Strain Name:
C57BL/6J-MtgxR2297Btlr/Mmmh
Stock Number:
040296-MU
Citation ID:
RRID:MMRRC_040296-MU
Other Names:
R2297 (G1), C57BL/6J-MtgxR2297Btlr
Major Collection:

Gene Information

Grm1
Name: glutamate receptor, metabotropic 1
Synonyms: mGluR1, Grm1, Gprc1a, nmf373, rcw, 4930455H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14816
HGNC: HGNC:4593
Homologene: 649
Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228852
Homologene: 9194
Aco2
Name: aconitase 2, mitochondrial
Synonyms: Aco3, Aco-2, D10Wsu183e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11429
HGNC: HGNC:118
Homologene: 856
Cop1
Name: COP1, E3 ubiquitin ligase
Synonyms: Cop1, Rfwd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26374
Homologene: 115565
Dhx9
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 9
Synonyms: leukophysin, nuclear DNA helicase II, RNA helicase, Ddx9, NDH II, NDHII, RHA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13211
HGNC: HGNC:2750
Homologene: 1039
Ddx42
Name: DEAD box helicase 42
Synonyms: B430002H05Rik, 1810047H21Rik, SF3b125, DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72047
Homologene: 49137
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Ncapd3
Name: non-SMC condensin II complex, subunit D3
Synonyms: 4632407J06Rik, 2810487N22Rik, B130055D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 78658
VEGA: 9
Homologene: 41021
Slc6a11
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 11
Synonyms: GAT4, D930045G19Rik, E130202I16Rik, Gabt4, Gat3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243616
Homologene: 129935
H3f3a
Name: H3.3 histone A
Synonyms: H3.3A, H3-3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 15078
Homologene: 134170
Dtl
Name: denticleless E3 ubiquitin protein ligase
Synonyms: 5730564G15Rik, 2810047L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76843
Homologene: 32313
Dcaf6
Name: DDB1 and CUL4 associated factor 6
Synonyms: PC326, 1200006M05Rik, Iqwd1, NRIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74106
Homologene: 10199
Fem1c
Name: fem 1 homolog c
Synonyms: 3632443A22Rik, 2610312A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240263
Homologene: 10606
Galnt12
Name: polypeptide N-acetylgalactosaminyltransferase 12
Synonyms: A630062B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230145
Homologene: 11637
Stab2
Name: stabilin 2
Synonyms: STAB-2, FEEL-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 192188
Homologene: 23022
Dlgap2
Name: DLG associated protein 2
Synonyms: SAP90/PSD-95-associated protein 2, PSD-95/SAP90-binding protein 2, 6430596N04Rik, DAP2, Sapap2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244310
HGNC: HGNC:2906
Homologene: 3484
Tmem132d
Name: transmembrane protein 132D
Synonyms: C630028F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243274
Homologene: 71684
4921536K21Rik
Name: RIKEN cDNA 4921536K21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67430
Homologene: 12129
Zfp119b
Name: zinc finger protein 119b
Synonyms: BC031441
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240120
Trdn
Name: triadin
Synonyms: 2310045H21Rik, triadin-3, triadin-2, triadin-1, triadin 3, triadin 2, triadin 1, EG432451
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76757
VEGA: 10
Homologene: 38137
Tnfrsf13b
Name: tumor necrosis factor receptor superfamily, member 13b
Synonyms: Taci, 1200009E08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 57916
Homologene: 49320
Cyp2a12
Name: cytochrome P450, family 2, subfamily a, polypeptide 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13085
Homologene: 69128
Pimreg
Name: PICALM interacting mitotic regulator
Synonyms: 2610008F03Rik, CATS, 6720460F02Rik, Fam64a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 109212
Homologene: 10378
Olfr368
Name: olfactory receptor 368
Synonyms: GA_x6K02T2NLDC-34015743-34016726, MOR178-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258371
HGNC: HGNC:8331
Homologene: 71970
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 153,472,572 bp
  • C to T, chromosome 1 at 159,252,554 bp
  • T to C, chromosome 1 at 165,399,862 bp
  • C to T, chromosome 1 at 180,803,138 bp
  • A to G, chromosome 1 at 191,541,095 bp
  • A to G, chromosome 2 at 37,332,143 bp
  • G to A, chromosome 2 at 158,761,366 bp
  • T to A, chromosome 4 at 47,113,834 bp
  • T to C, chromosome 5 at 128,268,544 bp
  • A to G, chromosome 6 at 114,131,425 bp
  • A to G, chromosome 7 at 27,034,632 bp
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp
  • T to C, chromosome 8 at 14,843,665 bp
  • C to T, chromosome 9 at 27,041,501 bp
  • T to G, chromosome 10 at 11,080,414 bp
  • C to A, chromosome 10 at 33,335,012 bp
  • T to A, chromosome 10 at 86,954,474 bp
  • G to A, chromosome 11 at 3,890,127 bp
  • A to G, chromosome 11 at 61,147,445 bp
  • T to C, chromosome 11 at 72,043,080 bp
  • T to G, chromosome 11 at 106,242,939 bp
  • A to T, chromosome 15 at 81,903,908 bp
  • T to A, chromosome 17 at 55,939,355 bp
  • T to C, chromosome 18 at 46,506,161 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2297 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040296-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.