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Strain Name:
C57BL/6J-MtgxR2240Btlr/Mmmh
Stock Number:
040240-MU
Citation ID:
RRID:MMRRC_040240-MU
Other Names:
R2240 (G1), C57BL/6J-MtgxR2240Btlr
Major Collection:

Strain Information

Mafb
Name: MAF bZIP transcription factor B
Synonyms: Kreisler, Krml1, Krml
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16658
HGNC: HGNC:6408
Homologene: 31315
Foxp4
Name: forkhead box P4
Synonyms: 1200010K03Rik, 2310007G05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74123
Homologene: 12536
Neurog1
Name: neurogenin 1
Synonyms: neurogenin, Math4C, ngn1, neurogenin 1, Neurod3, bHLHa6
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18014
VEGA: 13
HGNC: HGNC:7764
Homologene: 4490
Dennd2b
Name: DENN domain containing 2B
Synonyms: 2610305K15Rik, 2010004M01Rik, St5, Denn2b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76954
Homologene: 3951
Cbx7
Name: chromobox 7
Synonyms: 1600014J01Rik, D15Ertd417e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 52609
HGNC: HGNC:1557
Homologene: 65296
Brd4
Name: bromodomain containing 4
Synonyms: MCAP, HUNK1, WI-11513
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 57261
Homologene: 136213
Gtf2a1
Name: general transcription factor II A, 1
Synonyms: TfIIAa/b, 19kDa, 37kDa, 6330549H03Rik, Tfiia1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 83602
HGNC: HGNC:4646
Homologene: 56331
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 24,179,501 bp
  • A to G, chromosome 1 at 33,739,325 bp
  • T to C, chromosome 1 at 40,105,470 bp
  • T to C, chromosome 1 at 52,660,819 bp
  • A to G, chromosome 1 at 58,452,129 bp
  • A to T, chromosome 1 at 164,280,027 bp
  • A to G, chromosome 1 at 178,715,923 bp
  • A to G, chromosome 2 at 31,800,505 bp
  • G to A, chromosome 2 at 52,050,914 bp
  • G to A, chromosome 2 at 69,696,953 bp
  • A to G, chromosome 2 at 74,869,352 bp
  • G to A, chromosome 2 at 103,274,075 bp
  • C to T, chromosome 2 at 104,156,816 bp
  • C to T, chromosome 2 at 125,740,737 bp
  • A to T, chromosome 2 at 160,366,027 bp
  • C to T, chromosome 3 at 31,111,622 bp
  • C to A, chromosome 3 at 64,259,062 bp
  • T to A, chromosome 3 at 68,694,184 bp
  • T to C, chromosome 3 at 95,240,932 bp
  • T to G, chromosome 3 at 96,920,302 bp
  • C to T, chromosome 3 at 97,724,164 bp
  • T to A, chromosome 3 at 122,011,453 bp
  • T to C, chromosome 3 at 138,427,956 bp
  • T to C, chromosome 3 at 144,825,935 bp
  • T to A, chromosome 3 at 145,008,985 bp
  • C to A, chromosome 4 at 32,765,701 bp
  • G to T, chromosome 4 at 59,886,515 bp
  • C to A, chromosome 4 at 144,272,936 bp
  • A to T, chromosome 4 at 145,110,895 bp
  • C to T, chromosome 4 at 145,585,321 bp
  • T to C, chromosome 4 at 151,084,575 bp
  • A to G, chromosome 5 at 92,431,027 bp
  • A to G, chromosome 5 at 108,444,707 bp
  • C to CAT, chromosome 6 at 116,042,870 bp
  • T to C, chromosome 6 at 128,675,882 bp
  • A to T, chromosome 7 at 5,884,370 bp
  • G to A, chromosome 7 at 19,286,829 bp
  • A to G, chromosome 7 at 46,241,029 bp
  • A to T, chromosome 7 at 48,643,641 bp
  • T to C, chromosome 7 at 109,538,923 bp
  • C to A, chromosome 7 at 127,905,418 bp
  • G to T, chromosome 7 at 129,605,694 bp
  • A to C, chromosome 8 at 104,962,502 bp
  • C to A, chromosome 8 at 124,030,139 bp
  • A to G, chromosome 9 at 20,027,144 bp
  • A to T, chromosome 9 at 20,437,267 bp
  • A to T, chromosome 9 at 89,976,762 bp
  • A to G, chromosome 10 at 70,946,803 bp
  • T to C, chromosome 11 at 5,803,265 bp
  • A to T, chromosome 11 at 46,678,216 bp
  • A to G, chromosome 11 at 97,351,733 bp
  • T to C, chromosome 12 at 31,457,072 bp
  • G to A, chromosome 12 at 59,107,882 bp
  • A to T, chromosome 12 at 91,586,739 bp
  • A to G, chromosome 12 at 104,412,487 bp
  • T to A, chromosome 13 at 8,861,232 bp
  • G to A, chromosome 13 at 24,982,507 bp
  • T to C, chromosome 13 at 54,599,154 bp
  • T to C, chromosome 13 at 56,251,535 bp
  • T to A, chromosome 13 at 114,828,467 bp
  • G to A, chromosome 14 at 19,805,914 bp
  • T to C, chromosome 14 at 20,765,446 bp
  • A to G, chromosome 14 at 29,989,757 bp
  • T to C, chromosome 14 at 31,299,974 bp
  • A to C, chromosome 14 at 32,835,868 bp
  • A to T, chromosome 14 at 55,899,667 bp
  • T to A, chromosome 14 at 118,995,049 bp
  • A to G, chromosome 15 at 34,433,063 bp
  • T to C, chromosome 15 at 76,206,050 bp
  • C to A, chromosome 15 at 79,918,357 bp
  • C to T, chromosome 15 at 101,054,035 bp
  • G to A, chromosome 17 at 12,975,465 bp
  • T to A, chromosome 17 at 23,996,781 bp
  • T to A, chromosome 17 at 24,376,443 bp
  • T to A, chromosome 17 at 28,778,111 bp
  • T to C, chromosome 17 at 32,213,639 bp
  • T to C, chromosome 17 at 35,982,679 bp
  • C to A, chromosome 17 at 47,871,276 bp
  • C to T, chromosome 18 at 11,677,669 bp
  • A to C, chromosome 19 at 12,463,038 bp
  • G to T, chromosome 19 at 17,329,331 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2240 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040240-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.