Strain Name:
C57BL/6J-MtgxR2254Btlr/Mmmh
Stock Number:
040254-MU
Citation ID:
RRID:MMRRC_040254-MU
Other Names:
R2254 (G1), C57BL/6J-MtgxR2254Btlr
Major Collection:

Gene Information

Kitl
Name: kit ligand
Synonyms: Gb, SCF, Steel, Mgf, Sl, Kitlg, SF, grizzle-belly, blz, stem cell factor, Steel factor, SLF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Gabrg1
Name: gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Oxtr
Name: oxytocin receptor
Synonyms: OTR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 18430
HGNC: HGNC:8529
Homologene: 20255
Gdi2
Name: guanosine diphosphate (GDP) dissociation inhibitor 2
Synonyms: GDI beta, GDIB, Gdi3, GDI-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 14569
VEGA: 13
HGNC: HGNC:4227
Homologene: 37488
Prdm5
Name: PR domain containing 5
Synonyms: 6530401I24Rik, 4432417F03Rik, PFM2, E130112L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 70779
HGNC: HGNC:9349
Homologene: 10274
Nup93
Name: nucleoporin 93
Synonyms: 2410008G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 71805
Homologene: 40971
Gpi1
Name: glucose phosphate isomerase 1
Synonyms: maturation factor, NK, Org, Gpi-1t, autocrine motility factor, NK/GPI, neuroleukin, Gpi1-r, Gpi-1, MF, Gpi-1r, Gpi1-s, Gpi-1s, Gpi1-t, AMF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 14751
HGNC: HGNC:4458
Homologene: 145
Zfp64
Name: zinc finger protein 64
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22722
Homologene: 7604
Trim24
Name: tripartite motif-containing 24
Synonyms: Tif1a, D430004I05Rik, A130082H20Rik, TIF1alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 21848
Homologene: 20830
Chmp7
Name: charged multivesicular body protein 7
Synonyms: 6330407G04Rik, CHMP family, member 7, 4930596K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105513
VEGA: 14
Homologene: 14613
Glyr1
Name: glyoxylate reductase 1 homolog (Arabidopsis)
Synonyms: 3930401K13Rik, Npac, 2810419J22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 74022
VEGA: 16
Homologene: 12525
Golt1b
Name: golgi transport 1B
Synonyms: 2310061A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 66964
Homologene: 41102
Wdr62
Name: WD repeat domain 62
Synonyms: b2b1508Clo, 2310038K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233064
Homologene: 15927
Scube2
Name: signal peptide, CUB domain, EGF-like 2
Synonyms: ICRFP703B1614Q5.1, Cegf1, ICRFP703N2430Q5.1, 4932442O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 56788
Homologene: 36383
Mtmr2
Name: myotubularin related protein 2
Synonyms: 6030445P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 77116
HGNC: HGNC:7450
Homologene: 22951
Lepr
Name: leptin receptor
Synonyms: Leprb, LEPROT, Modb1, OB-RGRP, leptin receptor gene-related protein, obl, obese-like, Obr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 16847
HGNC: HGNC:6554
Homologene: 1731
Krt33a
Name: keratin 33A
Synonyms: 2310015J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 71888
Homologene: 74433
Apob
Name: apolipoprotein B
Synonyms: apob-48, apob-100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Rbm39
Name: RNA binding motif protein 39
Synonyms: Caper alpha, 2310040E03Rik, B330012G18Rik, 1500012C14Rik, Rnpc2, caper
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 170791
Homologene: 136465
Prap1
Name: proline-rich acidic protein 1
Synonyms: Upa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22264
Homologene: 130637
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77505
Homologene: 131117
Bmper
Name: BMP-binding endothelial regulator
Synonyms: CV-2, Crim3, Cv2, 3110056H04Rik, crossveinless-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 73230
VEGA: 9
Homologene: 12494
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
5430419D17Rik
Name: RIKEN cDNA 5430419D17 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 71395
HGNC: null
Homologene: 138430
Slc4a1
Name: solute carrier family 4 (anion exchanger), member 1
Synonyms: l11Jus51, Ae1, erythrocyte membrane protein band 3, band 3, Empb3, CD233
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20533
Homologene: 133556
Tbx15
Name: T-box 15
