Strain Name:
C57BL/6J-MtgxR2299Btlr/Mmmh
Stock Number:
040298-MU
Citation ID:
RRID:MMRRC_040298-MU
Other Names:
R2299 (G1), C57BL/6J-MtgxR2299Btlr
Major Collection:

Gene Information

Abca12
Name: ATP-binding cassette, sub-family A (ABC1), member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74591
Homologene: 45441
Ptpn23
Name: protein tyrosine phosphatase, non-receptor type 23
Synonyms: PTP-TD14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 104831
Homologene: 135706
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Cyfip2
Name: cytoplasmic FMR1 interacting protein 2
Synonyms: 1500004I01Rik, Pir121, 6430511D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76884
Homologene: 7936
Rnf14
Name: ring finger protein 14
Synonyms: Triad2, 2610005D23Rik, 2310075C09Rik, D18Ertd188e, D7Bwg0165e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 56736
VEGA: 18
Homologene: 129170
H3f3a
Name: H3.3 histone A
Synonyms: H3.3A, H3-3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 15078
Homologene: 134170
C1qtnf6
Name: C1q and tumor necrosis factor related protein 6
Synonyms: 2810036M19Rik, CTRP6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72709
Homologene: 12481
Treml1
Name: triggering receptor expressed on myeloid cells-like 1
Synonyms: TLT-1, 5430401J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 71326
VEGA: 17
Homologene: 52257
Adgrg5
Name: adhesion G protein-coupled receptor G5
Synonyms: LOC382045, PGR27, Gpr114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382045
Homologene: 17828
Limd1
Name: LIM domains containing 1
Synonyms: D9Ertd192e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 29806
VEGA: 9
HGNC: HGNC:6612
Homologene: 8478
Chordc1
Name: cysteine and histidine-rich domain (CHORD)-containing, zinc-binding protein 1
Synonyms: 1110001O09Rik, Chp-1, morgana
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66917
VEGA: 9
Homologene: 8111
Acsl3
Name: acyl-CoA synthetase long-chain family member 3
Synonyms: 2610510B12Rik, C85929, Facl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74205
HGNC: HGNC:3570
Homologene: 3278
Usp33
Name: ubiquitin specific peptidase 33
Synonyms: Vdu1, 9830169D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170822
Homologene: 8996
Stk38
Name: serine/threonine kinase 38
Synonyms: 5830476G13Rik, 9530097A09Rik, Ndr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106504
Homologene: 56033
Ppwd1
Name: peptidylprolyl isomerase domain and WD repeat containing 1
Synonyms: A330090G21Rik, 4632422M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238831
VEGA: 13
Homologene: 9099
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 545260
Homologene: 44428
Bmpr1b
Name: bone morphogenetic protein receptor, type 1B
Synonyms: Alk6, CFK-43a, Acvrlk6, BMPR-IB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12167
HGNC: HGNC:1077
Homologene: 20322
Abcc6
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 6
Synonyms: DCC, Mrp6, Dyscalc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 27421
HGNC: HGNC:57
Homologene: 55559
Kansl1l
Name: KAT8 regulatory NSL complex subunit 1-like
Synonyms: C430010P07Rik, 1110028C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68691
Homologene: 27376
Folr1
Name: folate receptor 1 (adult)
Synonyms: FBP1, folate receptor [a], folate-binding protein 1, Folbp1, Folbp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14275
HGNC: HGNC:3791
Homologene: 7322
Dsg1a
Name: desmoglein 1 alpha
Synonyms: Dsg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13510
HGNC: HGNC:3048
Homologene: 1463
Ankrd42
Name: ankyrin repeat domain 42
Synonyms: 4933417L02Rik, Ikbn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73845
Homologene: 23652
Galnt13
Name: polypeptide N-acetylgalactosaminyltransferase 13
Synonyms: pp-GalNAc-T13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271786
Homologene: 62167
Mmrn1
Name: multimerin 1
Synonyms: 4921530G03Rik, Emilin4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 70945
HGNC: HGNC:7178
Homologene: 49134
Sema5a
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms: M-Sema D, semF, Semaf, 9130201M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20356
VEGA: 15
Homologene: 2949
Slc28a2
Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 2
Synonyms: CNT2, 2010208B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269346
