Strain Name:
C57BL/6J-MtgxR2287Btlr/Mmmh
Stock Number:
040286-MU
Citation ID:
RRID:MMRRC_040286-MU
Other Names:
R2287 (G1), C57BL/6J-MtgxR2287Btlr
Major Collection:

Strain Information

Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74996
Homologene: 9929
Nsf
Name: N-ethylmaleimide sensitive fusion protein
Synonyms: SKD2, N-ethylmaleimide sensitive factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18195
HGNC: HGNC:8016
Homologene: 4502
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209018
Homologene: 44592
Dnajc10
Name: DnaJ heat shock protein family (Hsp40) member C10
Synonyms: JPDI, D2Ertd706e, 1200006L06Rik, ERdj5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66861
Homologene: 10358
Gsdma3
Name: gasdermin A3
Synonyms: Rco2, Bsk, Fgn, Dfl, Rim3, Gsdm1l, Gsdm3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 450219
Itsn2
Name: intersectin 2
Synonyms: Sh3p18, Ese2, Eh domain, SH3 domain regulator of endocytosis 2, Sh3d1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20403
VEGA: 12
HGNC: HGNC:6184
Homologene: 22627
Plxdc2
Name: plexin domain containing 2
Synonyms: 1200007L24Rik, Tem7r, 5430431D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67448
Homologene: 41666
Pcolce2
Name: procollagen C-endopeptidase enhancer 2
Synonyms: Pcpe2, 2400001O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76477
HGNC: HGNC:8739
Homologene: 8357
Clec4f
Name: C-type lectin domain family 4, member f
Synonyms: kupffer cell receptor, D18063, Clecsf13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 51811
Homologene: 9630
Pcnx4
Name: pecanex homolog 4
Synonyms: 1810048J11Rik, Pcnxl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 67708
VEGA: 12
Homologene: 23366
Nlrp14
Name: NLR family, pyrin domain containing 14
Synonyms: 4921520L01Rik, Nalp14, GC-LRR, Nalp-iota
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76858
Homologene: 18531
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: oxygen-regulated protein 1, Orp1, mG145, Dcdc3, Rp1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19888
Homologene: 4564
Dis3l
Name: DIS3 like exosome 3'-5' exoribonuclease
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 213550
Homologene: 15797
Top2b
Name: topoisomerase (DNA) II beta
Synonyms: Top-2, D230016L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21974
Homologene: 134711
Speg
Name: SPEG complex locus
Synonyms: BPEG, SPEGbeta, SPEGalpha, D1Bwg1450e, SPEG, Apeg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11790
Homologene: 55619
Naalad2
Name: N-acetylated alpha-linked acidic dipeptidase 2
Synonyms: NAALADASE2, NAADALASE2, D9Ertd285e, GCPIII, GCP3, Folh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72560
Homologene: 3294
Gtf2h4
Name: general transcription factor II H, polypeptide 4
Synonyms: p52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14885
HGNC: HGNC:4658
Homologene: 7090
Trim34a
Name: tripartite motif-containing 34A
Synonyms: Trim34-1, Trim34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 94094
Homologene: 128238
Ndufa8
Name: NADH:ubiquinone oxidoreductase subunit A8
Synonyms: 0610033L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68375
HGNC: HGNC:7692
Homologene: 40932
Ehf
Name: ets homologous factor
Synonyms: 9030625L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13661
HGNC: HGNC:3246
Homologene: 7301
Kcnd3
Name: potassium voltage-gated channel, Shal-related family, member 3
Synonyms: potassium channel Kv4.3M, potassium channel Kv4.3L, Kv4.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56543
HGNC: HGNC:6239
Homologene: 21036
Skint4
Name: selection and upkeep of intraepithelial T cells 4
Synonyms: 9530098N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320640
Edem2
Name: ER degradation enhancer, mannosidase alpha-like 2
Synonyms: 9530090G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 108687
Homologene: 10075
Trpv6
Name: transient receptor potential cation channel, subfamily V, member 6
Synonyms: CaT1, Cac, CAT, Ecac2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 64177
Homologene: 56812
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 4,345,959 bp
  • A to G, chromosome 1 at 75,430,465 bp
  • A to G, chromosome 2 at 16,512,190 bp
  • C to T, chromosome 2 at 36,036,542 bp
  • T to G, chromosome 2 at 80,331,418 bp
  • T to C, chromosome 2 at 103,267,124 bp
  • T to C, chromosome 2 at 155,713,359 bp
  • C to T, chromosome 3 at 105,658,766 bp
  • A to G, chromosome 4 at 112,118,205 bp
  • T to G, chromosome 6 at 41,626,111 bp
  • CTT to CT, chromosome 6 at 83,653,265 bp
  • T to A, chromosome 7 at 104,261,055 bp
  • A to T, chromosome 7 at 107,182,662 bp
  • T to C, chromosome 7 at 112,056,626 bp
  • A to T, chromosome 9 at 18,335,021 bp
  • G to T, chromosome 9 at 18,335,022 bp
  • T to A, chromosome 9 at 64,307,497 bp
  • G to A, chromosome 9 at 95,678,405 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • A to G, chromosome 11 at 98,638,004 bp
  • C to T, chromosome 11 at 103,930,752 bp
  • T to C, chromosome 12 at 4,708,105 bp
  • T to C, chromosome 12 at 72,575,398 bp
  • T to G, chromosome 14 at 16,409,189 bp
  • T to C, chromosome 16 at 21,568,413 bp
  • C to A, chromosome 17 at 35,671,225 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2287 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040286-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.