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Strain Name:
C57BL/6J-MtgxR2221Btlr/Mmmh
Stock Number:
040223-MU
Citation ID:
RRID:MMRRC_040223-MU
Other Names:
R2221 (G1), C57BL/6J-MtgxR2221Btlr
Major Collection:

Strain Information

Ntrk3
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18213
HGNC: HGNC:8033
Homologene: 49183
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Cradd
Name: CASP2 and RIPK1 domain containing adaptor with death domain
Synonyms: RAIDD
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12905
VEGA: 10
HGNC: HGNC:2340
Homologene: 2821
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Ese1, EHSH1, Sh3p17, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: CD221, IGF-1R, line 186, hyft, A330103N21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Serpina9
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 9
Synonyms: Centerin, 2310014L03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 71907
Homologene: 23633
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 14,903,256 bp
  • A to G, chromosome 1 at 16,597,959 bp
  • C to A, chromosome 1 at 179,733,111 bp
  • A to G, chromosome 2 at 21,189,319 bp
  • T to C, chromosome 2 at 28,498,423 bp
  • GTGGCT to GT, chromosome 2 at 29,154,061 bp
  • T to C, chromosome 2 at 30,336,924 bp
  • T to C, chromosome 2 at 76,742,094 bp
  • C to T, chromosome 2 at 89,527,937 bp
  • A to T, chromosome 3 at 20,002,363 bp
  • G to A, chromosome 3 at 53,516,857 bp
  • A to T, chromosome 3 at 86,988,540 bp
  • C to A, chromosome 4 at 32,763,306 bp
  • T to C, chromosome 4 at 83,232,762 bp
  • A to C, chromosome 4 at 86,388,525 bp
  • C to G, chromosome 4 at 121,054,258 bp
  • T to C, chromosome 4 at 143,134,899 bp
  • T to A, chromosome 4 at 143,369,849 bp
  • A to C, chromosome 6 at 57,979,238 bp
  • A to G, chromosome 6 at 83,771,515 bp
  • A to G, chromosome 6 at 112,946,493 bp
  • T to A, chromosome 6 at 129,740,309 bp
  • G to T, chromosome 6 at 142,456,422 bp
  • A to G, chromosome 6 at 149,517,005 bp
  • T to C, chromosome 7 at 4,920,831 bp
  • T to C, chromosome 7 at 5,884,449 bp
  • T to G, chromosome 7 at 51,993,278 bp
  • T to A, chromosome 7 at 56,169,018 bp
  • C to A, chromosome 7 at 68,201,962 bp
  • T to C, chromosome 7 at 78,198,852 bp
  • T to A, chromosome 7 at 85,624,093 bp
  • T to C, chromosome 7 at 98,590,775 bp
  • A to T, chromosome 7 at 131,247,457 bp
  • A to G, chromosome 8 at 122,480,910 bp
  • T to C, chromosome 9 at 20,171,092 bp
  • G to A, chromosome 9 at 64,660,431 bp
  • A to T, chromosome 9 at 66,784,639 bp
  • T to C, chromosome 9 at 86,792,527 bp
  • T to C, chromosome 9 at 107,908,348 bp
  • T to C, chromosome 10 at 20,067,920 bp
  • G to A, chromosome 10 at 53,655,079 bp
  • C to T, chromosome 10 at 77,042,486 bp
  • A to T, chromosome 10 at 79,892,205 bp
  • A to G, chromosome 10 at 85,143,753 bp
  • T to A, chromosome 10 at 86,656,508 bp
  • A to C, chromosome 10 at 95,175,873 bp
  • A to G, chromosome 10 at 112,456,526 bp
  • A to G, chromosome 10 at 121,294,577 bp
  • T to A, chromosome 12 at 103,998,264 bp
  • G to A, chromosome 12 at 112,114,434 bp
  • C to T, chromosome 13 at 11,605,144 bp
  • A to G, chromosome 13 at 17,719,535 bp
  • T to A, chromosome 13 at 27,806,386 bp
  • G to A, chromosome 15 at 35,884,597 bp
  • A to G, chromosome 15 at 43,251,547 bp
  • T to A, chromosome 16 at 91,853,768 bp
  • T to C, chromosome 17 at 13,883,737 bp
  • T to G, chromosome 17 at 22,601,233 bp
  • A to G, chromosome 18 at 60,837,901 bp
  • T to G, chromosome 18 at 75,230,165 bp
  • T to G, chromosome X at 69,694,001 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2221 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040223-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.