Homologene: 20891
Vcpip1
Name: valosin containing protein (p97)/p47 complex interacting protein 1
Synonyms: 5730421J18Rik, 5730538E15Rik, Vcip135
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70675
Homologene: 11814
5031439G07Rik
Name: RIKEN cDNA 5031439G07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223739
HGNC: HGNC:1314
Homologene: 15140
1700094D03Rik
Name: RIKEN cDNA 1700094D03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 73545
VEGA: 3
Homologene: 78671
Spire1
Name: spire type actin nucleation factor 1
Synonyms: Spir-1, 6030430B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 68166
VEGA: 18
Homologene: 35507
Clec2j
Name: C-type lectin domain family 2, member J
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 677440
Fbxl7
Name: F-box and leucine-rich repeat protein 7
Synonyms: Fbl6, FBL7, D230018M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 448987
VEGA: 15
Homologene: 69121
Zbtb24
Name: zinc finger and BTB domain containing 24
Synonyms: ZNF450
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 268294
VEGA: 10
Homologene: 8870
Vmn1r174
Name: vomeronasal 1 receptor 174
Synonyms: V1rd22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404291
Homologene: 79577
Olfr583
Name: olfactory receptor 583
Synonyms: GA_x6K02T2PBJ9-5762668-5763618, MOR14-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258752
Homologene: 133721
Prss21
Name: protease, serine 21
Synonyms: testisin, 1700023E12Rik, mT4, TESP5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57256
HGNC: HGNC:9485
Homologene: 137342
Abhd17a
Name: abhydrolase domain containing 17A
Synonyms: 1700013O15Rik, D10Bwg1364e, Fam108a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216169
Homologene: 50272
Gm4559
Name: predicted gene 4559
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043627
VEGA: 7
Rit1
Name: Ras-like without CAAX 1
Synonyms: Rit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19769
Homologene: 56003
Crygs
Name: crystallin, gamma S
Synonyms: Opj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12970
VEGA: 16
HGNC: HGNC:2417
Homologene: 40695
4930415L06Rik
Name: RIKEN cDNA 4930415L06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245511
Homologene: 133109
H2bc9
Name: H2B clustered histone 9
Synonyms: Hist1h2bh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 319182
Homologene: 138447
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 9,745,719 bp
  • T to C, chromosome 1 at 66,773,477 bp
  • C to T, chromosome 1 at 71,258,222 bp
  • T to G, chromosome 1 at 78,699,110 bp
  • T to C, chromosome 1 at 172,121,725 bp
  • C to T, chromosome 1 at 180,803,138 bp
  • A to T, chromosome 2 at 55,060,583 bp
  • C to T, chromosome 2 at 122,441,778 bp
  • T to A, chromosome 3 at 88,726,070 bp
  • A to G, chromosome 3 at 90,064,538 bp
  • C to A, chromosome 3 at 141,845,202 bp
  • T to A, chromosome 3 at 152,374,621 bp
  • A to G, chromosome 6 at 60,976,441 bp
  • T to A, chromosome 6 at 128,655,236 bp
  • A to T, chromosome 7 at 23,754,004 bp
  • G to A, chromosome 7 at 46,005,649 bp
  • A to G, chromosome 7 at 92,590,254 bp
  • A to G, chromosome 7 at 101,863,992 bp
  • T to C, chromosome 7 at 103,051,582 bp
  • A to T, chromosome 7 at 142,273,835 bp
  • A to T, chromosome 8 at 94,938,576 bp
  • T to C, chromosome 9 at 18,302,108 bp
  • A to G, chromosome 9 at 110,392,513 bp
  • A to T, chromosome 9 at 123,516,877 bp
  • G to A, chromosome 10 at 41,464,581 bp
  • G to T, chromosome 10 at 80,584,129 bp
  • T to A, chromosome 11 at 46,286,131 bp
  • A to G, chromosome 13 at 23,543,184 bp
  • T to C, chromosome 13 at 104,220,063 bp
  • C to T, chromosome 15 at 26,895,001 bp
  • T to A, chromosome 15 at 32,562,776 bp
  • T to A, chromosome 15 at 78,525,342 bp
  • A to C, chromosome 15 at 84,953,285 bp
  • C to T, chromosome 16 at 22,805,551 bp
  • A to G, chromosome 17 at 23,869,589 bp
  • G to A, chromosome 17 at 28,975,946 bp
  • A to G, chromosome 17 at 48,365,604 bp
  • A to T, chromosome 18 at 20,340,150 bp
  • C to T, chromosome 18 at 38,308,085 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • A to C, chromosome 18 at 67,530,423 bp
  • A to G, chromosome X at 89,932,399 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2299 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040298-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.