Strain Name:
C57BL/6J-MtgxR2227Btlr/Mmmh
Stock Number:
040228-MU
Citation ID:
RRID:MMRRC_040228-MU
Other Names:
R2227 (G1), C57BL/6J-MtgxR2227Btlr
Major Collection:

Gene Information

Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 268281
Homologene: 6489
Rc3h1
Name: RING CCCH (C3H) domains 1
Synonyms: roquin, 5730557L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381305
Homologene: 19036
Spns1
Name: spinster homolog 1
Synonyms: 2210013K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73658
Homologene: 41530
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 271639
Homologene: 10188
Cep55
Name: centrosomal protein 55
Synonyms: 2700032M20Rik, 1200008O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74107
VEGA: 19
HGNC: HGNC:1161
Homologene: 10019
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243725
Homologene: 14247
Fbxl20
Name: F-box and leucine-rich repeat protein 20
Synonyms: C86145, Fbl2, 2610511F20Rik, 4632423N09Rik, Scr, Scrapper
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72194
Homologene: 68784
Knl1
Name: kinetochore scaffold 1
Synonyms: 2310043D08Rik, 5730505K17Rik, Casc5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76464
Homologene: 44890
Cct2
Name: chaperonin containing Tcp1, subunit 2 (beta)
Synonyms: Cctb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12461
VEGA: 10
HGNC: HGNC:1615
Homologene: 4696
Exoc2
Name: exocyst complex component 2
Synonyms: Sec5, 2410030I24Rik, Sec5l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66482
Homologene: 10122
Kpna1
Name: karyopherin (importin) alpha 1
Synonyms: mSRP1, m-importin-alpha-S1, Rch2, NPI1, importin alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16646
HGNC: HGNC:6394
Homologene: 55642
Wdr3
Name: WD repeat domain 3
Synonyms: D030020G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 269470
Homologene: 4937
Gucy2c
Name: guanylate cyclase 2c
Synonyms: GC-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14917
HGNC: HGNC:4688
Homologene: 3641
Xpot
Name: exportin, tRNA (nuclear export receptor for tRNAs)
Synonyms: EXPORTIN-T, C79645, 1110004L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73192
VEGA: 10
Homologene: 38291
Il17rb
Name: interleukin 17 receptor B
Synonyms: Evi27, IL-17ER, IL17RH1, IL-17Rh1, Il17br
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50905
Homologene: 10287
Srsf6
Name: serine and arginine-rich splicing factor 6
Synonyms: 1210001E11Rik, Sfrs6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67996
Homologene: 110783
Marchf7
Name: membrane associated ring-CH-type finger 7
Synonyms: Axot, Gtrgeo17, March7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 57438
Homologene: 10753
Mtus1
Name: mitochondrial tumor suppressor 1
Synonyms: MD44, MTSG1, Atip1, B430305I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102103
Homologene: 100292
Ccl2
Name: chemokine (C-C motif) ligand 2
Synonyms: monocyte chemotactic protein, MCP-1, monocyte chemoattractant protein-1, Sigje, Scya2, MCP1, HC11, SMC-CF, MCAF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20296
Homologene: 2241
Ar
Name: androgen receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11835
HGNC: HGNC:644
Homologene: 28
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Miox
Name: myo-inositol oxygenase
Synonyms: RSOR, 0610009I10Rik, C85427, Aldrl6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 56727
Homologene: 9732
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Trappc11
Name: trafficking protein particle complex 11
Synonyms: D030016E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320714
Homologene: 11076
Agl
Name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Synonyms: 9430004C13Rik, 9630046L06Rik, 1110061O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77559
HGNC: HGNC:321
Homologene: 536
Rubcnl
Name: RUN and cysteine rich domain containing beclin 1 interacting protein like
Synonyms: LOC380917, 5031414D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 271221
VEGA: 14
Homologene: 57021
Gm6614
Name: predicted gene 6614
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 625716
Homologene: 87075
Tnn
Name: tenascin N
Synonyms: Tnw, tenascin-W
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329278
Homologene: 18634
Atg9b
Name: autophagy related 9B
Synonyms: LOC213948, Apg9l2, eONE, Nos3as
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 213948
Homologene: 72638
Myt1l
Name: myelin transcription factor 1-like
Synonyms: Png-1, Nztf1, Pmng1, C630034G21Rik, 2900093J19Rik, 2900046C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 17933
VEGA: 12
HGNC: HGNC:7623
Homologene: 7435
4930563I02Rik
Name: RIKEN cDNA 4930563I02 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67659
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225638
Homologene: 50475
Emcn
Name: endomucin
Synonyms: 0610012K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 59308
Homologene: 9438
Sgsm3
Name: small G protein signaling modulator 3
Synonyms: 1810012I01Rik, CIP85, Rutbc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105835
Homologene: 9249
Naalad2
Name: N-acetylated alpha-linked acidic dipeptidase 2
