Strain Name:
C57BL/6J-MtgxR2228Btlr/Mmmh
Stock Number:
040229-MU
Citation ID:
RRID:MMRRC_040229-MU
Other Names:
R2228 (G1), C57BL/6J-MtgxR2228Btlr
Major Collection:

Gene Information

Cadps
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 27062
VEGA: 14
HGNC: HGNC:1426
Homologene: 2755
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66860
Homologene: 18946
U2af2
Name: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22185
Homologene: 110853
Macroh2a1
Name: macroH2A.1 histone
Synonyms: H2AF12M, MACROH2A1.2, mH2a1, H2afy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26914
HGNC: HGNC:4740
Homologene: 3598
Pmepa1
Name: prostate transmembrane protein, androgen induced 1
Synonyms: N4wbp4, 2210418I02Rik, STAG1, PMEPA1, Tmepai
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 65112
Homologene: 10608
Bclaf1
Name: BCL2-associated transcription factor 1
Synonyms: 2610102K23Rik, 5730534O06Rik, 2700025J07Rik, 2810454G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 72567
Homologene: 8832
Wwp1
Name: WW domain containing E3 ubiquitin protein ligase 1
Synonyms: 8030445B08Rik, SDRP1, Tiul1, AIP5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 107568
Homologene: 21385
Eprs
Name: glutamyl-prolyl-tRNA synthetase
Synonyms: Qprs, 3010002K18Rik, 2410081F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 107508
HGNC: HGNC:3418
Homologene: 5870
Nup93
Name: nucleoporin 93
Synonyms: 2410008G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71805
Homologene: 40971
Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, Kiaa, b2b1752Clo, b2b1454Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Sipa1l3
Name: signal-induced proliferation-associated 1 like 3
Synonyms: 2610511M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74206
Homologene: 77938
Srbd1
Name: S1 RNA binding domain 1
Synonyms: D530025C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78586
VEGA: 17
Homologene: 9994
Ccdc18
Name: coiled-coil domain containing 18
Synonyms: 1700021E15Rik, 4932411G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73254
Homologene: 35455
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21819
Homologene: 2430
Taf2
Name: TATA-box binding protein associated factor 2
Synonyms: 4732460C16Rik, 150kDa, TAF2B, CIF150, TAFII150
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 319944
VEGA: 15
Homologene: 31137
Zfp512
Name: zinc finger protein 512
Synonyms: 2500002M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269639
Homologene: 13026
Golga1
Name: golgi autoantigen, golgin subfamily a, 1
Synonyms: 0710001G09Rik, 2210418B03Rik, golgin-97, Golgi97, awag
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76899
HGNC: HGNC:4424
Homologene: 68223
Myo9a
Name: myosin IXa
Synonyms: 4732465J09Rik, C130068I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270163
HGNC: HGNC:7608
Homologene: 21371
Nbn
Name: nibrin
Synonyms: Nbs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 27354
HGNC: HGNC:7652
Homologene: 1858
Atp6v1b2
Name: ATPase, H+ transporting, lysosomal V1 subunit B2
Synonyms: Atp6b2, HO57
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11966
HGNC: HGNC:854
Homologene: 1279
Flrt1
Name: fibronectin leucine rich transmembrane protein 1
Synonyms: D630040I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 396184
VEGA: 19
HGNC: HGNC:3760
Homologene: 40864
Cpsf2
Name: cleavage and polyadenylation specific factor 2
Synonyms: 100kDa, 2610024B04Rik, Cpsf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 51786
VEGA: 12
HGNC: HGNC:2325
Homologene: 6460
Htr2c
Name: 5-hydroxytryptamine (serotonin) receptor 2C
Synonyms: Htr1c, SR1, 5-HT2cR, 5-HT2C receptor, 5HT1c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 15560
HGNC: HGNC:5295
Homologene: 20242
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Dnahc8, Hst6.7b, P1-Loop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Acbd5
Name: acyl-Coenzyme A binding domain containing 5
Synonyms: 1300014E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74159
Homologene: 12541
Rufy1
Name: RUN and FYVE domain containing 1
Synonyms: 3000002E04Rik, Rabip4, ZFYVE12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216724
Homologene: 23522
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Tex15
Name: testis expressed gene 15
Synonyms: 2210014E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104271
Homologene: 12837
Myo16
Name: myosin XVI
Synonyms: Nyap3, BM140241, C230040D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244281
Homologene: 34710
Fstl5
Name: follistatin-like 5
Synonyms: 9130207J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 213262
Homologene: 10584
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Gm20114, Shn-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: Epcs21, GlcT-1, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Nckap1l
