Strain Name:
C57BL/6J-MtgxR2237Btlr/Mmmh
Stock Number:
040237-MU
Citation ID:
RRID:MMRRC_040237-MU
Other Names:
R2237 (G1), C57BL/6J-MtgxR2237Btlr
Major Collection:

Gene Information

Gene Symbol: Espl1 [MGI:2146156] (Mus musculus (mouse))
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, PRCE, Cerp, separase, ESP1, SSE
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Eomes [MGI:1201683] (Mus musculus (mouse))
Name: eomesodermin
Synonyms: Tbr2
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Pik3cb [MGI:1922019] (Mus musculus (mouse))
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Rbpms2 [MGI:1919223] (Mus musculus (mouse))
Name: RNA binding protein with multiple splicing 2
Synonyms: 2400008B06Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Skap2 [MGI:1889206] (Mus musculus (mouse))
Name: src family associated phosphoprotein 2
Synonyms: 2610021A10Rik, RA70, mSKAP55R, SKAP-HOM, Saps
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: L3mbtl2 [MGI:2443584] (Mus musculus (mouse))
Name: L3MBTL2 polycomb repressive complex 1 subunit
Synonyms: m4mbt, 4732493N06Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Brd7 [MGI:1349766] (Mus musculus (mouse))
Name: bromodomain containing 7
Synonyms: CELTIX1, bromodomain protein 75 kDa, BP75
Chromosome: 8
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 26,685,160 bp
  • G to A, chromosome 1 at 71,074,976 bp
  • G to A, chromosome 1 at 87,796,690 bp
  • A to T, chromosome 1 at 136,345,331 bp
  • A to G, chromosome 2 at 49,694,008 bp
  • T to A, chromosome 2 at 62,268,646 bp
  • G to A, chromosome 2 at 75,676,554 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • T to A, chromosome 4 at 109,156,657 bp
  • A to G, chromosome 4 at 118,709,995 bp
  • A to C, chromosome 4 at 134,202,205 bp
  • A to G, chromosome 4 at 139,442,790 bp
  • A to T, chromosome 4 at 155,862,218 bp
  • T to C, chromosome 5 at 3,618,915 bp
  • T to A, chromosome 5 at 8,408,542 bp
  • C to T, chromosome 5 at 14,713,938 bp
  • A to T, chromosome 5 at 24,548,294 bp
  • T to C, chromosome 5 at 37,378,183 bp
  • T to A, chromosome 5 at 69,110,409 bp
  • T to A, chromosome 5 at 75,181,508 bp
  • T to C, chromosome 5 at 76,559,710 bp
  • T to C, chromosome 5 at 123,803,670 bp
  • A to G, chromosome 5 at 136,408,041 bp
  • G to A, chromosome 5 at 137,457,837 bp
  • G to A, chromosome 5 at 142,474,865 bp
  • C to A, chromosome 6 at 51,908,025 bp
  • T to C, chromosome 6 at 55,301,070 bp
  • C to T, chromosome 6 at 85,932,904 bp
  • T to C, chromosome 6 at 91,498,108 bp
  • T to C, chromosome 6 at 118,652,743 bp
  • A to G, chromosome 7 at 5,097,602 bp
  • G to A, chromosome 7 at 17,873,621 bp
  • T to C, chromosome 7 at 43,354,985 bp
  • T to C, chromosome 7 at 118,490,071 bp
  • T to G, chromosome 7 at 141,862,089 bp
  • T to C, chromosome 8 at 24,646,287 bp
  • G to A, chromosome 8 at 48,342,337 bp
  • A to C, chromosome 8 at 54,602,629 bp
  • T to C, chromosome 8 at 84,633,765 bp
  • T to A, chromosome 8 at 88,346,913 bp
  • C to T, chromosome 8 at 95,040,544 bp
  • T to C, chromosome 9 at 14,308,465 bp
  • A to C, chromosome 9 at 15,575,301 bp
  • G to A, chromosome 9 at 21,142,533 bp
  • T to C, chromosome 9 at 44,710,920 bp
  • C to A, chromosome 9 at 65,651,611 bp
  • T to C, chromosome 9 at 92,290,824 bp
  • A to G, chromosome 9 at 99,041,028 bp
  • A to G, chromosome 9 at 109,555,235 bp
  • G to A, chromosome 9 at 111,690,122 bp
  • A to G, chromosome 9 at 118,482,291 bp
  • T to C, chromosome 9 at 119,138,191 bp
  • C to T, chromosome 9 at 122,771,147 bp
  • G to A, chromosome 10 at 79,168,270 bp
  • T to C, chromosome 10 at 80,702,830 bp
  • A to G, chromosome 11 at 4,238,505 bp
  • T to G, chromosome 11 at 29,569,329 bp
  • A to G, chromosome 11 at 59,852,870 bp
  • A to C, chromosome 11 at 69,185,771 bp
  • C to T, chromosome 11 at 96,955,936 bp
  • G to A, chromosome 11 at 101,079,826 bp
  • C to A, chromosome 12 at 10,375,558 bp
  • T to A, chromosome 12 at 75,007,719 bp
  • T to C, chromosome 12 at 91,347,567 bp
  • G to A, chromosome 12 at 114,724,147 bp
  • A to G, chromosome 12 at 117,874,026 bp
  • T to A, chromosome 13 at 11,662,260 bp
  • T to C, chromosome 13 at 13,500,485 bp
  • G to A, chromosome 13 at 20,794,311 bp
  • T to G, chromosome 14 at 70,171,249 bp
  • A to T, chromosome 14 at 72,574,339 bp
  • A to T, chromosome 14 at 78,299,981 bp
  • A to T, chromosome 15 at 81,684,330 bp
  • G to A, chromosome 15 at 102,315,569 bp
  • T to C, chromosome 16 at 14,443,339 bp
  • T to G, chromosome 16 at 30,338,422 bp
  • T to A, chromosome 16 at 44,820,897 bp
  • G to A, chromosome 17 at 7,352,123 bp
  • T to C, chromosome 17 at 30,334,328 bp
  • C to A, chromosome 17 at 75,310,163 bp
  • T to A, chromosome 18 at 61,504,406 bp
  • T to A, chromosome 19 at 6,337,590 bp
  • T to C, chromosome 19 at 11,828,450 bp
  • T to C, chromosome 19 at 40,845,775 bp
  • G to T, chromosome X at 49,493,405 bp
  • G to T, chromosome X at 57,066,504 bp
  • A to G, chromosome X at 153,833,676 bp
  • A to G, chromosome X at 160,541,412 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2237 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040237-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.