Synonyms: NAALADASE2, NAADALASE2, D9Ertd285e, GCPIII, GCP3, Folh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72560
Homologene: 3294
Zfp735
Name: zinc finger protein 735
Synonyms: 1700012C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76390
Homologene: 88945
Snx19
Name: sorting nexin 19
Synonyms: 3526401K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102607
VEGA: 9
Homologene: 8846
Ccdc33
Name: coiled-coil domain containing 33
Synonyms: LOC382077, 4930535E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382077
Homologene: 19641
Vmn1r86
Name: vomeronasal 1 receptor 86
Synonyms: Gm10301
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100312473
Homologene: 74345
Hdac9
Name: histone deacetylase 9
Synonyms: HDRP, Hdac7b, Mitr, D030072B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 79221
Homologene: 128578
Thoc2l
Name: THO complex subunit 2-like
Synonyms: Gm3179, BC005561
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100042165
Abtb1
Name: ankyrin repeat and BTB (POZ) domain containing 1
Synonyms: BPOZ, EF1ABP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 80283
Homologene: 32731
Cyp3a11
Name: cytochrome P450, family 3, subfamily a, polypeptide 11
Synonyms: IIIAm1, Cyp3a, Pcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13112
HGNC: HGNC:2638
Homologene: 133568
BB014433
Name: expressed sequence BB014433
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 434285
Krt6b
Name: keratin 6B
Synonyms: mK6[b], Krt2-6b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16688
VEGA: 15
Homologene: 136794
Clec2d
Name: C-type lectin domain family 2, member d
Synonyms: Clr-b, Clrb, Ocil
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 93694
Homologene: 136309
Lpar5
Name: lysophosphatidic acid receptor 5
Synonyms: LOC381810, GPR93, Gpr92, LPA5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 381810
Homologene: 10696
Tnnt2
Name: troponin T2, cardiac
Synonyms: Tnt, cTnT, cardiac TnT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21956
Homologene: 68050
Dgkk
Name: diacylglycerol kinase kappa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 331374
Homologene: 45867
Hoxa10
Name: homeobox A10
Synonyms: Hoxa-10, Hox-1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15395
HGNC: HGNC:5100
Homologene: 7365
Tex45
Name: testis expressed 45
Synonyms: 1700019B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76406
Homologene: 52367
Myl1
Name: myosin, light polypeptide 1
Synonyms: MLC3f, MLC1f, Mylf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17901
HGNC: HGNC:7582
Homologene: 23194
Tesl2
Name: testin LIM domain protein like 2
Synonyms: Gm4985
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245376
CU019598.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 66,944,815 bp
  • A to G, chromosome 1 at 135,843,791 bp
  • A to T, chromosome 1 at 160,147,465 bp
  • T to C, chromosome 1 at 160,963,542 bp
  • A to G, chromosome 1 at 165,518,260 bp
  • A to G, chromosome 2 at 60,229,846 bp
  • A to G, chromosome 2 at 119,072,000 bp
  • G to A, chromosome 2 at 162,931,699 bp
  • A to G, chromosome 3 at 100,146,791 bp
  • A to T, chromosome 3 at 116,788,312 bp
  • T to C, chromosome 3 at 137,404,017 bp
  • A to G, chromosome 5 at 24,386,395 bp
  • T to A, chromosome 5 at 104,519,420 bp
  • A to G, chromosome 5 at 145,868,547 bp
  • G to T, chromosome 6 at 5,154,074 bp
  • T to C, chromosome 6 at 52,232,636 bp
  • A to C, chromosome 6 at 88,836,367 bp
  • A to T, chromosome 6 at 125,081,135 bp
  • G to A, chromosome 6 at 129,184,251 bp
  • G to A, chromosome 6 at 136,702,760 bp
  • T to G, chromosome 6 at 141,992,361 bp
  • T to C, chromosome 7 at 13,102,920 bp
  • G to A, chromosome 7 at 126,371,165 bp
  • A to G, chromosome 8 at 3,479,249 bp
  • C to A, chromosome 8 at 15,041,717 bp
  • C to T, chromosome 8 at 41,082,775 bp
  • G to A, chromosome 8 at 47,524,957 bp
  • A to T, chromosome 9 at 18,376,533 bp
  • A to G, chromosome 9 at 30,429,260 bp
  • A to G, chromosome 9 at 58,082,022 bp
  • T to C, chromosome 10 at 11,162,235 bp
  • T to C, chromosome 10 at 117,053,017 bp
  • T to C, chromosome 10 at 121,622,860 bp
  • T to A, chromosome 11 at 73,711,396 bp
  • T to G, chromosome 11 at 73,711,397 bp
  • G to T, chromosome 11 at 82,036,601 bp
  • T to C, chromosome 11 at 98,090,849 bp
  • T to A, chromosome 12 at 29,826,970 bp
  • C to T, chromosome 12 at 34,407,802 bp
  • T to A, chromosome 13 at 30,864,884 bp
  • A to T, chromosome 14 at 30,006,081 bp
  • T to A, chromosome 14 at 60,088,219 bp
  • G to A, chromosome 14 at 75,042,392 bp
  • T to C, chromosome 15 at 44,512,792 bp
  • A to G, chromosome 15 at 81,003,868 bp
  • C to A, chromosome 15 at 89,335,662 bp
  • A to G, chromosome 15 at 101,679,122 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • G to A, chromosome 16 at 36,031,221 bp
  • T to A, chromosome 18 at 65,378,076 bp
  • T to C, chromosome 19 at 38,062,634 bp
  • T to A, chromosome X at 6,875,248 bp
  • T to A, chromosome X at 23,958,934 bp
  • A to T, chromosome X at 34,237,275 bp
  • T to A, chromosome X at 98,151,331 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2227 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040228-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.