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Strain Name:
C57BL/6J-MtgxR2171Btlr/Mmmh
Stock Number:
040173-MU
Citation ID:
RRID:MMRRC_040173-MU
Other Names:
R2171 (G1), C57BL/6J-MtgxR2171Btlr
Major Collection:

Strain Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1C, Oct-1B, Oct-1A, oct-1, Otf-1, Otf1, 2810482H01Rik, Oct-1z, Oct1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091
Npy2r
Name: neuropeptide Y receptor Y2
Synonyms: NPY-Y2 receptor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18167
HGNC: HGNC:7957
Homologene: 701
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Vps54
Name: VPS54 GARP complex subunit
Synonyms: Vps54l, 5330404P15Rik, mSLP8, wr
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245944
Homologene: 5605
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: lectomedin 3, LEC3, D130075K09Rik, 5430402I23Rik, Lphn3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319387
Homologene: 22878
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 43,957,446 bp
  • T to C, chromosome 1 at 89,968,697 bp
  • T to C, chromosome 1 at 91,930,211 bp
  • A to G, chromosome 1 at 116,188,402 bp
  • A to T, chromosome 1 at 165,880,356 bp
  • A to G, chromosome 1 at 182,892,194 bp
  • T to A, chromosome 1 at 194,800,617 bp
  • T to A, chromosome 2 at 71,820,014 bp
  • G to A, chromosome 2 at 82,257,183 bp
  • G to A, chromosome 2 at 118,122,579 bp
  • A to T, chromosome 2 at 163,864,126 bp
  • A to T, chromosome 2 at 181,208,780 bp
  • C to T, chromosome 3 at 10,335,282 bp
  • T to C, chromosome 3 at 20,059,081 bp
  • T to C, chromosome 3 at 30,950,929 bp
  • T to G, chromosome 3 at 82,540,401 bp
  • C to T, chromosome 3 at 92,646,741 bp
  • A to G, chromosome 4 at 8,752,424 bp
  • T to C, chromosome 4 at 20,021,357 bp
  • T to C, chromosome 4 at 32,501,662 bp
  • T to A, chromosome 4 at 121,045,001 bp
  • G to C, chromosome 4 at 134,703,438 bp
  • T to C, chromosome 4 at 135,970,604 bp
  • A to T, chromosome 5 at 45,747,151 bp
  • G to A, chromosome 5 at 73,221,016 bp
  • C to A, chromosome 5 at 81,512,515 bp
  • A to G, chromosome 5 at 146,955,462 bp
  • A to T, chromosome 6 at 83,652,864 bp
  • A to G, chromosome 6 at 90,382,479 bp
  • C to T, chromosome 6 at 139,855,286 bp
  • T to G, chromosome 6 at 147,257,196 bp
  • T to C, chromosome 7 at 3,844,418 bp
  • A to G, chromosome 7 at 27,029,632 bp
  • C to T, chromosome 7 at 46,994,613 bp
  • T to C, chromosome 7 at 103,946,785 bp
  • T to G, chromosome 7 at 107,182,502 bp
  • T to A, chromosome 7 at 111,046,910 bp
  • T to C, chromosome 7 at 133,719,114 bp
  • T to A, chromosome 7 at 134,851,494 bp
  • T to G, chromosome 8 at 124,728,355 bp
  • T to A, chromosome 9 at 7,475,356 bp
  • T to G, chromosome 9 at 42,255,386 bp
  • T to C, chromosome 9 at 42,358,924 bp
  • A to T, chromosome 9 at 95,560,878 bp
  • T to C, chromosome 9 at 107,992,918 bp
  • T to C, chromosome 10 at 61,863,032 bp
  • A to C, chromosome 10 at 127,032,974 bp
  • A to G, chromosome 11 at 21,298,810 bp
  • A to G, chromosome 11 at 70,968,026 bp
  • A to T, chromosome 11 at 75,038,646 bp
  • A to G, chromosome 11 at 78,328,812 bp
  • A to G, chromosome 13 at 23,422,217 bp
  • A to G, chromosome 13 at 32,126,990 bp
  • A to G, chromosome 13 at 37,930,846 bp
  • A to T, chromosome 13 at 67,670,977 bp
  • A to G, chromosome 13 at 81,270,918 bp
  • C to T, chromosome 13 at 96,410,749 bp
  • A to G, chromosome 13 at 103,834,958 bp
  • GT to GTT, chromosome 14 at 31,315,051 bp
  • A to T, chromosome 14 at 50,736,419 bp
  • G to T, chromosome 15 at 35,887,197 bp
  • T to C, chromosome 15 at 101,800,910 bp
  • C to T, chromosome 17 at 75,291,317 bp
  • A to G, chromosome 18 at 21,026,086 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2171 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040173-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.