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Strain Name:
C57BL/6J-MtgxR2251Btlr/Mmmh
Stock Number:
040251-MU
Citation ID:
RRID:MMRRC_040251-MU
Other Names:
R2251 (G1), C57BL/6J-MtgxR2251Btlr
Major Collection:

Strain Information

Fgb
Name: fibrinogen beta chain
Synonyms: 2510049G14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 110135
HGNC: HGNC:3662
Homologene: 3772
Etv5
Name: ets variant 5
Synonyms: 8430401F14Rik, erm, 1110005E01Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 104156
HGNC: HGNC:3494
Homologene: 3276
Gch1
Name: GTP cyclohydrolase 1
Synonyms: GTPCH, GTP-CH
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14528
VEGA: 14
HGNC: HGNC:4193
Homologene: 132
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: med, seal, motor end-plate disease, nmf2, ataxia 3, mnd2, mnd-2, C630029C19Rik, nmf58, NaCh6, Nav1.6, nmf335, NMF335, nur14
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20273
Homologene: 7927
Slc6a4
Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Synonyms: 5-HTT, Htt, Sert
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15567
Homologene: 817
Tm9sf2
Name: transmembrane 9 superfamily member 2
Synonyms: P76, 1500001N15Rik, D14Ertd64e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68059
Homologene: 21004
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 132,525,321 bp
  • A to T, chromosome 1 at 187,048,473 bp
  • T to A, chromosome 2 at 31,779,119 bp
  • A to G, chromosome 2 at 85,353,858 bp
  • A to G, chromosome 2 at 111,306,310 bp
  • T to C, chromosome 2 at 118,723,765 bp
  • T to C, chromosome 2 at 148,683,936 bp
  • T to G, chromosome 3 at 83,043,284 bp
  • A to T, chromosome 3 at 96,057,040 bp
  • A to T, chromosome 3 at 129,284,196 bp
  • T to A, chromosome 4 at 83,635,626 bp
  • T to A, chromosome 4 at 108,520,208 bp
  • A to G, chromosome 4 at 129,725,730 bp
  • A to C, chromosome 4 at 155,250,553 bp
  • G to A, chromosome 5 at 65,806,728 bp
  • T to C, chromosome 6 at 23,649,175 bp
  • A to T, chromosome 7 at 24,585,590 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • A to G, chromosome 7 at 49,453,277 bp
  • G to A, chromosome 7 at 64,209,976 bp
  • C to T, chromosome 7 at 75,739,477 bp
  • A to T, chromosome 7 at 104,996,595 bp
  • T to C, chromosome 7 at 127,137,159 bp
  • T to C, chromosome 7 at 127,344,378 bp
  • A to G, chromosome 8 at 33,635,364 bp
  • T to C, chromosome 8 at 40,905,621 bp
  • G to T, chromosome 8 at 46,637,955 bp
  • A to G, chromosome 8 at 48,292,277 bp
  • A to T, chromosome 8 at 71,692,818 bp
  • T to C, chromosome 8 at 84,716,170 bp
  • A to G, chromosome 8 at 95,763,186 bp
  • T to C, chromosome 8 at 117,057,438 bp
  • T to C, chromosome 9 at 66,562,578 bp
  • T to A, chromosome 9 at 99,502,600 bp
  • T to A, chromosome 9 at 108,971,961 bp
  • G to T, chromosome 10 at 63,024,605 bp
  • T to A, chromosome 10 at 78,181,088 bp
  • T to C, chromosome 10 at 84,605,069 bp
  • C to A, chromosome 11 at 16,368,273 bp
  • A to G, chromosome 11 at 54,561,406 bp
  • T to A, chromosome 11 at 59,465,493 bp
  • T to C, chromosome 11 at 77,016,616 bp
  • T to C, chromosome 13 at 22,102,548 bp
  • T to C, chromosome 14 at 47,189,341 bp
  • T to A, chromosome 14 at 50,360,792 bp
  • T to C, chromosome 14 at 55,023,395 bp
  • G to A, chromosome 14 at 59,142,612 bp
  • C to T, chromosome 14 at 64,742,298 bp
  • T to A, chromosome 14 at 122,139,731 bp
  • A to G, chromosome 15 at 74,762,593 bp
  • T to C, chromosome 15 at 99,945,097 bp
  • A to G, chromosome 15 at 101,017,106 bp
  • T to C, chromosome 16 at 18,875,375 bp
  • T to C, chromosome 16 at 22,421,878 bp
  • A to T, chromosome 16 at 22,622,260 bp
  • T to C, chromosome 16 at 36,021,569 bp
  • T to C, chromosome 16 at 92,310,828 bp
  • G to A, chromosome 17 at 7,753,607 bp
  • A to G, chromosome 17 at 34,211,954 bp
  • A to C, chromosome 17 at 36,325,606 bp
  • C to T, chromosome 17 at 38,274,903 bp
  • C to T, chromosome 17 at 48,366,057 bp
  • G to T, chromosome 18 at 39,486,751 bp
  • A to T, chromosome X at 157,559,033 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2251 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040251-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.