Strain Name:
C57BL/6J-MtgxR2215Btlr/Mmmh
Stock Number:
040217-MU
Citation ID:
RRID:MMRRC_040217-MU
Other Names:
R2215 (G1), C57BL/6J-MtgxR2215Btlr
Major Collection:

Strain Information

Fam117b
Name: family with sequence similarity 117, member B
Synonyms: 6330416D14Rik, 2810425F24Rik, Als2cr13
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72750
Homologene: 18307
Htr4
Name: 5 hydroxytryptamine (serotonin) receptor 4
Synonyms: 5-HT4L, 5-HT4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 15562
VEGA: 18
HGNC: HGNC:5299
Homologene: 20243
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Blm
Name: Bloom syndrome, RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12144
HGNC: HGNC:1058
Homologene: 47902
Actn4
Name: actinin alpha 4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68926
Homologene: 73649
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Plekhm1
Name: pleckstrin homology domain containing, family M (with RUN domain) member 1
Synonyms: D330036J23Rik, AP162, B2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 353047
Homologene: 8871
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: CD221, IGF-1R, line 186, hyft, A330103N21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Smarcc1
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
Synonyms: BAF155, SRG3, msp3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20588
Homologene: 68296
Fbxo31
Name: F-box protein 31
Synonyms: 2310046N15Rik, Fbx14, Fbxo14, 1110003O08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76454
Homologene: 11684
Eif4enif1
Name: eukaryotic translation initiation factor 4E nuclear import factor 1
Synonyms: Clast4, 2610509L04Rik, D11Ertd166e, A930019J01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74203
Homologene: 10522
Med25
Name: mediator complex subunit 25
Synonyms: ESTM2, 2610529E18Rik, 2610034E13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75613
Homologene: 12614
Txlna
Name: taxilin alpha
Synonyms: 2600010N21Rik, Txln, IL14
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109658
Homologene: 14062
Arap2
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Synonyms: Centd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 212285
Homologene: 9064
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: E130014J05Rik, Ayu17-449
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214133
Homologene: 49498
Bcas3
Name: BCAS3 microtubule associated cell migration factor
Synonyms: K20D4, 1500019F07Rik, 2610028P08Rik, rudhira, breast carcinoma amplified sequence 3, Phaf2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192197
Homologene: 9778
Akap8l
Name: A kinase anchor protein 8-like
Synonyms: Nakap95
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 54194
VEGA: 17
Homologene: 8658
Ppp2r1a
Name: protein phosphatase 2, regulatory subunit A, alpha
Synonyms: PR65, protein phosphatase PP2A, 6330556D22Rik, PP2A
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 51792
HGNC: HGNC:9302
Homologene: 68559
Rrp36
Name: ribosomal RNA processing 36
Synonyms: BC011248
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224823
VEGA: 17
Homologene: 40346
Cd72
Name: CD72 antigen
Synonyms: Ly-32, Ly-19, Ly-m19, Lyb-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12517
HGNC: HGNC:1696
Homologene: 1350
Gja8
Name: gap junction protein, alpha 8
Synonyms: Cnx50, connexin 50, alpha 8 connexin, Cx50, Aey5, Lop10, dcm
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14616
HGNC: HGNC:4281
Homologene: 3857
Acaca
Name: acetyl-Coenzyme A carboxylase alpha
Synonyms: acetyl-CoA carboxylase, Acc1, LOC327983, Acac, A530025K05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 107476
HGNC: HGNC:84
Homologene: 31015
Map3k1
Name: mitogen-activated protein kinase kinase kinase 1
Synonyms: MEKK1, Mekk
