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Strain Name:
C57BL/6J-MtgxR2068Btlr/Mmmh
Stock Number:
040073-MU
Citation ID:
RRID:MMRRC_040073-MU
Other Names:
R2068 (G1), C57BL/6J-MtgxR2068Btlr
Major Collection:

Strain Information

Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Itm2b
Name: integral membrane protein 2B
Synonyms: D14Sel6, Bri2, Bricd2b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16432
VEGA: 14
HGNC: HGNC:6174
Homologene: 7388
Lrrn3
Name: leucine rich repeat protein 3, neuronal
Synonyms: NLRR-3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16981
VEGA: 12
Homologene: 36315
Tjp2
Name: tight junction protein 2
Synonyms: ZO-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21873
VEGA: 19
Homologene: 3541
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 12,840,403 bp
  • G to A, chromosome 1 at 59,143,782 bp
  • A to G, chromosome 1 at 71,600,439 bp
  • A to T, chromosome 1 at 110,137,936 bp
  • A to G, chromosome 1 at 177,102,985 bp
  • G to A, chromosome 2 at 25,658,041 bp
  • T to C, chromosome 2 at 39,123,013 bp
  • T to A, chromosome 2 at 65,752,073 bp
  • C to T, chromosome 2 at 118,123,537 bp
  • T to C, chromosome 2 at 151,668,007 bp
  • G to A, chromosome 3 at 86,792,122 bp
  • G to A, chromosome 4 at 81,335,830 bp
  • A to G, chromosome 4 at 130,774,434 bp
  • T to C, chromosome 4 at 143,896,912 bp
  • A to T, chromosome 4 at 155,951,523 bp
  • C to T, chromosome 5 at 34,825,982 bp
  • T to C, chromosome 5 at 87,092,241 bp
  • G to T, chromosome 5 at 108,570,225 bp
  • A to C, chromosome 5 at 114,911,237 bp
  • C to T, chromosome 5 at 136,030,577 bp
  • T to C, chromosome 5 at 147,875,453 bp
  • A to G, chromosome 6 at 32,517,616 bp
  • G to A, chromosome 6 at 38,636,993 bp
  • A to G, chromosome 6 at 73,021,182 bp
  • T to C, chromosome 6 at 101,150,699 bp
  • A to T, chromosome 6 at 137,522,174 bp
  • A to T, chromosome 6 at 149,036,548 bp
  • T to C, chromosome 7 at 4,523,799 bp
  • A to G, chromosome 7 at 5,147,049 bp
  • T to C, chromosome 7 at 19,244,814 bp
  • T to C, chromosome 7 at 46,229,481 bp
  • G to T, chromosome 7 at 56,132,497 bp
  • A to C, chromosome 7 at 73,409,631 bp
  • T to A, chromosome 7 at 106,714,166 bp
  • T to G, chromosome 7 at 106,926,955 bp
  • T to C, chromosome 7 at 108,212,340 bp
  • A to T, chromosome 7 at 116,372,891 bp
  • T to A, chromosome 7 at 117,643,466 bp
  • C to T, chromosome 7 at 127,866,665 bp
  • A to G, chromosome 8 at 66,887,780 bp
  • A to G, chromosome 8 at 86,665,775 bp
  • A to G, chromosome 8 at 94,345,828 bp
  • T to A, chromosome 8 at 123,526,537 bp
  • C to A, chromosome 8 at 123,914,575 bp
  • T to C, chromosome 9 at 39,820,550 bp
  • A to G, chromosome 9 at 44,358,316 bp
  • A to T, chromosome 9 at 44,464,282 bp
  • G to A, chromosome 9 at 45,043,871 bp
  • T to G, chromosome 9 at 108,110,684 bp
  • T to A, chromosome 9 at 108,126,550 bp
  • A to C, chromosome 10 at 21,142,096 bp
  • A to T, chromosome 11 at 4,681,496 bp
  • T to A, chromosome 11 at 48,915,169 bp
  • T to C, chromosome 11 at 49,169,563 bp
  • T to A, chromosome 11 at 53,470,265 bp
  • A to G, chromosome 11 at 58,597,570 bp
  • G to A, chromosome 11 at 70,316,002 bp
  • T to C, chromosome 11 at 76,000,251 bp
  • T to C, chromosome 11 at 118,275,018 bp
  • T to C, chromosome 11 at 119,499,582 bp
  • A to T, chromosome 12 at 41,452,996 bp
  • A to T, chromosome 12 at 73,284,670 bp
  • A to T, chromosome 12 at 118,940,568 bp
  • A to T, chromosome 13 at 4,238,392 bp
  • T to A, chromosome 13 at 27,660,887 bp
  • G to A, chromosome 13 at 60,751,208 bp
  • C to T, chromosome 13 at 73,607,723 bp
  • A to T, chromosome 13 at 74,323,968 bp
  • T to A, chromosome 13 at 93,090,524 bp
  • G to T, chromosome 14 at 20,577,205 bp
  • A to G, chromosome 14 at 33,410,846 bp
  • A to G, chromosome 14 at 42,413,701 bp
  • GTTAGGCT to GT, chromosome 14 at 60,760,871 bp
  • T to C, chromosome 14 at 66,356,260 bp
  • T to C, chromosome 14 at 73,363,135 bp
  • A to G, chromosome 14 at 75,917,696 bp
  • A to T, chromosome 14 at 79,768,211 bp
  • T to A, chromosome 14 at 121,908,279 bp
  • G to T, chromosome 15 at 4,791,070 bp
  • T to C, chromosome 15 at 92,318,062 bp
  • T to C, chromosome 15 at 94,282,458 bp
  • A to T, chromosome 15 at 98,551,942 bp
  • T to C, chromosome 15 at 99,082,463 bp
  • A to G, chromosome 16 at 33,727,590 bp
  • A to G, chromosome 16 at 92,077,240 bp
  • G to A, chromosome 17 at 27,098,076 bp
  • T to C, chromosome 17 at 28,320,825 bp
  • A to G, chromosome 17 at 32,135,508 bp
  • C to A, chromosome 18 at 37,943,516 bp
  • T to C, chromosome 19 at 6,298,078 bp
  • A to T, chromosome 19 at 14,449,749 bp
  • C to A, chromosome 19 at 24,122,323 bp
  • T to A, chromosome X at 67,920,702 bp
  • T to A, chromosome X at 73,771,751 bp
  • A to G, chromosome X at 101,267,810 bp
  • A to C, chromosome X at 157,945,306 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2068 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040073-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.