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Strain Name:
C57BL/6J-MtgxR2271Btlr/Mmmh
Stock Number:
040271-MU
Citation ID:
RRID:MMRRC_040271-MU
Other Names:
R2271 (G1), C57BL/6J-MtgxR2271Btlr
Major Collection:

Strain Information

Ap4e1
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108011
HGNC: HGNC:573
Homologene: 22397
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Ntrk3
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18213
HGNC: HGNC:8033
Homologene: 49183
Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Shc1
Name: src homology 2 domain-containing transforming protein C1
Synonyms: p66, ShcA, p66shc
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20416
Homologene: 7934
Usp7
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 252870
Homologene: 2592
Ndufv1
Name: NADH:ubiquinone oxidoreductase core subunit V1
Synonyms: 24 kDa (FP)
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 17995
HGNC: HGNC:7716
Homologene: 5151
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 68,198,888 bp
  • A to G, chromosome 1 at 74,736,687 bp
  • T to G, chromosome 1 at 75,363,493 bp
  • T to C, chromosome 1 at 82,288,459 bp
  • T to A, chromosome 1 at 83,257,221 bp
  • A to T, chromosome 1 at 106,999,026 bp
  • G to A, chromosome 1 at 130,846,662 bp
  • T to C, chromosome 1 at 133,103,428 bp
  • G to A, chromosome 2 at 36,859,625 bp
  • A to G, chromosome 2 at 86,197,817 bp
  • T to C, chromosome 2 at 127,047,163 bp
  • T to A, chromosome 2 at 139,757,373 bp
  • A to T, chromosome 2 at 165,143,847 bp
  • T to A, chromosome 3 at 63,744,535 bp
  • T to A, chromosome 3 at 87,993,094 bp
  • G to A, chromosome 3 at 89,423,542 bp
  • C to T, chromosome 3 at 100,106,797 bp
  • G to A, chromosome 3 at 137,885,380 bp
  • A to G, chromosome 4 at 8,785,532 bp
  • T to C, chromosome 4 at 24,535,936 bp
  • T to A, chromosome 4 at 33,500,302 bp
  • T to A, chromosome 4 at 41,724,744 bp
  • A to C, chromosome 4 at 70,266,678 bp
  • C to T, chromosome 4 at 148,271,602 bp
  • T to C, chromosome 5 at 8,121,108 bp
  • C to A, chromosome 5 at 21,399,721 bp
  • T to G, chromosome 5 at 30,116,974 bp
  • G to T, chromosome 5 at 32,293,242 bp
  • T to A, chromosome 5 at 43,979,330 bp
  • A to T, chromosome 5 at 138,161,141 bp
  • A to G, chromosome 6 at 57,114,103 bp
  • C to A, chromosome 7 at 4,603,133 bp
  • T to A, chromosome 7 at 4,758,187 bp
  • T to C, chromosome 7 at 6,189,555 bp
  • T to A, chromosome 7 at 29,989,122 bp
  • A to T, chromosome 7 at 43,792,172 bp
  • G to T, chromosome 7 at 55,899,957 bp
  • A to C, chromosome 7 at 78,516,723 bp
  • C to T, chromosome 7 at 90,224,077 bp
  • T to G, chromosome 7 at 98,626,623 bp
  • G to C, chromosome 7 at 99,813,443 bp
  • T to A, chromosome 7 at 119,975,129 bp
  • T to A, chromosome 7 at 141,637,510 bp
  • T to C, chromosome 8 at 36,231,198 bp
  • T to A, chromosome 8 at 72,733,419 bp
  • T to A, chromosome 9 at 20,171,409 bp
  • T to A, chromosome 9 at 64,330,883 bp
  • T to C, chromosome 9 at 79,819,930 bp
  • T to G, chromosome 9 at 86,494,418 bp
  • T to C, chromosome 9 at 95,491,420 bp
  • T to C, chromosome 9 at 109,102,708 bp
  • T to A, chromosome 10 at 28,981,579 bp
  • T to C, chromosome 10 at 62,417,898 bp
  • C to T, chromosome 10 at 75,245,604 bp
  • C to T, chromosome 10 at 82,225,547 bp
  • T to C, chromosome 10 at 83,191,170 bp
  • A to G, chromosome 10 at 85,313,731 bp
  • G to A, chromosome 10 at 128,200,158 bp
  • A to G, chromosome 11 at 51,716,450 bp
  • T to C, chromosome 11 at 52,032,495 bp
  • A to T, chromosome 11 at 61,328,526 bp
  • T to C, chromosome 11 at 66,112,362 bp
  • T to A, chromosome 11 at 75,495,363 bp
  • T to A, chromosome 11 at 94,558,873 bp
  • C to G, chromosome 11 at 98,701,427 bp
  • C to T, chromosome 11 at 103,387,122 bp
  • T to C, chromosome 11 at 103,614,207 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • T to A, chromosome 12 at 3,977,116 bp
  • A to T, chromosome 12 at 84,009,981 bp
  • TCATTGTCC to TCATTGTCCATTGTCC, chromosome 13 at 47,064,088 bp
  • A to T, chromosome 13 at 67,592,233 bp
  • A to G, chromosome 14 at 61,204,660 bp
  • T to A, chromosome 15 at 83,946,999 bp
  • T to C, chromosome 16 at 8,698,469 bp
  • A to G, chromosome 16 at 15,654,817 bp
  • T to C, chromosome 16 at 31,956,951 bp
  • G to A, chromosome 16 at 36,031,221 bp
  • T to A, chromosome 16 at 77,076,429 bp
  • A to T, chromosome 17 at 12,586,805 bp
  • A to G, chromosome 17 at 32,821,803 bp
  • A to G, chromosome 17 at 35,044,390 bp
  • A to T, chromosome 17 at 49,449,109 bp
  • C to T, chromosome 17 at 66,555,704 bp
  • T to C, chromosome 17 at 74,602,971 bp
  • A to G, chromosome 17 at 80,217,246 bp
  • G to A, chromosome 17 at 85,063,792 bp
  • C to A, chromosome 19 at 4,008,347 bp
  • T to C, chromosome 19 at 18,703,370 bp
  • T to A, chromosome 19 at 34,510,890 bp
  • C to T, chromosome 19 at 46,005,682 bp
  • C to T, chromosome X at 36,198,465 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2271 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040271-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.