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26401
HGNC: HGNC:6848
Homologene: 8056
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 4833421P10Rik, A130030D10Rik, 1110059H15Rik, Zfp650
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68795
Homologene: 52092
Cep120
Name: centrosomal protein 120
Synonyms: Ccdc100
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225523
VEGA: 18
Homologene: 27415
Ppp3cb
Name: protein phosphatase 3, catalytic subunit, beta isoform
Synonyms: PP2BA beta, Calnb, CnAbeta, 1110063J16Rik, Cnab
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19056
HGNC: HGNC:9315
Homologene: 56429
D16Ertd472e
Name: DNA segment, Chr 16, ERATO Doi 472, expressed
Synonyms: 2310009O17Rik, E330003K22Rik, 1700010I10Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67102
Homologene: 9696
Nipsnap2
Name: nipsnap homolog 2
Synonyms: Nipsnap2, Gbas
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14467
HGNC: HGNC:4179
Homologene: 1137
Baz1a
Name: bromodomain adjacent to zinc finger domain 1A
Synonyms: Gtl5, Wcrf180, Acf1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217578
HGNC: HGNC:960
Homologene: 45654
Ddx20
Name: DEAD box helicase 20
Synonyms: GEMIN3, dp103, DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 53975
HGNC: HGNC:2743
Homologene: 5214
Retreg3
Name: reticulophagy regulator family member 3
Synonyms: 4933404C01Rik, 1300010M03Rik, Fam134c
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67998
Homologene: 23585
Usp6nl
Name: USP6 N-terminal like
Synonyms: TRE2NL
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98910
Homologene: 6879
Usp37
Name: ubiquitin specific peptidase 37
Synonyms: 4932415L06Rik, C330008N13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319651
Homologene: 10858
Kdm6b
Name: KDM1 lysine (K)-specific demethylase 6B
Synonyms: Jmjd3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216850
Homologene: 18945
Smox
Name: spermine oxidase
Synonyms: B130066H01Rik, SMO
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228608
Homologene: 69268
Peg10
Name: paternally expressed 10
Synonyms: MEF3L, HB-1, MyEF-3, MyEF-3 like, Edr, Mart2, Mar2, Rtl2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 170676
Homologene: 116067
Dach1
Name: dachshund family transcription factor 1
Synonyms: Dac, E130112M23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13134
HGNC: HGNC:2663
Homologene: 7288
Arhgef28
Name: Rho guanine nucleotide exchange factor 28
Synonyms: RIP2, RhoGEF, Rho specific exchange factor, D13Bwg1089e, 9230110L08Rik, p190RhoGEF, Rgnef
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110596
VEGA: 13
Homologene: 8078
Sppl2a
Name: signal peptide peptidase like 2A
Synonyms: C130089K23Rik, 2010106G01Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66552
Homologene: 36411
Tnfaip2
Name: tumor necrosis factor, alpha-induced protein 2
Synonyms: Tnfip2, Exoc3l3, M-sec
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21928
Homologene: 4583
Ttn
Name: titin
Synonyms: connectin, L56, 1100001C23Rik, mdm, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Pld1
Name: phospholipase D1
Synonyms: Pld1b, Pld1a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18805
HGNC: HGNC:9067
Homologene: 116234
Sec14l2
Name: SEC14-like lipid binding 2
Synonyms: 1300013M05Rik, tap, Spf
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67815
Homologene: 8245
Spats2l
Name: spermatogenesis associated, serine-rich 2-like
Synonyms: A230104H11Rik, 2810022L02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67198
Homologene: 56711
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 4833413O10Rik, D430029O09Rik, 1110021D17Rik, Polydom
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 64817
Homologene: 23386
Myh2
Name: myosin, heavy polypeptide 2, skeletal muscle, adult
