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Strain Name:
C57BL/6J-MtgxR2281Btlr/Mmmh
Stock Number:
040280-MU
Citation ID:
RRID:MMRRC_040280-MU
Other Names:
R2281 (G1), C57BL/6J-MtgxR2281Btlr
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Acadm
Name: acyl-Coenzyme A dehydrogenase, medium chain
Synonyms: MCAD
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11364
HGNC: HGNC:89
Homologene: 3
Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Ece1
Name: endothelin converting enzyme 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230857
HGNC: HGNC:3146
Homologene: 1068
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Smyd1
Name: SET and MYND domain containing 1
Synonyms: Bop, 4632404M21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12180
Homologene: 7645
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 8,595,402 bp
  • T to A, chromosome 1 at 44,056,460 bp
  • A to T, chromosome 1 at 72,192,064 bp
  • A to G, chromosome 1 at 164,195,720 bp
  • A to T, chromosome 1 at 171,256,229 bp
  • A to G, chromosome 1 at 171,389,328 bp
  • T to A, chromosome 2 at 35,283,918 bp
  • T to A, chromosome 2 at 57,112,199 bp
  • G to A, chromosome 2 at 88,987,470 bp
  • T to A, chromosome 2 at 90,471,341 bp
  • T to C, chromosome 2 at 91,679,425 bp
  • T to A, chromosome 2 at 110,682,759 bp
  • A to G, chromosome 2 at 119,903,723 bp
  • G to A, chromosome 3 at 67,399,751 bp
  • A to G, chromosome 3 at 86,776,103 bp
  • C to G, chromosome 3 at 96,559,447 bp
  • T to C, chromosome 3 at 135,601,521 bp
  • A to G, chromosome 3 at 146,646,424 bp
  • A to G, chromosome 3 at 153,933,043 bp
  • G to T, chromosome 4 at 58,082,677 bp
  • A to G, chromosome 4 at 130,166,993 bp
  • C to A, chromosome 4 at 131,808,499 bp
  • C to T, chromosome 4 at 137,946,362 bp
  • G to C, chromosome 4 at 138,395,098 bp
  • A to G, chromosome 4 at 148,489,555 bp
  • A to G, chromosome 4 at 149,344,572 bp
  • T to C, chromosome 4 at 152,135,545 bp
  • A to G, chromosome 4 at 152,557,043 bp
  • A to T, chromosome 4 at 154,984,309 bp
  • A to G, chromosome 5 at 14,540,332 bp
  • A to G, chromosome 5 at 21,831,510 bp
  • C to T, chromosome 5 at 43,737,481 bp
  • C to T, chromosome 5 at 50,001,880 bp
  • T to C, chromosome 5 at 73,041,364 bp
  • T to C, chromosome 5 at 75,637,283 bp
  • T to C, chromosome 5 at 77,059,367 bp
  • T to C, chromosome 5 at 138,298,284 bp
  • G to A, chromosome 6 at 29,438,666 bp
  • T to C, chromosome 6 at 48,458,096 bp
  • C to T, chromosome 6 at 49,417,250 bp
  • A to T, chromosome 6 at 61,570,815 bp
  • T to C, chromosome 6 at 71,238,676 bp
  • A to T, chromosome 6 at 119,963,640 bp
  • C to T, chromosome 7 at 30,958,345 bp
  • G to A, chromosome 7 at 31,053,878 bp
  • T to A, chromosome 7 at 92,438,041 bp
  • G to A, chromosome 7 at 92,625,773 bp
  • A to G, chromosome 7 at 140,861,938 bp
  • T to A, chromosome 8 at 34,115,223 bp
  • C to A, chromosome 8 at 76,909,907 bp
  • A to G, chromosome 8 at 104,125,733 bp
  • T to C, chromosome 8 at 105,387,266 bp
  • T to C, chromosome 8 at 111,918,931 bp
  • T to C, chromosome 8 at 124,948,414 bp
  • C to T, chromosome 9 at 22,076,540 bp
  • T to C, chromosome 10 at 80,713,998 bp
  • T to A, chromosome 10 at 100,603,338 bp
  • T to G, chromosome 10 at 127,048,294 bp
  • A to T, chromosome 11 at 9,328,136 bp
  • T to C, chromosome 11 at 41,976,636 bp
  • A to C, chromosome 11 at 49,293,632 bp
  • T to C, chromosome 11 at 59,549,136 bp
  • A to T, chromosome 11 at 76,237,809 bp
  • T to C, chromosome 11 at 76,797,801 bp
  • A to G, chromosome 11 at 100,698,154 bp
  • G to T, chromosome 11 at 115,802,989 bp
  • A to G, chromosome 12 at 8,978,628 bp
  • A to G, chromosome 13 at 23,738,924 bp
  • G to T, chromosome 14 at 44,989,650 bp
  • C to A, chromosome 14 at 54,577,188 bp
  • G to T, chromosome 14 at 55,569,371 bp
  • T to A, chromosome 14 at 55,971,528 bp
  • T to A, chromosome 14 at 79,064,996 bp
  • A to T, chromosome 15 at 100,353,360 bp
  • T to C, chromosome 16 at 11,158,594 bp
  • C to T, chromosome 16 at 22,961,309 bp
  • T to A, chromosome 17 at 24,376,726 bp
  • A to G, chromosome 18 at 34,808,419 bp
  • T to C, chromosome 18 at 61,262,434 bp
  • T to C, chromosome 18 at 65,624,712 bp
  • T to C, chromosome 19 at 8,909,468 bp
  • T to A, chromosome 19 at 10,551,873 bp
  • A to G, chromosome 19 at 20,620,091 bp
  • T to A, chromosome 19 at 24,378,947 bp
  • G to A, chromosome 19 at 42,283,567 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2281 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040280-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.