Strain Name:
C57BL/6J-MtgxR2300Btlr/Mmmh
Stock Number:
040299-MU
Citation ID:
RRID:MMRRC_040299-MU
Other Names:
R2300 (G1), C57BL/6J-MtgxR2300Btlr
Major Collection:

Gene Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 47, Todo, Obt, Mt, Lda, A730019I05Rik, Whi, Flo, Gena 52, Edy, GENA 60, Cyn, Dz, WBE1, Cycn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320790
Homologene: 19067
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, A930013E17Rik, Zap3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56531
Homologene: 87707
Tesk2
Name: testis-specific kinase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230661
Homologene: 5188
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: PD-Ialpha, ATX, Pdnp2, Autotaxin, Npps2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Kif11
Name: kinesin family member 11
Synonyms: Eg5, Kifl1, Knsl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 16551
VEGA: 19
HGNC: HGNC:6388
Homologene: 3322
Arid2
Name: AT rich interactive domain 2 (ARID, RFX-like)
Synonyms: zipzap/p200, 4432409D24Rik, 1700124K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77044
Homologene: 14601
Stag1
Name: stromal antigen 1
Synonyms: Scc3, SA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20842
Homologene: 21191
Xdh
Name: xanthine dehydrogenase
Synonyms: XO, Xox1, xanthine oxidase, Xox-1, Xor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22436
VEGA: 17
Homologene: 324
Adgrl1
Name: adhesion G protein-coupled receptor L1
Synonyms: lectomedin-2, 2900070I05Rik, Lphn1, Lec2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330814
Homologene: 8951
H3f3a
Name: H3.3 histone A
Synonyms: H3-3a, H3.3A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 15078
Homologene: 134170
Clca4b
Name: chloride channel accessory 4B
Synonyms: AI747448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99709
HGNC: HGNC:2018
Homologene: 40808
Nop16
Name: NOP16 nucleolar protein
Synonyms: D13Wsu177e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 28126
Homologene: 16887
Ostf1
Name: osteoclast stimulating factor 1
Synonyms: Sh3d3, SH3P2, C78236
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20409
HGNC: HGNC:8510
Homologene: 8227
Cntrl
Name: centriolin
Synonyms: 6720467O09Rik, Ma2a8, Cep110, IB3/5, b2b1468Clo, Cep1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26920
HGNC: HGNC:1858
Homologene: 38260
Nolc1
Name: nucleolar and coiled-body phosphoprotein 1
Synonyms: 3230402K17Rik, NOPP140, P130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 70769
VEGA: 19
Jag1
Name: jagged 1
Synonyms: ABE2, Gsfabe2, Htu, Headturner, Ozz, Serrate-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16449
HGNC: HGNC:6188
Homologene: 180
Ttn
Name: titin
Synonyms: 2310057K23Rik, connectin, D830007G01Rik, D330041I19Rik, L56, shru, 1100001C23Rik, 2310036G12Rik, mdm, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: A2mr, CD91, b2b1554Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Lama4
Name: laminin, alpha 4
Synonyms: laminin [a]4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16775
HGNC: HGNC:6484
Homologene: 37604
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 545260
Homologene: 44428
Aldh6a1
Name: aldehyde dehydrogenase family 6, subfamily A1
Synonyms: Mmsdh, 1110038I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104776
VEGA: 12
HGNC: HGNC:7179
Homologene: 4082
St18
Name: suppression of tumorigenicity 18
Synonyms: Myt3, Nzf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240690
Homologene: 8792
Cd53
Name: CD53 antigen
Synonyms: Ox-44, Tspan25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12508
HGNC: HGNC:1686
Homologene: 20152
Olfr992
Name: olfactory receptor 992
Synonyms: MOR203-1, GA_x6K02T2Q125-46877170-46876241
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258865
Homologene: 121517
Gabrr1
Name: gamma-aminobutyric acid (GABA) C receptor, subunit rho 1
Synonyms: GABA-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14408
HGNC: HGNC:4090
Homologene: 20470
Tsen54
Name: tRNA splicing endonuclease subunit 54
Synonyms: 0610034P02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76265
Homologene: 35476
Olfr134
Name: olfactory receptor 134
Synonyms: MOR256-5, GA_x6K02T2PSCP-2623613-2624551
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258829
Homologene: 110603
Slc28a2
Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 2
Synonyms: CNT2, 2010208B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269346
Homologene: 20891
Mrc2
Name: mannose receptor, C type 2
Synonyms: uPARAP, novel lectin, Endo180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17534
Homologene: 4408
Arhgap24
Name: Rho GTPase activating protein 24
Synonyms: 0610025G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231532
Homologene: 32754
Tcl1b1
Name: T cell leukemia/lymphoma 1B, 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 27379
VEGA: 12
Homologene: 83274
Crygs
Name: crystallin, gamma S
Synonyms: Opj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12970
VEGA: 16
HGNC: HGNC:2417
Homologene: 40695
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 6,855,402 bp
  • C to T, chromosome 1 at 180,803,138 bp
  • A to G, chromosome 2 at 35,127,513 bp
  • A to G, chromosome 2 at 76,907,448 bp
  • T to C, chromosome 2 at 85,400,132 bp
  • C to T, chromosome 2 at 122,441,778 bp
  • T to C, chromosome 2 at 137,096,315 bp
  • T to C, chromosome 3 at 106,763,256 bp
  • A to T, chromosome 3 at 144,916,671 bp
  • G to A, chromosome 4 at 8,855,241 bp
  • A to G, chromosome 4 at 33,152,449 bp
  • G to A, chromosome 4 at 116,771,856 bp
  • A to G, chromosome 5 at 102,860,425 bp
  • G to T, chromosome 8 at 83,930,117 bp
  • T to C, chromosome 9 at 100,712,500 bp
  • T to A, chromosome 10 at 39,087,320 bp
  • G to T, chromosome 10 at 127,556,915 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • A to T, chromosome 11 at 115,822,078 bp
  • G to T, chromosome 12 at 84,439,529 bp
  • G to A, chromosome 12 at 85,060,319 bp
  • G to A, chromosome 12 at 105,164,524 bp
  • A to G, chromosome 13 at 54,585,866 bp
  • A to G, chromosome 15 at 54,877,936 bp
  • A to T, chromosome 15 at 96,402,006 bp
  • C to T, chromosome 16 at 22,805,551 bp
  • T to A, chromosome 17 at 38,175,550 bp
  • A to G, chromosome 17 at 73,891,265 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • T to C, chromosome 19 at 18,581,280 bp
  • A to G, chromosome 19 at 37,411,539 bp
  • CAG to CAGAAG, chromosome 19 at 46,081,359 bp
  • CAG to CAGTAG, chromosome 19 at 46,081,368 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2300 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040299-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.