Synonyms: MyHC-IIa, MHC2A, Myhs-f1, Myhs-f, Myhsf1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17882
HGNC: HGNC:7572
Homologene: 23019
Accs
Name: 1-aminocyclopropane-1-carboxylate synthase (inactive)
Synonyms: 2610203E10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329470
Homologene: 75335
Col14a1
Name: collagen, type XIV, alpha 1
Synonyms: 5730412L22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12818
HGNC: HGNC:2191
Homologene: 18741
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81877
Homologene: 49589
Gpatch1
Name: G patch domain containing 1
Synonyms: 1300003A17Rik, Gpatc1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67471
Homologene: 41217
Galntl5
Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5
Synonyms: 1700021B12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67909
Homologene: 12210
Sema5b
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Synonyms: SemG, SemG, Semag
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 20357
Homologene: 8427
Masp1
Name: MBL associated serine protease 1
Synonyms: Crarf
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17174
VEGA: 16
HGNC: HGNC:6901
Homologene: 88793
Pcdhb8
Name: protocadherin beta 8
Synonyms: Pcdhb5C, PcdhbH
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93879
HGNC: HGNC:8690
Homologene: 89038
Tgfb1i1
Name: transforming growth factor beta 1 induced transcript 1
Synonyms: TSC-5, hic-5, ARA55
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21804
Homologene: 7572
Map7d1
Name: MAP7 domain containing 1
Synonyms: Parcc1, Rprc1, Mtap7d1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 245877
Homologene: 17009
Mcam
Name: melanoma cell adhesion molecule
Synonyms: s-endo, CD146, s-gicerin, 1-gicerin, Muc18
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 84004
HGNC: HGNC:6934
Homologene: 4742
Zfp229
Name: zinc finger protein 229
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381067
Homologene: 136287
Mtmr10
Name: myotubularin related protein 10
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233315
Homologene: 9822
Adam21
Name: a disintegrin and metallopeptidase domain 21
Synonyms: ADAM31
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56622
HGNC: HGNC:200
Homologene: 68365
Vmn1r230
Name: vomeronasal 1 receptor 230
Synonyms: V1re8
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171231
Homologene: 136306
Lrrc34
Name: leucine rich repeat containing 34
Synonyms: 1700007J06Rik, Spata34
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71827
Homologene: 27419
Or4a68
Name: olfactory receptor family 4 subfamily A member 68
Synonyms: GA_x6K02T2Q125-50883183-50882239, MOR231-8, Olfr1240
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258804
Homologene: 121591
Bmp8a
Name: bone morphogenetic protein 8a
Synonyms: osteogenic protein 2, OP2, Bmp7r1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12163
Homologene: 135983
Or52n4b
Name: olfactory receptor family 52 subfamily N member 4B
Synonyms: GA_x6K02T2PBJ9-10874315-10875286, MOR34-9, MOR34-8P, MOR34-12, MOR34-9, Olfr1548, Olfr503
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259143
Homologene: 121509
Adrm1b
Name: adhesion regulating molecule 1B
Synonyms: Gm9774
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100043022
Cyp2a5
Name: cytochrome P450, family 2, subfamily a, polypeptide 5
Synonyms: Coh
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13087
Homologene: 85917
Gprin1
Name: G protein-regulated inducer of neurite outgrowth 1
Synonyms: Z16, GRIN1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26913
Homologene: 8072
Or6c3
Name: olfactory receptor family 6 subfamily C member 3
Synonyms: GA_x6K02T2PULF-11151514-11152449, MOR111-4, Olfr788
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258544
