Strain Name:
C57BL/6J-MtgxR2241Btlr/Mmmh
Stock Number:
040241-MU
Citation ID:
RRID:MMRRC_040241-MU
Other Names:
R2241 (G1), C57BL/6J-MtgxR2241Btlr
Major Collection:

Gene Information

Nae1
Name: NEDD8 activating enzyme E1 subunit 1
Synonyms: Appbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234664
HGNC: HGNC:621
Homologene: 68370
Wasf1
Name: WAS protein family, member 1
Synonyms: Scar, WAVE, WAVE-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 83767
Homologene: 2920
Fzd6
Name: frizzled class receptor 6
Synonyms: Fz6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 14368
VEGA: 15
HGNC: HGNC:4044
Homologene: 2617
Rsf1
Name: remodeling and spacing factor 1
Synonyms: XAP8, p325, 4832420A03Rik, C030033M12Rik, Hbxap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233532
Homologene: 41142
Cep350
Name: centrosomal protein 350
Synonyms: 4933409L06Rik, 6430546F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74081
Homologene: 8879
Tep1
Name: telomerase associated protein 1
Synonyms: Tp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 21745
VEGA: 14
Homologene: 5157
Aftph
Name: aftiphilin
Synonyms: 9130023F12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216549
Homologene: 9764
Prune2
Name: prune homolog 2
Synonyms: 6330414G02Rik, A230083H22Rik, A330102H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 353211
VEGA: 19
Homologene: 18939
Clca4b
Name: chloride channel accessory 4B
Synonyms: AI747448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99709
HGNC: HGNC:2018
Homologene: 40808
Yme1l1
Name: YME1-like 1 (S. cerevisiae)
Synonyms: ATP-dependent metalloprotease FtsH1, Ftsh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 27377
Homologene: 31996
Ddx20
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Synonyms: GEMIN3, dp103
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 53975
HGNC: HGNC:2743
Homologene: 5214
Cyb5b
Name: cytochrome b5 type B
Synonyms: 1810044O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 66427
Homologene: 23546
Lrch3
Name: leucine-rich repeats and calponin homology (CH) domain containing 3
Synonyms: 2210409B11Rik, LOC385628
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 70144
Homologene: 13111
Sec24b
Name: Sec24 related gene family, member B (S. cerevisiae)
Synonyms: SEC24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99683
Homologene: 55968
Kcnj14
Name: potassium inwardly-rectifying channel, subfamily J, member 14
Synonyms: A930026G01Rik, Kir2.4, IRK4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 211480
HGNC: HGNC:6260
Homologene: 27086
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328783
VEGA: 17
Homologene: 18633
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Itga4
Name: integrin alpha 4
Synonyms: VLA-4 receptor, alpha 4 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16401
HGNC: HGNC:6140
Homologene: 37364
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Gm20114, Shn-2, Schnurri-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Pcnx4
Name: pecanex homolog 4
Synonyms: Pcnxl4, 1810048J11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 67708
VEGA: 12
Homologene: 23366
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 545030
Homologene: 83473
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20192
Homologene: 68151
Adcy8
Name: adenylate cyclase 8
Synonyms: AC8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 11514
VEGA: 15
HGNC: HGNC:239
Homologene: 37443
Farp2
Name: FERM, RhoGEF and pleckstrin domain protein 2
Synonyms: Fir, D030026M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227377
Homologene: 8877
Vmn2r102
Name: vomeronasal 2, receptor 102
Synonyms: EG224572
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224572
Homologene: 115024
Unc13a
Name: unc-13 homolog A
Synonyms: Munc13-1, 2410078G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 382018
Homologene: 11279
Olfr467
Name: olfactory receptor 467
Synonyms: MOR204-33P, GA_x6K02T2PBJ9-10144091-10145011
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 257919
Homologene: 81542
Olfr801
Name: olfactory receptor 801
Synonyms: GA_x6K02T2PULF-11349138-11348182, MOR110-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 258282
Homologene: 138306
Serpina3m
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3M
Synonyms: Spi2.4, contrapsin-like, MMSPi2.4, Spi2-rs1, antitrypsin, alpha-1 antiproteinase, Spi-2l, 3e46, MMCM7, Spi-2rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 20717
HGNC: HGNC:16
Homologene: 111129
Dapk2
Name: death-associated protein kinase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 13143
HGNC: HGNC:2675
Homologene: 74940
Gpr149
Name: G protein-coupled receptor 149
Synonyms: PGR10, Ieda, R35, 9630018L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229357
Homologene: 16359
Mterf2
Name: mitochondrial transcription termination factor 2
Synonyms: Mterfd3, 1700007D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 74238
VEGA: 10
Homologene: 11869
Olfr912
Name: olfactory receptor 912
Synonyms: MOR165-4, GA_x6K02T2PVTD-32283590-32284522
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258806
VEGA: 9
Homologene: 115510
Polh
Name: polymerase (DNA directed), eta (RAD 30 related)
Synonyms: RAD30A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 80905
VEGA: 17
HGNC: HGNC:9181
Homologene: 38189
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 93,579,903 bp
  • C to T, chromosome 1 at 155,958,556 bp
  • C to T, chromosome 2 at 23,196,900 bp
  • T to A, chromosome 2 at 79,301,013 bp
  • A to G, chromosome 2 at 112,801,392 bp
  • A to G, chromosome 3 at 62,604,053 bp
  • A to C, chromosome 3 at 105,683,205 bp
  • A to G, chromosome 3 at 130,030,518 bp
  • A to T, chromosome 3 at 144,911,226 bp
  • C to T, chromosome 7 at 45,819,901 bp
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp
  • T to C, chromosome 7 at 107,814,833 bp
  • G to A, chromosome 8 at 71,655,657 bp
  • A to C, chromosome 8 at 104,519,788 bp
  • A to G, chromosome 8 at 107,170,413 bp
  • A to G, chromosome 9 at 38,581,805 bp
  • C to A, chromosome 9 at 66,251,072 bp
  • A to G, chromosome 9 at 79,603,448 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • G to T, chromosome 10 at 40,936,750 bp
  • T to C, chromosome 10 at 85,120,316 bp
  • G to A, chromosome 10 at 129,669,895 bp
  • T to C, chromosome 11 at 20,726,328 bp
  • T to C, chromosome 12 at 72,574,154 bp
  • G to A, chromosome 12 at 104,389,449 bp
  • A to T, chromosome 14 at 33,073,511 bp
  • G to C, chromosome 14 at 50,854,210 bp
  • G to T, chromosome 15 at 39,031,536 bp
  • G to T, chromosome 15 at 64,699,381 bp
  • A to G, chromosome 16 at 32,995,841 bp
  • T to C, chromosome 17 at 19,676,741 bp
  • G to A, chromosome 17 at 25,742,934 bp
  • A to T, chromosome 17 at 46,188,136 bp
  • T to A, chromosome 19 at 17,123,092 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2241 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040241-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.