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Strain Name:
C57BL/6J-MtgxR2262Btlr/Mmmh
Stock Number:
040262-MU
Citation ID:
RRID:MMRRC_040262-MU
Other Names:
R2262 (G1), C57BL/6J-MtgxR2262Btlr
Major Collection:

Strain Information

Sptbn4
Name: spectrin beta, non-erythrocytic 4
Synonyms: dyn, SpbIV, neuroaxonal dystrophy, 5830426A08Rik, ROSA62, nmf261, 1700022P15Rik, Spnb4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80297
Homologene: 11879
Dnajc3
Name: DnaJ heat shock protein family (Hsp40) member C3
Synonyms: p58IPK, mp58, Prkri, Dnajc3, Dnajc3a, Dnajc3b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100037258
VEGA: 14
HGNC: HGNC:9439
Homologene: 2486
Plekhj1
Name: pleckstrin homology domain containing, family J member 1
Synonyms: 9530063M10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 78670
VEGA: 10
Homologene: 11389
Cdk17
Name: cyclin dependent kinase 17
Synonyms: 6430598J10Rik, Pctk2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237459
VEGA: 10
HGNC: HGNC:8750
Homologene: 55666
Vcp
Name: valosin containing protein
Synonyms: CDC48, p97, p97/VCP, AAA ATPase p97
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269523
Homologene: 5168
Il17rd
Name: interleukin 17 receptor D
Synonyms: Sef, Sef-S, 2810004A10Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 171463
VEGA: 14
Homologene: 9717
Ahdc1
Name: AT hook, DNA binding motif, containing 1
Synonyms: D030015G18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230793
Homologene: 17144
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 2 at 80,128,931 bp
  • T to C, chromosome 2 at 84,345,173 bp
  • G to T, chromosome 2 at 90,757,404 bp
  • C to G, chromosome 2 at 112,675,873 bp
  • T to C, chromosome 2 at 142,740,917 bp
  • T to C, chromosome 2 at 172,427,254 bp
  • T to C, chromosome 3 at 8,541,895 bp
  • T to C, chromosome 3 at 64,556,669 bp
  • A to T, chromosome 3 at 72,929,446 bp
  • T to C, chromosome 4 at 42,980,828 bp
  • C to T, chromosome 4 at 47,017,518 bp
  • A to T, chromosome 4 at 62,191,636 bp
  • A to G, chromosome 4 at 68,829,354 bp
  • C to T, chromosome 4 at 86,478,975 bp
  • A to T, chromosome 4 at 116,052,950 bp
  • A to G, chromosome 4 at 133,063,163 bp
  • G to T, chromosome 4 at 148,929,099 bp
  • T to C, chromosome 5 at 75,185,523 bp
  • G to A, chromosome 5 at 77,309,804 bp
  • T to C, chromosome 5 at 105,016,133 bp
  • T to C, chromosome 5 at 113,942,703 bp
  • A to C, chromosome 5 at 123,878,586 bp
  • A to G, chromosome 5 at 127,657,112 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • A to T, chromosome 6 at 124,506,948 bp
  • A to G, chromosome 6 at 129,874,794 bp
  • T to C, chromosome 6 at 145,138,171 bp
  • T to A, chromosome 7 at 7,240,086 bp
  • T to A, chromosome 7 at 7,474,619 bp
  • T to A, chromosome 7 at 9,217,944 bp
  • T to C, chromosome 7 at 10,527,445 bp
  • T to G, chromosome 7 at 24,104,624 bp
  • T to A, chromosome 7 at 27,434,357 bp
  • T to C, chromosome 7 at 29,532,562 bp
  • T to A, chromosome 7 at 34,676,010 bp
  • G to T, chromosome 7 at 42,928,607 bp
  • C to T, chromosome 7 at 50,560,821 bp
  • C to T, chromosome 7 at 83,793,372 bp
  • T to C, chromosome 7 at 85,136,974 bp
  • A to G, chromosome 8 at 34,873,016 bp
  • A to T, chromosome 8 at 94,866,512 bp
  • T to A, chromosome 8 at 105,016,559 bp
  • T to C, chromosome 8 at 117,133,025 bp
  • T to A, chromosome 9 at 95,706,933 bp
  • A to G, chromosome 9 at 107,791,090 bp
  • A to T, chromosome 9 at 108,480,610 bp
  • A to G, chromosome 9 at 110,561,243 bp
  • A to G, chromosome 10 at 60,317,128 bp
  • C to T, chromosome 10 at 80,549,569 bp
  • A to T, chromosome 10 at 80,796,472 bp
  • C to A, chromosome 10 at 89,592,864 bp
  • T to A, chromosome 10 at 93,211,958 bp
  • T to C, chromosome 10 at 106,776,410 bp
  • A to G, chromosome 10 at 116,839,326 bp
  • T to A, chromosome 11 at 23,284,634 bp
  • C to T, chromosome 11 at 100,095,767 bp
  • T to A, chromosome 12 at 117,880,025 bp
  • T to C, chromosome 12 at 117,966,639 bp
  • G to A, chromosome 13 at 30,541,107 bp
  • G to A, chromosome 14 at 27,087,994 bp
  • A to G, chromosome 14 at 30,491,016 bp
  • A to G, chromosome 14 at 33,612,311 bp
  • A to G, chromosome 14 at 70,193,656 bp
  • A to G, chromosome 14 at 118,960,820 bp
  • T to C, chromosome 15 at 97,790,573 bp
  • A to G, chromosome 15 at 99,037,876 bp
  • T to A, chromosome 17 at 25,476,511 bp
  • A to G, chromosome 17 at 30,673,835 bp
  • A to T, chromosome 18 at 38,198,657 bp
  • C to T, chromosome 18 at 71,374,551 bp
  • C to T, chromosome 19 at 3,903,591 bp
  • T to C, chromosome 19 at 8,071,208 bp
  • T to C, chromosome 19 at 30,578,745 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2262 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040262-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.