Name: NCK associated protein 1 like
Synonyms: Hem1, 4930568P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105855
VEGA: 15
HGNC: HGNC:4862
Homologene: 3901
Tro
Name: trophinin
Synonyms: trophinin-2, magphinin-gamma, magphinin-beta 2, Maged3l, Tnn, necdin and trophinin like, magphinin, Trol, magphinin-alpha, Maged3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 56191
Homologene: 75113
Pwwp2b
Name: PWWP domain containing 2B
Synonyms: D930023J19Rik, D7Ertd517e, Pwwp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101631
Homologene: 19056
Ptafr
Name: platelet-activating factor receptor
Synonyms: PAFR, PAF receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19204
HGNC: HGNC:9582
Homologene: 20260
Adcy8
Name: adenylate cyclase 8
Synonyms: AC8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11514
VEGA: 15
HGNC: HGNC:239
Homologene: 37443
Ifit1bl2
Name: interferon induced protein with tetratricopeptide repeats 1B like 2
Synonyms: 2010002M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 112419
Homologene: 78213
Ttc38
Name: tetratricopeptide repeat domain 38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239570
Homologene: 41208
Atp11b
Name: ATPase, class VI, type 11B
Synonyms: 1110019I14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76295
Homologene: 32919
Adamts16
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 271127
Homologene: 15146
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: LOC381522, E230008N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381522
Homologene: 117988
Fem1b
Name: fem 1 homolog b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 14155
VEGA: 9
HGNC: HGNC:3649
Homologene: 7714
Cep162
Name: centrosomal protein 162
Synonyms: 4922501C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382090
Homologene: 8930
Micall1
Name: microtubule associated monooxygenase, calponin and LIM domain containing -like 1
Synonyms: D15N2e, D15Mit260, Mus EST 820961
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 27008
VEGA: 15
Homologene: 24911
Ppp1r3c
Name: protein phosphatase 1, regulatory subunit 3C
Synonyms: PTG, protein targeting to glicogen, Ppp1r5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 53412
VEGA: 19
HGNC: HGNC:9293
Homologene: 3938
Samd9l
Name: sterile alpha motif domain containing 9-like
Synonyms: ESTM25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 209086
HGNC: HGNC:1349
Homologene: 7707
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: AI414108, LOC235086
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235086
Homologene: 19472
Disp3
Name: dispatched RND transporter family member 3
Synonyms: Ptchd2, G630052C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242748
Homologene: 25712
Adam24
Name: a disintegrin and metallopeptidase domain 24 (testase 1)
Synonyms: Dtgn5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 13526
HGNC: HGNC:203
Homologene: 137232
Aldh3b2
Name: aldehyde dehydrogenase 3 family, member B2
Synonyms: C130048D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 621603
HGNC: HGNC:411
Homologene: 84430
Spata18
Name: spermatogenesis associated 18
Synonyms: 1700067I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73472
Homologene: 17057
Vmn1r113
Name: vomeronasal 1 receptor 113
Synonyms: Gm5748
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 436135
Homologene: 104166
4932414N04Rik
Name: RIKEN cDNA 4932414N04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75721
Homologene: 138468
Mcm8
Name: minichromosome maintenance 8 homologous recombination repair factor
Synonyms: 5730432L01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66634
Homologene: 12001
Scel
Name: sciellin
Synonyms: 9230114I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 64929
VEGA: 14
Homologene: 2850
Sfxn4
Name: sideroflexin 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 94281
Homologene: 14228
Wdr35
Name: WD repeat domain 35
Synonyms: 4930459M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74682
Homologene: 10814
Ssx2ip
Name: synovial sarcoma, X 2 interacting protein
Synonyms: Adip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99167
Homologene: 8522
Olfr1350
Name: olfactory receptor 1350
Synonyms: GA_x6K02T2QGBW-3300391-3301317, MOR222-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258384
Ppp1r26
Name: protein phosphatase 1, regulatory subunit 26
Synonyms: LOC241289
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241289
Homologene: 49400
Entpd8
Name: ectonucleoside triphosphate diphosphohydrolase 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72090
Homologene: 77857
Ube2r2
Name: ubiquitin-conjugating enzyme E2R 2
Synonyms: Cdc34b, Ubc3b, 1200003M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67615
Homologene: 3210
