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Strain Name:
C57BL/6J-MtgxR2272Btlr/Mmmh
Stock Number:
040272-MU
Citation ID:
RRID:MMRRC_040272-MU
Other Names:
R2272 (G1), C57BL/6J-MtgxR2272Btlr
Major Collection:

Strain Information

Matr3
Name: matrin 3
Synonyms: D030046F20Rik, 2810017I02Rik, 1110061A14Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17184
HGNC: HGNC:6912
Homologene: 7830
Spns1
Name: SPNS lysolipid transporter 1, lysophospholipid
Synonyms: 2210013K02Rik, spinster homolog
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73658
Homologene: 41530
Ddc
Name: dopa decarboxylase
Synonyms: aromatic L-amino acid decarboxylase, Aadc
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13195
HGNC: HGNC:2719
Homologene: 618
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Prrc1
Name: proline-rich coiled-coil 1
Synonyms: 2310058D16Rik, 9430085A19Rik, 3110038B19Rik, 1190002C06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 73137
VEGA: 18
Homologene: 12491
Usp7
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 252870
Homologene: 2592
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 66,472,140 bp
  • A to G, chromosome 1 at 146,901,404 bp
  • A to T, chromosome 1 at 162,799,047 bp
  • A to T, chromosome 1 at 162,833,855 bp
  • A to T, chromosome 1 at 165,510,297 bp
  • T to C, chromosome 1 at 171,418,060 bp
  • T to A, chromosome 1 at 185,283,542 bp
  • T to A, chromosome 2 at 76,764,520 bp
  • A to T, chromosome 2 at 164,397,242 bp
  • A to T, chromosome 2 at 165,143,847 bp
  • T to C, chromosome 2 at 180,178,603 bp
  • A to G, chromosome 3 at 89,216,671 bp
  • A to G, chromosome 3 at 154,316,762 bp
  • T to A, chromosome 4 at 41,724,744 bp
  • A to C, chromosome 4 at 70,266,678 bp
  • A to G, chromosome 4 at 112,119,868 bp
  • A to G, chromosome 4 at 116,048,668 bp
  • C to A, chromosome 4 at 126,453,650 bp
  • G to A, chromosome 4 at 143,654,142 bp
  • T to A, chromosome 4 at 151,018,885 bp
  • T to C, chromosome 5 at 38,230,550 bp
  • A to G, chromosome 5 at 87,066,255 bp
  • G to A, chromosome 5 at 112,941,622 bp
  • A to G, chromosome 5 at 124,731,466 bp
  • C to T, chromosome 6 at 8,226,865 bp
  • T to G, chromosome 6 at 47,962,521 bp
  • T to C, chromosome 6 at 95,589,094 bp
  • T to A, chromosome 6 at 108,493,755 bp
  • T to A, chromosome 6 at 127,607,298 bp
  • A to G, chromosome 6 at 131,368,572 bp
  • T to C, chromosome 6 at 146,822,114 bp
  • T to A, chromosome 7 at 4,758,187 bp
  • A to T, chromosome 7 at 17,210,696 bp
  • A to C, chromosome 7 at 23,660,191 bp
  • T to A, chromosome 7 at 29,989,122 bp
  • C to A, chromosome 7 at 30,715,500 bp
  • G to T, chromosome 7 at 55,899,957 bp
  • G to A, chromosome 7 at 126,371,165 bp
  • T to A, chromosome 7 at 141,637,510 bp
  • A to T, chromosome 8 at 28,694,756 bp
  • T to C, chromosome 8 at 69,797,164 bp
  • A to G, chromosome 8 at 84,727,175 bp
  • C to T, chromosome 8 at 95,571,107 bp
  • A to T, chromosome 8 at 110,309,132 bp
  • T to A, chromosome 9 at 20,171,409 bp
  • A to G, chromosome 9 at 57,146,785 bp
  • T to A, chromosome 9 at 59,815,301 bp
  • T to C, chromosome 10 at 60,783,297 bp
  • A to G, chromosome 10 at 130,389,114 bp
  • G to A, chromosome 11 at 3,355,807 bp
  • C to A, chromosome 11 at 3,969,524 bp
  • A to T, chromosome 11 at 8,836,197 bp
  • T to C, chromosome 11 at 11,835,764 bp
  • T to C, chromosome 11 at 52,032,495 bp
  • A to T, chromosome 11 at 61,328,526 bp
  • T to C, chromosome 11 at 66,112,362 bp
  • T to A, chromosome 11 at 75,495,363 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • T to A, chromosome 11 at 109,953,638 bp
  • T to C, chromosome 12 at 81,995,314 bp
  • C to T, chromosome 12 at 105,638,008 bp
  • C to T, chromosome 13 at 33,154,541 bp
  • A to T, chromosome 13 at 65,194,408 bp
  • T to C, chromosome 14 at 96,517,908 bp
  • T to C, chromosome 14 at 103,144,338 bp
  • T to A, chromosome 15 at 66,028,680 bp
  • G to A, chromosome 15 at 77,423,710 bp
  • A to G, chromosome 15 at 83,892,962 bp
  • T to A, chromosome 15 at 98,774,347 bp
  • T to C, chromosome 16 at 8,698,469 bp
  • A to G, chromosome 16 at 15,654,817 bp
  • T to C, chromosome 16 at 31,956,951 bp
  • T to C, chromosome 16 at 55,816,941 bp
  • T to G, chromosome 16 at 59,303,444 bp
  • T to C, chromosome 17 at 74,602,971 bp
  • A to G, chromosome 17 at 80,217,246 bp
  • C to T, chromosome 18 at 31,977,043 bp
  • T to A, chromosome 18 at 35,588,325 bp
  • G to T, chromosome 18 at 57,381,646 bp
  • A to G, chromosome 18 at 61,198,578 bp
  • G to T, chromosome 18 at 74,733,925 bp
  • T to C, chromosome 19 at 18,703,370 bp
  • T to A, chromosome 19 at 34,510,890 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2272 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040272-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.