Strain Name:
C57BL/6J-MtgxR2221Btlr/Mmmh
Stock Number:
040223-MU
Citation ID:
RRID:MMRRC_040223-MU
Other Names:
R2221 (G1), C57BL/6J-MtgxR2221Btlr
Major Collection:

Gene Information

Ntrk3
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18213
HGNC: HGNC:8033
Homologene: 49183
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242022
Homologene: 18454
Cradd
Name: CASP2 and RIPK1 domain containing adaptor with death domain
Synonyms: RAIDD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12905
VEGA: 10
HGNC: HGNC:2340
Homologene: 2821
Nup188
Name: nucleoporin 188
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227699
Homologene: 45683
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: EHSH1, Sh3p17, Ese1, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: CD221, IGF-1R, line 186, hyft, A330103N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Serpina9
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 9
Synonyms: Centerin, 2310014L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71907
Homologene: 23633
Cdk13
Name: cyclin-dependent kinase 13
Synonyms: 2310015O17Rik, Cdc2l5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 69562
VEGA: 13
HGNC: HGNC:1733
Homologene: 135707
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik, KMT8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Ttc39b
Name: tetratricopeptide repeat domain 39B
Synonyms: 9130422G05Rik, 1810054D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69863
Homologene: 25228
Setx
Name: senataxin
Synonyms: A930037J23Rik, Als4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269254
HGNC: HGNC:445
Homologene: 41003
Cry1
Name: cryptochrome 1 (photolyase-like)
Synonyms: Phll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12952
VEGA: 10
HGNC: HGNC:2384
Homologene: 7042
Eif3e
Name: eukaryotic translation initiation factor 3, subunit E
Synonyms: eIF3-p48, eIF3-p46, Int6, 48kDa, Eif3s6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16341
VEGA: 15
HGNC: HGNC:3277
Homologene: 1205
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D7H15F32S1, D15F32S1h, D7H15F37S1, rjs, jdf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Emsy
Name: EMSY, BRCA2-interacting transcriptional repressor
Synonyms: 2210018M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233545
Homologene: 32465
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, Afadin, S-afadin, I-afadin, 5033403D15Rik, Mllt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Tcof1
Name: treacle ribosome biogenesis factor 1
Synonyms: treacle
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 21453
Homologene: 68049
Bicd1
Name: BICD cargo adaptor 1
Synonyms: B830009D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12121
HGNC: HGNC:1049
Homologene: 37518
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Gas2
Name: growth arrest specific 2
Synonyms: Gas-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14453
HGNC: HGNC:4167
Homologene: 31301
Snap91
Name: synaptosomal-associated protein 91
Synonyms: F1-20, 91kDa, AP180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20616
Homologene: 8429
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, Coh1, C330002D13Rik, 2310042E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Adamtsl1
Name: ADAMTS-like 1
Synonyms: punctin-1, 6720426B09Rik, 5930437A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77739
Homologene: 64642
Trpa1
Name: transient receptor potential cation channel, subfamily A, member 1
Synonyms: ANKTM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 277328
HGNC: HGNC:497
Homologene: 7189
Mst1r
Name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Synonyms: STK, Fv-2, Ron, CDw136, friend virus susceptibility 2, PTK8, Fv2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19882
HGNC: HGNC:7381
Homologene: 1835
Aff2
Name: AF4/FMR2 family, member 2
Synonyms: Ox19, Oxh, Fmr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 14266
HGNC: HGNC:3776
Homologene: 1532
Col9a2
