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Strain Name:
C57BL/6J-MtgxR2256Btlr/Mmmh
Stock Number:
040256-MU
Citation ID:
RRID:MMRRC_040256-MU
Other Names:
R2256 (G1), C57BL/6J-MtgxR2256Btlr
Major Collection:

Strain Information

Camkk2
Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
Synonyms: 6330570N16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 207565
HGNC: HGNC:1470
Homologene: 32756
Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: 1110067B02Rik, Sema6D-6, Sema6D-5, Sema6D-4, Sema6D-2, Sema6D-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214968
Homologene: 16195
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Myo1e
Name: myosin IE
Synonyms: 2310020N23Rik, 9130023P14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71602
VEGA: 9
HGNC: HGNC:7599
Homologene: 55864
Atf2
Name: activating transcription factor 2
Synonyms: mXBP, CRE-BP, Creb2, D130078H02Rik, ATF-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11909
HGNC: HGNC:784
Homologene: 31061
Clasrp
Name: CLK4-associating serine/arginine rich protein
Synonyms: SWAP2, Srsf16, Sfrs16
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53609
Homologene: 134306
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 45,321,632 bp
  • A to G, chromosome 1 at 60,236,361 bp
  • A to G, chromosome 2 at 28,561,192 bp
  • A to T, chromosome 2 at 73,845,511 bp
  • A to G, chromosome 2 at 76,584,474 bp
  • A to T, chromosome 2 at 77,132,262 bp
  • T to A, chromosome 2 at 82,962,751 bp
  • T to A, chromosome 2 at 85,608,463 bp
  • T to C, chromosome 2 at 89,788,470 bp
  • T to C, chromosome 2 at 111,489,978 bp
  • A to G, chromosome 2 at 124,664,150 bp
  • T to A, chromosome 2 at 129,810,507 bp
  • A to G, chromosome 2 at 169,886,477 bp
  • T to C, chromosome 3 at 84,446,046 bp
  • T to A, chromosome 3 at 97,718,184 bp
  • T to C, chromosome 3 at 98,328,291 bp
  • T to A, chromosome 3 at 127,561,997 bp
  • A to G, chromosome 3 at 142,781,424 bp
  • A to C, chromosome 4 at 111,431,010 bp
  • A to G, chromosome 4 at 126,441,911 bp
  • G to A, chromosome 4 at 140,759,940 bp
  • T to A, chromosome 5 at 4,888,924 bp
  • T to C, chromosome 5 at 8,958,431 bp
  • C to A, chromosome 5 at 24,446,924 bp
  • G to A, chromosome 5 at 24,447,177 bp
  • C to T, chromosome 5 at 24,552,040 bp
  • A to G, chromosome 5 at 25,822,405 bp
  • T to A, chromosome 5 at 29,437,752 bp
  • T to C, chromosome 5 at 38,453,199 bp
  • T to C, chromosome 5 at 38,671,636 bp
  • A to T, chromosome 5 at 65,417,115 bp
  • T to A, chromosome 5 at 73,072,844 bp
  • A to G, chromosome 5 at 86,016,203 bp
  • C to T, chromosome 5 at 91,594,121 bp
  • A to T, chromosome 5 at 106,635,845 bp
  • C to T, chromosome 5 at 115,348,848 bp
  • T to C, chromosome 5 at 122,746,335 bp
  • C to T, chromosome 5 at 124,283,659 bp
  • C to T, chromosome 5 at 136,161,600 bp
  • A to G, chromosome 5 at 137,031,547 bp
  • T to C, chromosome 6 at 48,906,440 bp
  • A to T, chromosome 6 at 120,492,069 bp
  • A to G, chromosome 6 at 120,492,071 bp
  • T to A, chromosome 6 at 123,329,886 bp
  • A to T, chromosome 6 at 142,091,016 bp
  • T to A, chromosome 7 at 4,771,021 bp
  • G to T, chromosome 7 at 19,562,018 bp
  • A to G, chromosome 7 at 19,586,585 bp
  • G to A, chromosome 7 at 26,173,605 bp
  • A to C, chromosome 7 at 28,614,413 bp
  • A to T, chromosome 7 at 30,467,992 bp
  • A to T, chromosome 7 at 42,012,245 bp
  • T to C, chromosome 7 at 44,533,799 bp
  • A to T, chromosome 7 at 45,728,769 bp
  • A to T, chromosome 7 at 45,728,770 bp
  • A to G, chromosome 7 at 72,185,820 bp
  • C to T, chromosome 7 at 100,159,394 bp
  • A to G, chromosome 7 at 102,000,791 bp
  • A to G, chromosome 7 at 105,035,819 bp
  • A to T, chromosome 7 at 107,955,037 bp
  • T to A, chromosome 7 at 114,253,875 bp
  • T to C, chromosome 7 at 131,090,494 bp
  • A to G, chromosome 7 at 131,443,653 bp
  • A to G, chromosome 7 at 143,709,094 bp
  • T to A, chromosome 8 at 21,963,517 bp
  • T to G, chromosome 8 at 21,998,266 bp
  • A to G, chromosome 8 at 44,950,371 bp
  • A to T, chromosome 8 at 95,075,009 bp
  • A to T, chromosome 8 at 95,328,501 bp
  • C to A, chromosome 8 at 106,874,256 bp
  • C to A, chromosome 8 at 123,245,121 bp
  • T to C, chromosome 9 at 32,247,497 bp
  • G to A, chromosome 9 at 70,378,373 bp
  • C to A, chromosome 9 at 87,206,914 bp
  • A to G, chromosome 10 at 23,810,922 bp
  • A to G, chromosome 10 at 24,101,071 bp
  • T to A, chromosome 10 at 67,225,294 bp
  • T to G, chromosome 11 at 5,524,841 bp
  • T to A, chromosome 11 at 73,340,475 bp
  • T to C, chromosome 11 at 113,830,794 bp
  • T to C, chromosome 12 at 4,737,775 bp
  • C to T, chromosome 12 at 30,236,688 bp
  • A to G, chromosome 13 at 55,647,708 bp
  • A to T, chromosome 13 at 62,607,396 bp
  • T to A, chromosome 13 at 74,739,905 bp
  • G to A, chromosome 13 at 76,827,815 bp
  • T to C, chromosome 13 at 81,506,140 bp
  • A to T, chromosome 13 at 102,735,751 bp
  • A to T, chromosome 13 at 112,876,512 bp
  • T to A, chromosome 14 at 43,962,521 bp
  • A to T, chromosome 14 at 122,941,408 bp
  • A to G, chromosome 16 at 77,113,794 bp
  • A to T, chromosome 17 at 40,956,319 bp
  • AC to A, chromosome 17 at 47,433,423 bp
  • T to A, chromosome 17 at 58,330,315 bp
  • A to G, chromosome 17 at 74,445,630 bp
  • A to T, chromosome 18 at 5,213,508 bp
  • C to A, chromosome 18 at 9,993,466 bp
  • A to G, chromosome 18 at 10,503,307 bp
  • A to G, chromosome X at 140,990,235 bp
  • A to T, chromosome X at 155,027,859 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2256 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040256-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.