Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR2078Btlr/Mmmh
Stock Number:
040083-MU
Citation ID:
RRID:MMRRC_040083-MU
Other Names:
R2078 (G1), C57BL/6J-MtgxR2078Btlr
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Tomm20
Name: translocase of outer mitochondrial membrane 20
Synonyms: TOM20, 1810060K07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67952
Homologene: 44649
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129
Tmem63b
Name: transmembrane protein 63b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224807
Homologene: 101682
Katna1
Name: katanin p60 (ATPase-containing) subunit A1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 23924
HGNC: HGNC:6216
Homologene: 56014
Dlgap4
Name: DLG associated protein 4
Synonyms: WBP16, SAP90/PSD-95-associated protein 4, PSD-95/SAP90 binding protein 4, DAP4, Sapap4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228836
Homologene: 8935
Sfswap
Name: splicing factor SWAP
Synonyms: 6330437E22Rik, 1190005N23Rik, Sfrs8
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231769
Homologene: 134548
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 44,206,502 bp
  • T to G, chromosome 1 at 67,157,806 bp
  • T to C, chromosome 1 at 67,195,265 bp
  • C to A, chromosome 1 at 75,172,136 bp
  • T to C, chromosome 1 at 81,191,981 bp
  • T to C, chromosome 1 at 82,741,921 bp
  • C to G, chromosome 1 at 170,908,081 bp
  • C to A, chromosome 1 at 172,563,289 bp
  • T to C, chromosome 1 at 173,761,586 bp
  • A to T, chromosome 2 at 65,330,384 bp
  • C to T, chromosome 2 at 128,794,458 bp
  • T to C, chromosome 2 at 156,762,826 bp
  • A to G, chromosome 3 at 38,889,673 bp
  • A to G, chromosome 3 at 61,003,697 bp
  • A to G, chromosome 3 at 63,701,943 bp
  • G to A, chromosome 3 at 92,945,451 bp
  • A to G, chromosome 3 at 103,961,523 bp
  • T to A, chromosome 4 at 104,986,250 bp
  • C to A, chromosome 4 at 154,019,431 bp
  • T to C, chromosome 5 at 69,541,035 bp
  • A to G, chromosome 5 at 72,203,573 bp
  • A to T, chromosome 5 at 108,124,605 bp
  • A to G, chromosome 5 at 117,380,803 bp
  • A to G, chromosome 5 at 129,516,107 bp
  • A to T, chromosome 5 at 139,426,133 bp
  • G to A, chromosome 6 at 29,709,971 bp
  • A to G, chromosome 6 at 33,910,587 bp
  • A to G, chromosome 6 at 55,678,969 bp
  • A to T, chromosome 6 at 119,338,116 bp
  • A to G, chromosome 6 at 125,170,127 bp
  • A to G, chromosome 7 at 4,337,786 bp
  • A to T, chromosome 7 at 30,360,454 bp
  • G to A, chromosome 7 at 51,897,325 bp
  • A to T, chromosome 7 at 104,859,393 bp
  • A to T, chromosome 8 at 83,003,605 bp
  • A to G, chromosome 8 at 112,015,403 bp
  • T to A, chromosome 8 at 124,611,491 bp
  • T to C, chromosome 8 at 126,937,072 bp
  • T to C, chromosome 9 at 44,707,979 bp
  • G to T, chromosome 9 at 45,968,829 bp
  • C to T, chromosome 10 at 7,743,569 bp
  • T to C, chromosome 10 at 67,243,952 bp
  • T to C, chromosome 10 at 99,263,824 bp
  • T to C, chromosome 10 at 106,967,041 bp
  • A to G, chromosome 11 at 121,245,394 bp
  • A to C, chromosome 12 at 51,748,542 bp
  • T to C, chromosome 13 at 114,990,045 bp
  • T to C, chromosome 14 at 7,927,466 bp
  • A to G, chromosome 14 at 14,052,975 bp
  • A to G, chromosome 14 at 60,291,987 bp
  • T to A, chromosome 14 at 78,908,157 bp
  • A to G, chromosome 15 at 44,527,767 bp
  • A to G, chromosome 15 at 44,636,804 bp
  • T to C, chromosome 15 at 77,763,912 bp
  • A to G, chromosome 17 at 6,782,642 bp
  • A to T, chromosome 17 at 34,568,715 bp
  • A to G, chromosome 17 at 45,663,536 bp
  • T to C, chromosome 17 at 64,714,591 bp
  • T to C, chromosome 18 at 63,117,720 bp
  • T to A, chromosome 19 at 12,862,387 bp
  • T to A, chromosome 19 at 21,400,672 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2078 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040083-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.