Synonyms: Tbx14, de, Tbx8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 21384
Homologene: 7967
Arhgef25
Name: Rho guanine nucleotide exchange factor (GEF) 25
Synonyms: GEFT, 2410008H17Rik, D10Ertd610e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 52666
VEGA: 10
Homologene: 32691
Actn2
Name: actinin alpha 2
Synonyms: 1110008F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 11472
HGNC: HGNC:164
Homologene: 31016
Capn3
Name: calpain 3
Synonyms: Capa3, Lp82, p94, Capa-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12335
HGNC: HGNC:1480
Homologene: 52
Steap1
Name: six transmembrane epithelial antigen of the prostate 1
Synonyms: 2410007B19Rik, Prss24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 70358
Homologene: 8256
Map1a
Name: microtubule-associated protein 1 A
Synonyms: Mtap1, 6330416M19Rik, Mtap-1, Mtap1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17754
HGNC: HGNC:6835
Homologene: 1778
Tas2r120
Name: taste receptor, type 2, member 120
Synonyms: mt2r47, T2R20, Tas2r20, mGR20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 387348
Homologene: 135705
Cdh2
Name: cadherin 2
Synonyms: Ncad, N-CAD, N-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 12558
HGNC: HGNC:1759
Homologene: 20424
Ano9
Name: anoctamin 9
Synonyms: Tmem16j, 5430425C04Rik, Tp53i5, Trp53i5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 71345
Homologene: 67083
Engase
Name: endo-beta-N-acetylglucosaminidase
Synonyms: D230014K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217364
Homologene: 13666
Atp8a1
Name: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Synonyms: Atp3a2, B230107D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11980
Homologene: 48402
Kcnh1
Name: potassium voltage-gated channel, subfamily H (eag-related), member 1
Synonyms: ether a go-go, Kv10.1, Eag1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16510
HGNC: HGNC:6250
Homologene: 68242
Ovgp1
Name: oviductal glycoprotein 1
Synonyms: OGP, Chit5, MOGP, muc9, mucin 9, oviductin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12659
HGNC: HGNC:8524
Homologene: 74442
Scg2
Name: secretogranin II
Synonyms: SgII, Chgc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20254
Homologene: 2591
Lrrcc1
Name: leucine rich repeat and coiled-coil domain containing 1
Synonyms: 4932441F23Rik, 1200008A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 71710
Homologene: 12559
Zer1
Name: zyg-11 related, cell cycle regulator
Synonyms: Zyg11bl, C230075L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227693
Homologene: 4621
Lax1
Name: lymphocyte transmembrane adaptor 1
Synonyms: E430019B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240754
Homologene: 49504
Ovch2
Name: ovochymase 2
Synonyms: 9230106D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 244199
Homologene: 18255
Upb1
Name: ureidopropionase, beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 103149
Homologene: 9471
Ube2z
Name: ubiquitin-conjugating enzyme E2Z
Synonyms: C030047H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 268470
Homologene: 11319
Wdr20rt
Name: WD repeat domain 20, retrogene
Synonyms: Wdr20b, 4930427E19Rik, 4921538B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 70948
VEGA: 12
HGNC: null
Homologene: null
Il18r1
Name: interleukin 18 receptor 1
Synonyms: Il1rrp, Il18ralpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16182
HGNC: HGNC:5988
Homologene: 2861
Ang5
Name: angiogenin, ribonuclease A family, member 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 503844
HGNC: null
Homologene: 134083
Med11
Name: mediator complex subunit 11
Synonyms: 1110030J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 66172
Homologene: 32630
F8
Name: coagulation factor VIII
Synonyms: Cf8, Factor VIII, Cf-8, FVIII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 14069
HGNC: HGNC:3546
Homologene: 49153
Olfr610
Name: olfactory receptor 610
Synonyms: MOR9-2, GA_x6K02T2PBJ9-6221839-6220892
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 259085
HGNC: null
Homologene: 17495
Slc22a28
Name: solute carrier family 22, member 28
Synonyms: Gm5631