Homologene: 74200
Or9s27
Name: olfactory receptor family 9 subfamily S member 27
Synonyms: GA_x6K02T2R7CC-81165686-81164721, MOR208-4, Olfr1412
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258274
Homologene: 115514
Agmat
Name: agmatinase
Synonyms: 5033405N08Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 75986
Homologene: 99855
Lat
Name: linker for activation of T cells
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16797
Homologene: 7811
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 57,946,416 bp
  • T to A, chromosome 1 at 59,969,060 bp
  • A to C, chromosome 1 at 74,444,526 bp
  • G to A, chromosome 1 at 92,588,986 bp
  • A to G, chromosome 2 at 6,424,339 bp
  • A to T, chromosome 2 at 69,979,317 bp
  • T to C, chromosome 2 at 76,740,509 bp
  • T to C, chromosome 2 at 89,440,037 bp
  • G to T, chromosome 2 at 93,841,898 bp
  • G to T, chromosome 2 at 126,927,834 bp
  • T to C, chromosome 2 at 131,520,270 bp
  • A to T, chromosome 3 at 28,078,393 bp
  • G to A, chromosome 3 at 30,643,529 bp
  • G to A, chromosome 3 at 92,428,423 bp
  • A to T, chromosome 3 at 96,919,902 bp
  • A to T, chromosome 3 at 105,680,340 bp
  • T to A, chromosome 3 at 133,486,601 bp
  • T to C, chromosome 4 at 41,196,483 bp
  • T to C, chromosome 4 at 43,452,292 bp
  • T to C, chromosome 4 at 58,138,602 bp
  • C to T, chromosome 4 at 123,325,118 bp
  • C to T, chromosome 4 at 126,234,308 bp
  • T to C, chromosome 4 at 129,639,318 bp
  • C to T, chromosome 4 at 141,749,588 bp
  • A to T, chromosome 5 at 25,198,478 bp
  • T to C, chromosome 5 at 62,677,176 bp
  • A to T, chromosome 5 at 110,693,555 bp
  • A to G, chromosome 5 at 129,739,585 bp
  • A to T, chromosome 6 at 4,756,918 bp
  • T to C, chromosome 7 at 26,840,475 bp
  • C to A, chromosome 7 at 28,918,753 bp
  • A to T, chromosome 7 at 35,293,827 bp
  • T to C, chromosome 7 at 44,885,775 bp
  • A to G, chromosome 7 at 64,337,655 bp
  • T to A, chromosome 7 at 68,165,234 bp
  • A to T, chromosome 7 at 80,499,847 bp
  • T to C, chromosome 7 at 108,544,888 bp
  • A to T, chromosome 7 at 126,367,965 bp
  • G to A, chromosome 7 at 128,248,729 bp
  • T to C, chromosome 8 at 121,566,311 bp
  • G to T, chromosome 9 at 43,005,871 bp
  • A to T, chromosome 9 at 44,139,953 bp
  • C to A, chromosome 9 at 110,237,839 bp
  • A to T, chromosome 10 at 129,473,420 bp
  • A to G, chromosome 11 at 3,227,476 bp
  • C to A, chromosome 11 at 4,109,169 bp
  • T to C, chromosome 11 at 67,191,737 bp
  • G to A, chromosome 11 at 69,405,044 bp
  • T to A, chromosome 11 at 84,363,793 bp
  • T to C, chromosome 11 at 85,801,943 bp
  • G to T, chromosome 11 at 101,119,633 bp
  • A to T, chromosome 11 at 103,376,985 bp
  • G to A, chromosome 12 at 54,975,369 bp
  • A to G, chromosome 12 at 81,560,290 bp
  • T to C, chromosome 12 at 111,445,292 bp
  • G to A, chromosome 13 at 54,740,233 bp
  • G to T, chromosome 13 at 98,051,021 bp
  • A to G, chromosome 13 at 111,755,788 bp
  • A to T, chromosome 14 at 20,531,077 bp
  • A to G, chromosome 14 at 98,168,481 bp
  • G to A, chromosome 15 at 55,380,842 bp
  • T to A, chromosome 16 at 23,452,521 bp
  • A to G, chromosome 16 at 35,660,215 bp
  • T to C, chromosome 16 at 78,545,267 bp
  • T to C, chromosome 17 at 20,847,422 bp
  • A to G, chromosome 17 at 20,961,743 bp
  • T to G, chromosome 17 at 21,746,277 bp
  • T to C, chromosome 17 at 32,321,595 bp
  • T to C, chromosome 17 at 34,704,140 bp
  • T to C, chromosome 17 at 46,672,820 bp
  • T to G, chromosome 18 at 37,357,074 bp
  • G to T, chromosome 18 at 53,727,635 bp
  • T to A, chromosome 18 at 62,413,716 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2215 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040217-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.