Name: collagen, type IX, alpha 2
Synonyms: Col9a-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12840
HGNC: HGNC:2218
Homologene: 37535
Hps3
Name: HPS3, biogenesis of lysosomal organelles complex 2 subunit 1
Synonyms: Hermansky-Pudlak syndrome 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12807
Homologene: 13019
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, D830007G01Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Map3k5
Name: mitogen-activated protein kinase kinase kinase 5
Synonyms: ASK1, Mekk5, 7420452D20Rik, ASK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 26408
HGNC: HGNC:6857
Homologene: 38114
Ctu2
Name: cytosolic thiouridylase subunit 2
Synonyms: 2310061F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66965
Homologene: 79815
Srgap3
Name: SLIT-ROBO Rho GTPase activating protein 3
Synonyms: Arhgap14, D130026O08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 259302
Homologene: 56686
Cdcp3
Name: CUB domain containing protein 3
Synonyms: 5430419D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71395
Homologene: 138430
Gbgt1
Name: globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227671
Homologene: 110677
Enkur
Name: enkurin, TRPC channel interacting protein
Synonyms: 4933434I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71233
Homologene: 17022
Dym
Name: dymeclin
Synonyms: C030019K18Rik, 1810041M12Rik, 4933427L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 69190
VEGA: 18
Homologene: 69237
Or4a71
Name: olfactory receptor family 4 subfamily A member 71
Synonyms: GA_x6K02T2Q125-50972538-50971621, MOR231-4, Olfr1243
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258971
Homologene: 27317
Zfp628
Name: zinc finger protein 628
Synonyms: Zec
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232816
Homologene: 72200
Or8b12i
Name: olfactory receptor family 8 subfamily B member 12I
Synonyms: GA_x6K02T2PVTD-13912679-13911744, MOR141-1, Olfr870
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 57251
Homologene: 138319
Slc19a1
Name: solute carrier family 19 (folate transporter), member 1
Synonyms: RFC1, RFC-1, reduced folate carrier
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20509
Homologene: 57139
Kcnc2
Name: potassium voltage gated channel, Shaw-related subfamily, member 2
Synonyms: Kv3.2, KShIIIA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 268345
VEGA: 10
HGNC: HGNC:6234
Homologene: 71199
Klri2
Name: killer cell lectin-like receptor family I member 2
Synonyms: A530090P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320407
Homologene: 86736
Vmn1r64
Name: vomeronasal 1 receptor 64
Synonyms: V1rd11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404285
Homologene: 41799
Gys2
Name: glycogen synthase 2
Synonyms: LGS, glycogen synthase, liver
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232493
HGNC: HGNC:4707
Homologene: 56580
Megf11
Name: multiple EGF-like-domains 11
Synonyms: 2410080H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214058
Homologene: 13031
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 628185
Homologene: 86604
Lrrc38
Name: leucine rich repeat containing 38
Synonyms: A230053A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242735
Homologene: 77949
Vmn1r25
Name: vomeronasal 1 receptor 25
Synonyms: V1rc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 113865
Homologene: 137652
Cfd
Name: complement factor D (adipsin)
Synonyms: D component (adipsin) of complement, DF, factor D, Adn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11537
VEGA: 10
HGNC: HGNC:2771
Homologene: 20453
Kif28
Name: kinesin family member 28
Synonyms: LOC383592, Gm1305
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 383592
Homologene: 130945
Fam184a
Name: family with sequence similarity 184, member A
Synonyms: 3110012E06Rik, 4930438C08Rik, 4930589M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 75906
Homologene: 11600
Ube2w
Name: ubiquitin-conjugating enzyme E2W (putative)
