Beutler Mutagenetix

Strain Name:
C57BL/6J-MtgxR2286Btlr/Mmmh
Stock Number:
040285-MU
Citation ID:
RRID:MMRRC_040285-MU
Other Names:
R2286 (G1), C57BL/6J-MtgxR2286Btlr
Major Collection:

Gene Information

Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 72895
Homologene: 12485
Vps26c
Name: VPS26 endosomal protein sorting factor C
Synonyms: Down syndrome critical region gene 3, Dcra, Dscr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 13185
HGNC: HGNC:3044
Homologene: 4415
Psmg1
Name: proteasome (prosome, macropain) assembly chaperone 1
Synonyms: Dscr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 56088
HGNC: HGNC:3043
Homologene: 2759
Cpsf7
Name: cleavage and polyadenylation specific factor 7
Synonyms: C330017N18Rik, 5730453I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 269061
VEGA: 19
Homologene: 11731
Dtd1
Name: D-tyrosyl-tRNA deacylase 1
Synonyms: 0610006H08Rik, Hars2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 66044
Homologene: 5518
Med13
Name: mediator complex subunit 13
Synonyms: Trap240, 1110067M05Rik, Thrap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 327987
Homologene: 21067
Kremen1
Name: kringle containing transmembrane protein 1
Synonyms: Krm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 84035
Homologene: 12935
Luc7l
Name: Luc7-like
Synonyms: 1810045C04Rik, 2410018D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 66978
HGNC: HGNC:6723
Homologene: 100558
Smarcc2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
Synonyms: 5930405J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 68094
VEGA: 10
Homologene: 2312
Slc39a5
Name: solute carrier family 39 (metal ion transporter), member 5
Synonyms: 1810013D05Rik, 2010205A06Rik, Zip5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 72002
VEGA: 10
Homologene: 57083
Myo6
Name: myosin VI
Synonyms: rsv, Tlc, Myo6rsv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17920
HGNC: HGNC:7605
Homologene: 56417
Tnn
Name: tenascin N
Synonyms: Tnw, tenascin-W
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 329278
Homologene: 18634
Acss3
Name: acyl-CoA synthetase short-chain family member 3
Synonyms: LOC380660, 8430416H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 380660
Homologene: 11587
Naip6
Name: NLR family, apoptosis inhibitory protein 6
Synonyms: Birc1f, Naip-rs4, Naip-rs4A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 17952
HGNC: HGNC:7634
Homologene: 113589
Alox5ap
Name: arachidonate 5-lipoxygenase activating protein
Synonyms: Flap, arachidonate 5 lipoxygenase activating protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11690
HGNC: HGNC:436
Homologene: 1231
Olfr1281
Name: olfactory receptor 1281
Synonyms: MOR248-14P, MOR248-18, GA_x6K02T2Q125-72379864-72380781
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 257979
HGNC: null
Homologene: 73992
Unc13a
Name: unc-13 homolog A
Synonyms: Munc13-1, 2410078G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 382018
Homologene: 11279
Rsad2
Name: radical S-adenosyl methionine domain containing 2
Synonyms: viperin, cig5, 2510004L01Rik, Vig1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 58185
Homologene: 10969
Tdrd1
Name: tudor domain containing 1
Synonyms: MTR-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 83561
Homologene: 12850
Cdr2l
Name: cerebellar degeneration-related protein 2-like
Synonyms: D030068L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237988
Homologene: 35390
Adam22
Name: a disintegrin and metallopeptidase domain 22
Synonyms: 2900022I03Rik, MDC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11496
HGNC: HGNC:201
Homologene: 37898
Slc28a1
Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 1
Synonyms: Cnt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 434203
Homologene: 37902
Vmn2r120
Name: vomeronasal 2, receptor 120
Synonyms: EG224916
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224916
HGNC: null
Homologene: null
Ap2s1
Name: adaptor-related protein complex 2, sigma 1 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 232910
HGNC: HGNC:565
Homologene: 3001
Sgip1
Name: SH3-domain GRB2-like (endophilin) interacting protein 1
Synonyms: 3110007P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 73094
Homologene: 13001
Eci1
Name: enoyl-Coenzyme A delta isomerase 1
Synonyms: eci, Dci
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 13177
VEGA: 17
HGNC: HGNC:2703
Homologene: 37554
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 160,110,509 bp
  • A to G, chromosome 2 at 111,328,907 bp
  • T to C, chromosome 2 at 144,635,866 bp
  • G to T, chromosome 4 at 102,867,647 bp
  • C to T, chromosome 5 at 8,145,616 bp
  • G to A, chromosome 5 at 149,285,430 bp
  • T to G, chromosome 6 at 113,119,610 bp
  • T to C, chromosome 7 at 16,748,976 bp
  • T to C, chromosome 7 at 81,126,148 bp
  • T to C, chromosome 8 at 71,630,559 bp
  • T to C, chromosome 9 at 80,266,212 bp
  • T to C, chromosome 10 at 106,937,332 bp
  • A to G, chromosome 10 at 128,396,060 bp
  • G to A, chromosome 10 at 128,463,743 bp
  • CGGG to CGGGGGG, chromosome 11 at 5,201,791 bp
  • A to C, chromosome 11 at 86,319,689 bp
  • GAA to GA, chromosome 11 at 115,392,800 bp
  • T to A, chromosome 12 at 26,450,676 bp
  • C to T, chromosome 13 at 100,300,600 bp
  • C to T, chromosome 16 at 94,512,253 bp
  • G to T, chromosome 16 at 95,982,092 bp
  • A to G, chromosome 17 at 24,433,229 bp
  • A to G, chromosome 17 at 26,280,046 bp
  • A to G, chromosome 17 at 57,508,958 bp
  • T to C, chromosome 19 at 10,535,296 bp
  • A to G, chromosome 19 at 56,839,119 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2286 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040285-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.