Ac165356.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Ccdc153
Name: coiled-coil domain containing 153
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270150
VEGA: 9
Homologene: 26818
Tnfrsf22
Name: tumor necrosis factor receptor superfamily, member 22
Synonyms: 2810028K06Rik, mDcTrailr2, SOBa, C130035G06Rik, Tnfrh2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 79202
Homologene: 84848
Araf
Name: Araf proto-oncogene, serine/threonine kinase
Synonyms: A-Raf, 1200013E08Rik, Araf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11836
HGNC: HGNC:646
Homologene: 1249
Sppl2b
Name: signal peptide peptidase like 2B
Synonyms: 3110056O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73218
VEGA: 10
Homologene: 10605
Smim23
Name: small integral membrane protein 23
Synonyms: 1700008A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69351
Homologene: 85224
Zdhhc4
Name: zinc finger, DHHC domain containing 4
Synonyms: 2900029I10Rik, 1810021D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72881
Homologene: 10006
AC120128.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
P2ry4
Name: pyrimidinergic receptor P2Y, G-protein coupled, 4
Synonyms: P2Y4R, P2Y4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 57385
HGNC: HGNC:8542
Homologene: 20568
Capn6
Name: calpain 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 12338
HGNC: HGNC:1483
Homologene: 7252
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 185,367,537 bp
  • T to C, chromosome 2 at 23,089,592 bp
  • T to C, chromosome 2 at 25,085,016 bp
  • T to C, chromosome 2 at 28,453,786 bp
  • T to C, chromosome 2 at 39,023,171 bp
  • T to C, chromosome 2 at 52,232,995 bp
  • T to C, chromosome 2 at 59,724,724 bp
  • A to G, chromosome 2 at 68,729,591 bp
  • T to A, chromosome 2 at 132,820,121 bp
  • G to A, chromosome 2 at 173,228,133 bp
  • G to T, chromosome 3 at 35,806,942 bp
  • C to A, chromosome 3 at 76,482,352 bp
  • C to T, chromosome 3 at 146,417,776 bp
  • A to G, chromosome 4 at 15,970,904 bp
  • A to T, chromosome 4 at 19,641,745 bp
  • C to T, chromosome 4 at 41,174,044 bp
  • G to T, chromosome 4 at 45,948,856 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • T to G, chromosome 4 at 132,579,380 bp
  • A to T, chromosome 4 at 148,242,916 bp
  • A to G, chromosome 5 at 21,987,078 bp
  • A to T, chromosome 5 at 31,465,575 bp
  • A to G, chromosome 5 at 52,582,860 bp
  • A to T, chromosome 5 at 73,666,901 bp
  • T to A, chromosome 5 at 108,193,852 bp
  • A to C, chromosome 5 at 143,320,407 bp
  • T to C, chromosome 6 at 3,376,910 bp
  • T to C, chromosome 7 at 5,075,673 bp
  • A to T, chromosome 7 at 6,570,803 bp
  • C to T, chromosome 7 at 20,787,907 bp
  • T to A, chromosome 7 at 29,377,939 bp
  • T to G, chromosome 7 at 106,082,383 bp
  • T to C, chromosome 7 at 139,255,188 bp
  • C to T, chromosome 7 at 143,644,776 bp
  • G to A, chromosome 8 at 10,594,905 bp
  • C to A, chromosome 8 at 33,571,237 bp
  • A to T, chromosome 8 at 40,680,365 bp
  • C to A, chromosome 8 at 69,102,759 bp
  • C to T, chromosome 8 at 94,304,191 bp
  • T to C, chromosome 9 at 27,333,496 bp
  • T to A, chromosome 9 at 44,243,017 bp
  • A to G, chromosome 9 at 59,894,180 bp
  • G to T, chromosome 9 at 62,796,738 bp
  • G to T, chromosome 9 at 87,244,331 bp
  • A to G, chromosome 10 at 14,128,363 bp
  • T to A, chromosome 10 at 20,339,878 bp
  • G to A, chromosome 10 at 80,865,617 bp
  • T to C, chromosome 10 at 100,568,914 bp
  • C to A, chromosome 11 at 32,821,870 bp
  • C to T, chromosome 11 at 50,397,784 bp
  • A to G, chromosome 12 at 8,974,955 bp
  • A to G, chromosome 12 at 101,989,829 bp
  • C to T, chromosome 13 at 56,084,262 bp
  • G to A, chromosome 13 at 70,779,518 bp
  • A to G, chromosome 14 at 12,465,935 bp
  • T to C, chromosome 14 at 103,577,820 bp
  • A to C, chromosome 15 at 55,064,646 bp
  • C to A, chromosome 15 at 64,822,207 bp
  • A to T, chromosome 15 at 66,674,011 bp
  • C to T, chromosome 15 at 79,129,836 bp
  • G to A, chromosome 15 at 85,844,503 bp
  • T to C, chromosome 15 at 103,455,934 bp
  • T to C, chromosome 17 at 30,731,000 bp
  • T to C, chromosome 17 at 85,985,223 bp
  • C to T, chromosome 19 at 3,981,133 bp
  • T to A, chromosome 19 at 7,095,358 bp
  • G to T, chromosome 19 at 34,619,230 bp
  • C to A, chromosome 19 at 36,733,698 bp
  • C to T, chromosome 19 at 60,851,020 bp
  • T to C, chromosome X at 20,851,673 bp
  • A to G, chromosome X at 100,593,947 bp
  • T to G, chromosome X at 143,804,789 bp
  • G to C, chromosome X at 147,194,190 bp
  • G to T, chromosome X at 147,194,192 bp
  • A to T, chromosome X at 150,655,481 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2228 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040229-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.