Synonyms: 6130401J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66799
Homologene: 10119
Aph1b
Name: aph1 homolog B, gamma secretase subunit
Synonyms: 2310057K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208117
Homologene: 12844
Vmn2r71
Name: vomeronasal 2, receptor 71
Synonyms: EG233445
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233445
Homologene: 115466
Cd1d2
Name: CD1d2 antigen
Synonyms: CD1.2, Cd1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12480
HGNC: HGNC:1637
Aspg
Name: asparaginase
Synonyms: A530050D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104816
VEGA: 12
Homologene: 113390
Gm5174
Name: predicted gene 5174
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382395
VEGA: 10
Homologene: 141143
Tex261
Name: testis expressed gene 261
Synonyms: TEG-261, 3110001O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21766
Homologene: 6131
Tbc1d30
Name: TBC1 domain family, member 30
Synonyms: 4930505D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74694
VEGA: 10
Homologene: 18930
Prl2a1
Name: prolactin family 2, subfamily a, member 1
Synonyms: PLP-M, Prlpm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 56635
HGNC: HGNC:9445
Homologene: 10556
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 14,903,256 bp
  • A to G, chromosome 1 at 16,597,959 bp
  • C to A, chromosome 1 at 179,733,111 bp
  • A to G, chromosome 2 at 21,189,319 bp
  • T to C, chromosome 2 at 28,498,423 bp
  • GTGGCT to GT, chromosome 2 at 29,154,061 bp
  • T to C, chromosome 2 at 30,336,924 bp
  • T to C, chromosome 2 at 76,742,094 bp
  • C to T, chromosome 2 at 89,527,937 bp
  • A to T, chromosome 3 at 20,002,363 bp
  • G to A, chromosome 3 at 53,516,857 bp
  • A to T, chromosome 3 at 86,988,540 bp
  • C to A, chromosome 4 at 32,763,306 bp
  • T to C, chromosome 4 at 83,232,762 bp
  • A to C, chromosome 4 at 86,388,525 bp
  • C to G, chromosome 4 at 121,054,258 bp
  • T to C, chromosome 4 at 143,134,899 bp
  • T to A, chromosome 4 at 143,369,849 bp
  • A to C, chromosome 6 at 57,979,238 bp
  • A to G, chromosome 6 at 83,771,515 bp
  • A to G, chromosome 6 at 112,946,493 bp
  • T to A, chromosome 6 at 129,740,309 bp
  • G to T, chromosome 6 at 142,456,422 bp
  • A to G, chromosome 6 at 149,517,005 bp
  • T to C, chromosome 7 at 4,920,831 bp
  • T to C, chromosome 7 at 5,884,449 bp
  • T to G, chromosome 7 at 51,993,278 bp
  • T to A, chromosome 7 at 56,169,018 bp
  • C to A, chromosome 7 at 68,201,962 bp
  • T to C, chromosome 7 at 78,198,852 bp
  • T to A, chromosome 7 at 85,624,093 bp
  • T to C, chromosome 7 at 98,590,775 bp
  • A to T, chromosome 7 at 131,247,457 bp
  • A to G, chromosome 8 at 122,480,910 bp
  • T to C, chromosome 9 at 20,171,092 bp
  • G to A, chromosome 9 at 64,660,431 bp
  • A to T, chromosome 9 at 66,784,639 bp
  • T to C, chromosome 9 at 86,792,527 bp
  • T to C, chromosome 9 at 107,908,348 bp
  • T to C, chromosome 10 at 20,067,920 bp
  • G to A, chromosome 10 at 53,655,079 bp
  • C to T, chromosome 10 at 77,042,486 bp
  • A to T, chromosome 10 at 79,892,205 bp
  • A to G, chromosome 10 at 85,143,753 bp
  • T to A, chromosome 10 at 86,656,508 bp
  • A to C, chromosome 10 at 95,175,873 bp
  • A to G, chromosome 10 at 112,456,526 bp
  • A to G, chromosome 10 at 121,294,577 bp
  • T to A, chromosome 12 at 103,998,264 bp
  • G to A, chromosome 12 at 112,114,434 bp
  • C to T, chromosome 13 at 11,605,144 bp
  • A to G, chromosome 13 at 17,719,535 bp
  • T to A, chromosome 13 at 27,806,386 bp
  • G to A, chromosome 15 at 35,884,597 bp
  • A to G, chromosome 15 at 43,251,547 bp
  • T to A, chromosome 16 at 91,853,768 bp
  • T to C, chromosome 17 at 13,883,737 bp
  • T to G, chromosome 17 at 22,601,233 bp
  • A to G, chromosome 18 at 60,837,901 bp
  • T to G, chromosome 18 at 75,230,165 bp
  • T to G, chromosome X at 69,694,001 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2221 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040223-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.