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 434674
VEGA: 19
Homologene: 77136
Ifi204
Name: interferon activated gene 204
Synonyms: p204
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 15951
HGNC: HGNC:5395
Homologene: 115929
AI429214
Name: expressed sequence AI429214
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 621080
Homologene: 82747
Zfp40
Name: zinc finger protein 40
Synonyms: Zfp-40, NTfin12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 22700
HGNC: null
Homologene: 136312
Slc22a12
Name: solute carrier family 22 (organic anion/cation transporter), member 12
Synonyms: URAT1, Rst
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 20521
Homologene: 56442
Ccdc90b
Name: coiled-coil domain containing 90B
Synonyms: 2310015N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 66365
Homologene: 23328
B3gnt4
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4
Synonyms: 1010001G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231727
Homologene: 81939
Gzmb
Name: granzyme B
Synonyms: CCP-1/C11, Ctla-1, GZB, Ctla1, CCP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 14939
VEGA: 14
HGNC: null
Homologene: null
Gm5938
Name: predicted gene 5938
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 546335
HGNC: null
Homologene: 79513
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 40,491,220 bp
  • G to A, chromosome 1 at 79,436,500 bp
  • A to G, chromosome 1 at 133,680,233 bp
  • G to A, chromosome 1 at 173,761,730 bp
  • G to T, chromosome 1 at 192,505,414 bp
  • G to A, chromosome 2 at 30,108,274 bp
  • T to A, chromosome 2 at 76,768,340 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • A to G, chromosome 2 at 120,501,251 bp
  • A to G, chromosome 2 at 121,303,791 bp
  • A to G, chromosome 2 at 156,174,089 bp
  • G to A, chromosome 2 at 168,926,742 bp
  • G to A, chromosome 3 at 14,547,255 bp
  • A to G, chromosome 3 at 99,351,874 bp
  • A to G, chromosome 3 at 105,986,912 bp
  • T to C, chromosome 4 at 101,815,112 bp
  • A to C, chromosome 5 at 5,742,816 bp
  • A to T, chromosome 5 at 67,648,588 bp
  • T to A, chromosome 5 at 70,782,364 bp
  • A to C, chromosome 5 at 123,511,279 bp
  • A to G, chromosome 6 at 37,958,677 bp
  • T to C, chromosome 6 at 65,857,631 bp
  • A to G, chromosome 6 at 85,619,848 bp
  • A to T, chromosome 6 at 112,489,106 bp
  • A to T, chromosome 6 at 132,657,609 bp
  • T to C, chromosome 6 at 142,396,253 bp
  • T to G, chromosome 7 at 30,267,903 bp
  • T to C, chromosome 7 at 34,202,877 bp
  • T to A, chromosome 7 at 92,572,568 bp
  • A to G, chromosome 7 at 103,506,064 bp
  • T to A, chromosome 7 at 105,703,772 bp
  • A to G, chromosome 7 at 107,790,195 bp
  • A to G, chromosome 7 at 109,825,459 bp
  • G to T, chromosome 7 at 131,222,905 bp
  • A to G, chromosome 7 at 140,096,162 bp
  • T to A, chromosome 7 at 141,103,090 bp
  • G to T, chromosome 8 at 36,993,766 bp
  • T to C, chromosome 8 at 94,227,857 bp
  • T to C, chromosome 9 at 13,796,057 bp
  • T to A, chromosome 9 at 23,381,463 bp
  • G to A, chromosome 10 at 75,436,217 bp
  • G to A, chromosome 10 at 100,080,131 bp
  • T to C, chromosome 10 at 127,189,521 bp
  • T to C, chromosome 11 at 70,452,095 bp
  • A to T, chromosome 11 at 96,055,965 bp
  • A to T, chromosome 11 at 100,014,178 bp
  • A to G, chromosome 11 at 102,354,446 bp
  • T to C, chromosome 11 at 118,481,266 bp
  • C to T, chromosome 12 at 8,011,256 bp
  • T to A, chromosome 12 at 65,226,233 bp
  • T to A, chromosome 13 at 3,554,400 bp
  • T to C, chromosome 13 at 12,296,479 bp
  • A to G, chromosome 14 at 43,962,617 bp
  • A to G, chromosome 14 at 56,262,089 bp
  • C to T, chromosome 14 at 69,720,956 bp
  • A to G, chromosome 16 at 5,019,013 bp
  • T to C, chromosome 17 at 23,178,370 bp
  • A to T, chromosome 18 at 16,643,928 bp
  • A to T, chromosome 19 at 6,542,541 bp
  • A to C, chromosome 19 at 8,064,493 bp
  • T to A, chromosome X at 75,296,076 bp
  • T to A, chromosome X at 78,128,555 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2254 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040254-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.