosome 10 at 28,566,603 bp
  • T to A, chromosome 10 at 56,115,944 bp
  • A to T, chromosome 10 at 92,839,575 bp
  • A to T, chromosome 10 at 92,942,979 bp
  • A to G, chromosome 10 at 120,038,698 bp
  • T to A, chromosome 10 at 128,113,578 bp
  • A to G, chromosome 10 at 128,137,518 bp
  • T to A, chromosome 11 at 49,444,451 bp
  • T to C, chromosome 11 at 79,556,723 bp
  • A to T, chromosome 11 at 100,787,332 bp
  • T to A, chromosome 11 at 108,519,261 bp
  • C to A, chromosome 11 at 113,854,332 bp
  • T to C, chromosome 11 at 116,282,939 bp
  • T to A, chromosome 12 at 57,737,693 bp
  • A to C, chromosome 12 at 65,138,806 bp
  • T to A, chromosome 12 at 85,139,623 bp
  • C to T, chromosome 12 at 87,443,750 bp
  • A to C, chromosome 13 at 13,500,473 bp
  • A to G, chromosome 13 at 27,270,144 bp
  • A to T, chromosome 13 at 33,156,871 bp
  • A to G, chromosome 13 at 38,023,380 bp
  • T to C, chromosome 13 at 41,109,954 bp
  • A to G, chromosome 13 at 110,260,516 bp
  • T to C, chromosome 14 at 26,668,880 bp
  • G to A, chromosome 14 at 32,332,884 bp
  • G to A, chromosome 14 at 56,563,955 bp
  • A to C, chromosome 14 at 60,759,591 bp
  • A to G, chromosome 14 at 70,585,049 bp
  • T to A, chromosome 15 at 21,583,740 bp
  • A to G, chromosome 15 at 102,984,123 bp
  • A to C, chromosome 16 at 36,122,322 bp
  • T to A, chromosome 16 at 38,187,909 bp
  • T to A, chromosome 16 at 97,454,179 bp
  • A to G, chromosome 17 at 31,848,797 bp
  • A to T, chromosome 17 at 34,622,844 bp
  • A to T, chromosome 17 at 34,648,575 bp
  • A to G, chromosome 17 at 43,428,586 bp
  • G to A, chromosome 17 at 46,397,024 bp
  • A to T, chromosome 18 at 12,528,333 bp
  • A to T, chromosome 18 at 77,254,763 bp
  • T to C, chromosome X at 77,847,976 bp
  • A to G, chromosome X at 101,080,767 bp
  • T to C, chromosome X at 134,095,244 bp
  • T to A, chromosome Y at 10,553,910 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2059 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040064-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.